权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

ELUCIDATION OF ANTITHROMBOGENIC MECHANISM ON PHEMA-PST-PHEMA ABA TYPE BLOCK COPOYMER SURFACE WITH LAMELLAR SHAPED-MICRODOMAIN STRUCTURE

层状微域结构PHEMA-PST-PHEMA ABA型嵌段共聚物表面抗血栓形成机制的阐明

基本信息

批准号：
08680936
负责人：
ABE Kazuhiko
金额：
$ 1.54万
依托单位：
TOKYO WOMEN'S MEDICAL COLLEGE
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

项目摘要

In order to elucidate the antithrombogenic mechanism on PHEMA-PSt-PHEMA ABA type block copolymer surfaces (HSB) with lamellar shaped-microdomain structure, ultrastructures of platelet membrane glycocalyx (GC) and membrane skeleton (MS) -dissociated and MS-non-dissociated platelets that adhered to the HSB surfaces were analyzed by transmission electron microscopy (TEM). The number of storage granules per 1mum^2 of MS-non-dissociated and MS-dissociated platelets was then evaluated quantitatively by the image processor-analyzer. PSt and PHEMA-PSt random copolymer (HSR) surfaces were used as controls. The interaction of the platelets and the control polymer surfaces was carried out with the microsphere column method under the different flow rate conditions. The platelet GC was stained with ruthenium red. The dissociation of platelet MS was treated with dibucaine hydrochloride. The platelets on the HSB surfaces adhered with supporting narrow spaces which formed scaffolding, keeping the GC t … More he same as the intact platelet GC.On the other hand, the platelets on the control polymer surfaces adhered without the narrow spaces for the GC,indicating strong adhesion. Their external GC seemed to be lacked in some places. The ultrastructural changes of MS-non-dissociated platelets on the HSB surfaces were inhibited significantly compared to that on the control polymer surfaces. The number of the platelet storage granules on the HSB surfaces did not indicate any significant difference compared to those of the intact platelets. On the other hand, the MS-dissociated platelets adhered to all the control polymer surfaces were observed to form round shapes. The number of the platelet storage granules did not indicate any signifficant difference between all the polymer surfaces and the intact platelets. It was suggested that the microdomain structure of the HSB surfaces inhibited the ultrastructural changes by obstructing the transmembrane signaling to the MS from the GC without regard to the different flow rates. Less

为了阐明PHEMA-PSt-阿坝嵌段共聚物（HSB）表面具有层状微区结构的抗血栓形成机制，用透射电镜（TEM）分析了粘附在HSB表面的血小板膜糖萼（GC）和膜骨架（MS）解离和未解离的超微结构。然后通过图像处理器-分析仪定量评估每1 mum^2 MS-非解离和MS-解离血小板中的储存颗粒数。PSt和PHEMA-PSt无规共聚物（HSR）表面用作对照。在不同流速条件下，用微球柱法研究了血小板与对照聚合物表面的相互作用。血小板GC用钌红染色。用盐酸地布卡因处理血小板MS的解离。HSB表面的血小板粘附在狭窄的支撑空间内，形成脚手架，使GC保持在一定的水平。 ...更多信息另一方面，在对照聚合物表面上的血小板粘附而没有GC的狭窄空间，表明强粘附。他们的外部GC似乎在某些地方缺乏。与对照聚合物表面相比，HSB表面的MS-非解离血小板的超微结构变化被显著抑制。HSB表面的血小板储存颗粒的数量与完整血小板的数量相比没有任何显着差异。另一方面，观察到粘附到所有对照聚合物表面的MS解离的血小板形成圆形。血小板储存颗粒的数量没有表明所有聚合物表面和完整血小板之间的任何显著差异。有人建议，HSB表面的微区结构抑制超微结构的变化，通过阻碍跨膜信号从GC到MS，而不考虑不同的流速。少