Study on the pathogenesis of periodontal disease in patients with Down's syndrome

唐氏综合症患者牙周病发病机制的研究

• 批准号: 10671966
• 负责人: NISHIMURA Fusanori
• 金额: $ 1.92万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671966/
• 关键词: Down ; s Syndrome; Neutrophil function; Aging; c-fos; Regeneration; c-fos; 組織修復; ダウン症; 好中球機能; 歯根膜組織; 再生能

Patients with Down's Syndrome have been reported to be accompanied by severe periodontal disease. To try to understand the underlying mechanisms behind this, we assessed neutrophil functions in patients with Down's Syndrome. Additionally, patients with Down ; s Syndrome are known to suffer accelerated aging. From the viewpoint of premature aging, we evaluated regenerative capacity of periodontal ligament cells in aged individuals. The results of the study are as follows.(1) Neutrophil chemotaxis against FMLP,C5a and IL-8 was not impaired in patients with Down's Syndrome when compared with age-matched healthy subjects.(2) Periodontal ligament cells obtained from aged subjects showed shorter replicative life span as compared with those from juvenile donors.(3) Periodontal ligament cells from aged subjects showed less chemotactic responses to b-FGF as compared with those from juvenile donors.(4) Cells reached fully senescence did not express c-fos.(5) Although migrated cells expressed c-fos , there observed many cells which did not express c-fos in unmigrated cells.These results suggest that c-fos is necessary for cell migration as well as cell proliferation, and failure to express c-fos may be one of the reasons for low regenerative capacity seen in aged subjects. We conclude that impaired regeneration rather than low neutrophil function may be involved in the high prevalence of periodontal disease seen in Down's Syndrome patients.

据报道，唐氏综合症患者会伴有严重的牙周病。为了试图了解这背后的潜在机制，我们评估了唐氏综合症患者的中性粒细胞功能。此外，众所周知，患有唐氏综合症S综合征的患者会加速衰老。从过早衰老的角度，我们评估了老年人牙周膜细胞的再生能力。研究结果如下：(1)唐氏综合征患者中性粒细胞对FMLP、C5a和IL-8的趋化作用与年龄匹配的健康人相比没有受到损害。(2)老年人牙周膜细胞的复制寿命比青少年牙周膜细胞短。(3)老年人牙周膜细胞对b-FGFs的趋化反应低于年轻人牙周膜细胞。(4)达到完全衰老的细胞不表达c-fos。(5)尽管迁移细胞表达c-fos，在未迁移的细胞中观察到许多不表达c-fos的细胞，提示c-fos是细胞迁移和细胞增殖所必需的，而c-fos的表达缺失可能是老年人再生能力低下的原因之一。我们得出结论，唐氏综合征患者牙周病的高患病率可能与再生受损而不是中性粒细胞功能低下有关。

项目成果

Sawada S, Kokeguchi S, Nishimura F, Takashiba S, Murayama Y: "Phylogenetic characterization of Centipeda periodontii, Selenomonas sputigena and Selenomonas species by 16S rRNA gene sequence analysis."Microbios. 98. 133-140 (1999)

Sawada S、Kokeguchi S、Nishimura F、Takashiba S、Murayama Y：“通过 16S rRNA 基因序列分析对 Centipeda periodontii、Selenomonas sputigena 和 Selenomonas 物种进行系统发育表征。”微生物学。

Nishimura,F.et al.: "Migration inhibitory factor related protein-8 (MRP-8) is an autocrine chemotactic factor for periodoutal ligament cells" J.Dent.Res. in press. (1999)

Nishimura,F.et al.：“迁移抑制因子相关蛋白 8 (MRP-8) 是牙周韧带细胞的自分泌趋化因子”J.Dent.Res。

Sawa,T.,Nishimura,F.et al.: "In vitro induction of activation-induced cell death in lymphocytes from chronic periodontal lesion" Infect.Immun. in press. (1999)

Sawa,T.,Nishimura,F.等人：“体外诱导慢性牙周病灶淋巴细胞中活化诱导的细胞死亡”Infect.Immun。

Sawa, T., Nishimura, F. et al.: "in vitro induction of activation-induced cell death in lymphocytes from chronic periodontal lesion by exogenous Fas-ligand"Infect Immun.. 67. 1450-1454 (1999)

Sawa, T., Nishimura, F. 等人：“通过外源性 Fas-配体在慢性牙周病灶的淋巴细胞中体外诱导活化诱导的细胞死亡”Infect Immun.. 67. 1450-1454 (1999)

Arai, H., Nomura, Y., Kinoshita, M., Nishimura, F., Takigawa, M., Takahashi, K., Washio, N., Takashiba, S., Murayama, Y.: "The inhibition of DNA synthesis by prostaglandin E2 in human gingival fibroblasts is independent of the cyclic AMP-protein kinase A

Arai, H.、Nomura, Y.、Kinoshita, M.、Nishimura, F.、Takikawa, M.、Takahashi, K.、Washio, N.、Takashiba, S.、Murayama, Y.：“DNA 的抑制