Basic studies on possible application of anti-oxidative fraction and constituents of crude drug origin to preventive medicine

抗氧化部位及药材来源成分在预防医学中的可能应用基础研究

• 批准号: 10671998
• 负责人: KINOSHITA Takeshi
• 金额: $ 0.64万
• 依托单位: Teikyo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671998/
• 关键词: licorice; crude drug; anti-oxidative activity; lipid peroxidation preventing activity; superoxide anion; active oxygen species; 活性酸素

1. Chemical investigation was carried out intensively on the methylene chloride extract (PT ext.) of Glycyrrhiza glabra root (Russian licorice) to afford four minor phenolic constituents, two isoflavanones (RL-Q and RL-R), one 3-arylcoumarine (RL-U) and one chromenochromane (RL-V), all of which were undescribed literally, Structures of these compounds were elucidated as 2',4'-dihydroxy-[6",6"-dimethylpyrano(2",3":7,8)]-isoflavanone, [6",6"-dimethylpyrno(2",3":7,8)]-2'-hydroxy,4'-methoxy-isoflavanone, 4',7-dihydroxy-[6",6"-dimethylpyno(2",3":2',3')]-3-arylcoumarin [6',6' -dimethylpyrano(2', 3' : 7, 8)]-benzopyran-3-ol, respectively, on the spectroscopic basis.2. Constituents obtained from Russian licorice described above were tested for anti-microbial activity against gram-positive bacteria including MRSA, yeasts, moulds, and so on. Some of phenolic constituents of this licorice showed potent activity against wide variety of microorganisms.3. The above constituents were assayed for anti … More -oxidative activity on linoleic acid, scavenging activity of superoxide anion (SOィイD3-(/)2ィエD3) generated in both xanthine-xanthine oxidase and microsomal systems, and preventing activity of lipid peroxidation in both microsomal and mitochondrial systems. Many of licorice constituents exhibited outstanding anti-oxidative activity in various assay systems indicating that licorice extract (PT ext.) is a promising agent for the prevention of disease in which active oxygen species are suspected to be implicated on the in-vitro basis. It is of particular interest to note that RL-M showed the most potent anti-oxidative activity among licorice constituents tested though it does not possess catechol moiety that is fond very commonly in naturally occurring antioxidants, Licorice extract also showed significant inhibitory activity against oxidative hemolysis of human erythrocytes, but this assay failed to assess anti-oxidative hemolysis activity of purified constituents due to their direct action to erythrocyte membrane. Less

1.对二氯甲烷提取物（PT ext.）光叶甘草根（俄罗斯甘草），提供四个次要的酚类成分，两个异黄烷酮（RL-Q和RL-R）、一个3-芳基香豆素（RL-U）和一个色烯并色满（RL-V），它们均未在字面上描述。这些化合物的结构被鉴定为2 ′，4 ′-二羟基-[6 ″，6 ″-二甲基吡喃并（2”，3”：7，8）]-异黄烷酮，[6”，6”-二甲基吡喃（2”，3”：7，8）]-2 '-羟基，4'-甲氧基-异黄烷酮，4 '，7-二羟基-[6”，6”-二甲基吡喃酮（2”，3”：2 '，3'）]-3-芳基香豆素[6 '，6' -二甲基吡喃并（2 '，3'：7，8）]-苯并吡喃-3-醇.从俄罗斯甘草中分离得到的酚类化合物对包括MRSA、酵母菌、霉菌等革兰氏阳性菌有较强的抗菌活性，其中部分酚类化合物对多种微生物有较强的抗菌活性.对上述成分进行抗肿瘤活性测定， ...更多信息 - 对亚油酸的氧化活性，对黄嘌呤-黄嘌呤氧化酶和微粒体系统中产生的超氧阴离子（SO_2D_3-（/）2-D_3）的清除活性，以及防止微粒体和线粒体系统中的脂质过氧化的活性。甘草提取物（PT ext.）是一种用于预防疾病的有希望的药剂，其中活性氧类被怀疑在体外基础上涉及。特别感兴趣的是，注意到RL-M在所测试的甘草成分中显示出最有效的抗氧化活性，尽管它不具有在天然存在的抗氧化剂中非常常见的儿茶酚部分。甘草提取物还显示出对人红细胞的氧化性溶血的显著抑制活性，但由于纯化组分对红细胞膜的直接作用，该测定未能评估纯化组分的抗氧化溶血活性。少