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ELUCIDATION OF THE INHIBITED MECHANISM OF IMMUNOCYTE DEATH ON THE SURFACE HYDROPHILIC/HYDROPHOBIC-TYPE MICROPHASE-SEPARATED STRUCTURE

表面亲水/疏水型微相分离结构抑制免疫细胞死亡机制的阐明

基本信息

批准号：
10680804
负责人：
ABE Kazuhiko
金额：
$ 1.02万
依托单位：
TOKYO WOMEN'S MEDICAL UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

Poly (2-hydroxyethyl methacrylate) (PHEMA)-Polystyrene(PSt)-PHEMA ABA-type block copolymer (HSB) surfaces with hydrophilic/hydrophobic-type microhase-separated structure inhibited necrosis of lymphocytes. In contrast, PSt and PHEMA-PSt random copolymer (HSR) surfaces caused necrosis of lymphocytes. In order to clarify the inhibitory mechanism : 1) the glycocalyx of the lymphocytes adhered to and contacted with the HSB surfaces was analyzed by transmission electron microscopy(TEM) ; 2) Neuraminidase-treated lymphocytes adhered to, and contacted with the HSB surfaces were analyzed by scanning electron microscopy(SEM), and TEM respectively. The TEM images of the contacted lymphocytes and the mitochondria were evaluated quantitatively by an image processor / analyzer (IA) ; 3) Dimethyl sulfoxide (DMSO)-treated lymphocytes adhered to the HSB surfaces were analyzed by SEM and TEM. The TEM images of the adhered lymphocytes and the mitochondria were evaluated quantitatively by the IA. Rutheniu … More m red was used to stain the glycocalyx of the lymphocyte plasma membrane. Neuraminidase was used to remove the sialic acid from the glycoproteins of the lymphocyte plasma membrane. DMSO was used to inhibit the surface immunoglobulin capping formation of the lymphocyte plasma membrane. The PSt and HSR surfaces were used as control polymers. The interaction between the polymer surfaces and the lymphocytes was carried out by the microsphere column method. The entire g;lycocalyx of the lymphocytes adhered to and contacted with the HSB surfaces was the same as that of the intact lymphocytes. On analyses of Neuramindase-treated and DMSO-treated lymphocytes, the lymphocytes adhered to and contacted with the polymer surfaces were all observed to be round, keeping cytoplasm well. The computerized TEM image analysis did not indicate significant differences in all cases. It was suggested that the microphase-separated structure surfaces of the HSB inhibited the necrosis of the lymphocytes becasuse the surfaces kept the plasma membrane's structure and function stable. Less

聚甲基丙烯酸羟乙酯（PHEMA）-聚苯乙烯（PSt）-PHEMA ABA型嵌段共聚物（HSB）表面具有亲水/疏水型微孔分离结构，可抑制淋巴细胞的坏死。相反，PSt和PHEMA-PSt无规共聚物（HSR）表面引起淋巴细胞坏死。为了阐明其抑制机制：1）通过透射电子显微镜（TEM）分析粘附和接触HSB表面的淋巴细胞的糖萼; 2）通过扫描电子显微镜（SEM）和TEM分别分析粘附和接触HSB表面的神经氨酸酶处理的淋巴细胞。通过图像处理/分析仪（IA）对接触的淋巴细胞和线粒体的TEM图像进行定量评价; 3）通过SEM和TEM对二甲基亚砜（DMSO）处理的粘附于HSB表面的淋巴细胞进行分析。电镜下观察粘附淋巴细胞和线粒体的形态，并进行定量分析。鲁塞纽 ...更多信息 M红染色淋巴细胞质膜糖萼。神经氨酸酶用于从淋巴细胞质膜的糖蛋白去除唾液酸。DMSO用于抑制淋巴细胞质膜的表面免疫球蛋白帽形成。PSt和HSR表面用作对照聚合物。聚合物表面与淋巴细胞之间的相互作用通过微球柱法进行。与HSB表面粘附和接触的淋巴细胞的整个G;lycocalyx与完整的淋巴细胞相同。对神经氨酸酶处理的和DMSO处理的淋巴细胞进行分析，观察到粘附和接触聚合物表面的淋巴细胞都是圆形的，保持细胞质良好。计算机化TEM图像分析未显示所有病例的显著差异。结果表明，HSB的微相分离结构表面保持了细胞膜结构和功能的稳定，从而抑制了淋巴细胞的坏死。少