A study on the influences of changes of regional tissue blood flow volume on energy metabolism in masticatory muscles

为了阐明慢性血流量减少对咀嚼肌能量代谢的影响，我们尝试建立了用核磁共振获得31P谱的方法，并尝试制作了Wister大鼠腓肠肌和比目鱼肌血流量随优势动脉结扎而慢性减少的模型。用三波长法测定局部组织血流量、血氧饱和度(StO_2)和总血红蛋白(Hb)体积，结果如下：1.利用7T核磁共振仪和直径25 mm的表面线圈，获得了三磷酸腺苷样品的31P谱，并能检测到α，β，γ-三磷酸腺苷的峰。然而，由于周围组织的噪声，大鼠肌肉的~(31)P谱并不清楚。这一问题有望通过对NMR2控制系统程序的改进来解决。利用自制的固定装置，将测量仪的探头和大鼠的腿部同时固定，获得了测量StO2和Hb体积的精确重复性。动脉结扎后，动脉血氧分压和总Hb分别下降约30%和70%。这些水平在绑定后一到两个月持续。因此，可以制作腓肠肌和比目鱼肌血流量明显减少的相对慢性模型。

In order to clarify the influences of chronic decrease in blood flow volume on energy metabolism in masticatory muscles, we tried to establish the method for obtaining 31P-spectra by using NMR, and tried to make the the wister rat model in which the blood flow volume of the gastrocneminus muscles and the soleus muscles chronically continued to decrease with bound dominant artery. As the parameters of regional tissue blood flow volume, oxygen saturation level of blood (StO2) and total hemoglobin (Hb) volume were measured using a three wavelength spectrophotometric method.The results were as follows;1. Using a 7T NMR and a surface coil (25mm diameter), we obtained the 31P-spectra of ATP sample, and we were able to detect peaks of α, β, γ-ATP. However, the 31P-spectra of rat muscles were not obtained clearly because of noise from surrounding tissues. This problem is expected to be solved by improvement in program of control system of the NMR.2. We obtained precise reproduciblity in measurement of StO2 and Hb volume, when we fixed both the probe of measuring apparatus and the leg of rats using devices which we made for fixation appropriately.3. Just after binding of the artery, the value of StO2 and total Hb volume declined by about 30% and 70%, respectively. These levels continued one or two months after binding. Therefore, it was suggested that we could make the relatively chronic model in which the blood flow volume of the gastrocneminus muscle and the soleus muscles decreased obviously.