A study on the influences of changes of regional tissue blood flow volume on energy metabolism in masticatory muscles

为了阐明咀嚼肌血流量慢性减少对咀嚼肌能量代谢的影响，我们尝试建立核磁共振获取31p谱的方法，并尝试制作腓肠肌负肌和比罗鱼肌血流量慢性持续减少并束缚主动脉的大鼠模型。采用三波长分光光度法测定血氧饱和度（StO2）和总血红蛋白（Hb）体积作为局部组织血流量的参数。实验结果如下：1.实验结果表明：利用7T核磁共振仪和直径为25mm的表面线圈，我们获得了ATP样品的31p光谱，我们能够检测到α， β， γ-ATP的峰。然而，由于周围组织的噪声，大鼠肌肉的31p光谱不清晰。通过对nmr - 2控制系统程序的改进，有望解决这一问题。2 .用自制的固定装置将测量仪的探针固定在大鼠的腿上，获得了精确的StO2和Hb体积测量的重复性。动脉刚结合后，StO2值和总Hb体积分别下降约30%和70%。这些水平在绑定后持续一两个月。因此，我们建议可以制作腓肠肌和比目鱼肌血流量明显减少的相对慢性模型。

In order to clarify the influences of chronic decrease in blood flow volume on energy metabolism in masticatory muscles, we tried to establish the method for obtaining 31P-spectra by using NMR, and tried to make the the wister rat model in which the blood flow volume of the gastrocneminus muscles and the soleus muscles chronically continued to decrease with bound dominant artery. As the parameters of regional tissue blood flow volume, oxygen saturation level of blood (StO2) and total hemoglobin (Hb) volume were measured using a three wavelength spectrophotometric method.The results were as follows;1. Using a 7T NMR and a surface coil (25mm diameter), we obtained the 31P-spectra of ATP sample, and we were able to detect peaks of α, β, γ-ATP. However, the 31P-spectra of rat muscles were not obtained clearly because of noise from surrounding tissues. This problem is expected to be solved by improvement in program of control system of the NMR.2. We obtained precise reproduciblity in measurement of StO2 and Hb volume, when we fixed both the probe of measuring apparatus and the leg of rats using devices which we made for fixation appropriately.3. Just after binding of the artery, the value of StO2 and total Hb volume declined by about 30% and 70%, respectively. These levels continued one or two months after binding. Therefore, it was suggested that we could make the relatively chronic model in which the blood flow volume of the gastrocneminus muscle and the soleus muscles decreased obviously.