Study on the pathogenesis of periodontal disease in patients with Down's syndrome

据报道，唐氏综合症患者会伴有严重的牙周病。为了试图了解这背后的潜在机制，我们评估了唐氏综合症患者的中性粒细胞功能。此外，众所周知，患有唐氏综合症S综合征的患者会加速衰老。从过早衰老的角度，我们评估了老年人牙周膜细胞的再生能力。研究结果如下：(1)唐氏综合征患者中性粒细胞对FMLP、C5a和IL-8的趋化作用与年龄匹配的健康人相比没有受到损害。(2)老年人牙周膜细胞的复制寿命比青少年牙周膜细胞短。(3)老年人牙周膜细胞对b-FGFs的趋化反应低于年轻人牙周膜细胞。(4)达到完全衰老的细胞不表达c-fos。(5)尽管迁移细胞表达c-fos，在未迁移的细胞中观察到许多不表达c-fos的细胞，提示c-fos是细胞迁移和细胞增殖所必需的，而c-fos的表达缺失可能是老年人再生能力低下的原因之一。我们得出结论，唐氏综合征患者牙周病的高患病率可能与再生受损而不是中性粒细胞功能低下有关。

Patients with Down's Syndrome have been reported to be accompanied by severe periodontal disease. To try to understand the underlying mechanisms behind this, we assessed neutrophil functions in patients with Down's Syndrome. Additionally, patients with Down ; s Syndrome are known to suffer accelerated aging. From the viewpoint of premature aging, we evaluated regenerative capacity of periodontal ligament cells in aged individuals. The results of the study are as follows.(1) Neutrophil chemotaxis against FMLP,C5a and IL-8 was not impaired in patients with Down's Syndrome when compared with age-matched healthy subjects.(2) Periodontal ligament cells obtained from aged subjects showed shorter replicative life span as compared with those from juvenile donors.(3) Periodontal ligament cells from aged subjects showed less chemotactic responses to b-FGF as compared with those from juvenile donors.(4) Cells reached fully senescence did not express c-fos.(5) Although migrated cells expressed c-fos , there observed many cells which did not express c-fos in unmigrated cells.These results suggest that c-fos is necessary for cell migration as well as cell proliferation, and failure to express c-fos may be one of the reasons for low regenerative capacity seen in aged subjects. We conclude that impaired regeneration rather than low neutrophil function may be involved in the high prevalence of periodontal disease seen in Down's Syndrome patients.