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Extracellular-nucleotide metabolisms of oral microorganisms

口腔微生物的细胞外核苷酸代谢

基本信息

批准号：
10671764
负责人：
ABE Kazuhiko
金额：
$ 1.98万
依托单位：
Tohoku Fukushi University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

项目摘要

It has been reported that various bacteria inhabit in an oral cavity and some of these bacteria may cause several diseases in the other area. Especially Streptococcus sanguinis has been detected in the inflammation part of endocarditis and in various abscess regions. In this study we examined whether oral streptococci metabolize extra-cellular nucleotide which is supposed to exist in abscess regions. As a result, it was confirmed that Streptococcus sanguinis, Streptococcus gordonii and Streptococcus salivarius metabolized CMP and produced the ammonia and inorganic phosphate. Therefore, we examined the metabolic difference both these two streptococcal groups in detail. Both bacteria group had significant degree of cytidine deaminase activity in the cell-free extracts. Although S. sanguinis, S. gordonii and S. salivarius contained membrane-bound 5'-nucleotidase activity, but mutans streptococci did not contain the activity. The 5'-nucleotidase activity in the cell-free extract of S. sanguinis fell off significantly by the oxygen flash. Certain kind of active oxygen may be generated and inactivate the 5'-nucleotidase. AMP, GMP, UMP, deoxyAMP, deoxyCMP, deoxyGMP were decomposed by S sanguinis cells and inorganic phosphate was produced. And it was confirmed that S. sanguinis cells generated ammonia from (deoxy-)AMP, (deoxy-)CMP. In this study we found that S. sanguinis, S. gordonii and S. salivarius could metabolize extra-cellular 5'-(deoxy) ribonucleotide. It was considered that these facts are important to understand the ecosystem of the bacteria in various regions of the body as well as the dental plaque.

据报道，口腔内栖息着多种细菌，其中一些细菌可能会引起其他部位的多种疾病。特别是在心内膜炎的炎症部分和各种脓肿区域中检测到了血链球菌。在这项研究中，我们检查了口腔链球菌是否代谢脓肿区域中被认为存在的细胞外核苷酸。结果证实，血链球菌、戈登链球菌和唾液链球菌代谢CMP并产生氨和无机磷酸盐。因此，我们详细检查了这两个链球菌群的代谢差异。两个细菌组的无细胞提取物中都具有显着程度的胞苷脱氨酶活性。尽管血链球菌、戈登链球菌和唾液链球菌含有膜结合5'-核苷酸酶活性，但变形链球菌不含有该活性。血链球菌无细胞提取物中的 5'-核苷酸酶活性因氧气闪蒸而显着下降。可能会产生某种活性氧并使5'-核苷酸酶失活。 AMP、GMP、UMP、deoxyAMP、deoxyCMP、deoxyGMP被血链球菌细胞分解，产生无机磷酸盐。并证实血链球菌细胞从(脱氧-)AMP、(脱氧-)CMP产生氨。在本研究中，我们发现S. sanguinis、S. gordonii 和S. salivarius 可以代谢细胞外5'-(脱氧)核糖核苷酸。人们认为这些事实对于了解身体各个区域的细菌生态系统以及牙菌斑非常重要。