权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Analysis of functional group distribution of intramembranous globular proteins in plasma membrane of lymphocyte adhesion sites on hydrophilic/ hydrophobic ABA-type microphase-separeted structure surfaces with different lamellar spacings by transmission el

不同片层间距亲疏水ABA型微相分离结构表面淋巴细胞黏附位点质膜膜内球状蛋白功能基团分布分析

基本信息

批准号：
12680844
负责人：
ABE Kazuhiko
金额：
$ 1.22万
依托单位：
TOKYO WOMEN'S MEDICAL UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

The aim of this research was to evaluate the optimum lamellar spacing of PHEMA-PSt-PHEMA ABA-type block copolymer(HSB) surface for bio-compatibility manifestation using lymphocyte with good self/non-self selectional function. The lamellar spacings of the HSB were prepared at 8nm, 16nm and 43nm. PSt and PHEMA-PSt random copolymer(HSR) surfaces were used as controls. Interaction between lymphocytes and polymer surfaces was carried out using Microsphere Column Method. After a fixed incubation time at room temperature, the functional groups of intramembranous globular proteins in the plasma membrane of the lymphocyte adhesion sites on the HSB surfaces were analyzed by Transmission Electron Microscopy(TEM). Then, diameters of the functional groups were quantitatively evaluated, using an image-processor analyzer: especially, the diameters of amino groups(NH_2) of the outer membrane leaflet and the diameters of sulfhydryl groups(SH) of the inner membrane leaflet, in the intaramenbranous globu … More lar proteins. The diameters of the NH_2 and SH in the intramembranous globular proteins of the lymphocyte adhesion sites, on the HSB surfaces of the 16nm-lamellar spacings were the same value as those of the intact lymphocytes. On the other hand, the diameters of the NH_2 and SH in the intramembranous globular proteins of the lymphocyte adhesion sites, on the HSB surfaces of the 8nm and 43nm-lamellar spacings weren't the same value as those of the intact lymphocytes. The NH_2 and SH on the HSB surfaces of the 8nm and 43nm-lamellar spacings were observed to partly cap. Interestingly, while the diameters of the SH on the 8nm-lamellar spacings were the same value as that of the SH on the 43nm-lamellar spacings, the diameters of the NH_2 on the 8nm-lamellar spacings indicated a higher value more than the diameters of the NH2 on the 43nm-lamellar spacings. The functional groups of the lymphocytes adhered to the PSt and HSR surfaces, couldn't be analyzed by TEM due to a necrotic reaction of the lymphocytes. It was indicated that the lamellar spacing size of the HSB surfaces played an important role for the bio-compatibility manifestation. And then, it was suggested that the HSB surfaces with the 16nm-lamellar spacing inhibited the fluidity of the lymphocyte plasma membrane. Less

本研究的目的是评估PHEMA-PSt-PHEMA ABA型嵌段共聚物（HSB）表面的最佳片层间距，以利用具有良好的自我/非自我选择功能的淋巴细胞表现生物相容性。制备的HSB片层间距分别为8 nm、16 nm和43 nm。PSt和PHEMA-PSt无规共聚物（HSR）表面用作对照。淋巴细胞与聚合物表面之间的相互作用采用微球柱法进行。在室温下孵育固定时间后，通过透射电子显微镜（TEM）分析HSB表面上淋巴细胞粘附位点质膜中膜内球状蛋白的官能团。然后用图像分析仪定量测定膜间珠中功能团的直径，特别是外膜小叶的氨基（NH_2）直径和内膜小叶的巯基（SH）直径。 ...更多信息较大蛋白质。淋巴细胞粘附部位的膜内球状蛋白中的NH_2和SH的直径，在16 nm板层间距的HSB表面上与完整淋巴细胞的直径相同。而在8 nm和43 nm片层间距的HSB表面，淋巴细胞粘附部位的膜内球状蛋白中的NH_2和SH的直径与完整淋巴细胞的不同。在片层间距为8 nm和43 nm的HSB表面上观察到NH_2和SH的部分覆盖。有趣的是，虽然8 nm片层间距上的SH的直径与43 nm片层间距上的SH的直径相同，但8 nm片层间距上的NH_2的直径比43 nm片层间距上的NH_2的直径大。淋巴细胞的功能团粘附在PSt和HSR表面，由于淋巴细胞的坏死反应，不能用TEM分析。结果表明，HSB表面的层间距大小对生物相容性的表现起着重要的作用。因此，16 nm片层间距的HSB表面抑制了淋巴细胞质膜的流动性。少