Studies on responsiveness of rat retial blood vessels with a newly developed digital funduscope system for small animals

新开发的小动物数字眼底镜系统对大鼠视网膜血管反应性的研究

基本信息

批准号：
10672051
负责人：
ISHII Kunio
金额：
$ 2.11万
依托单位：
Kitasato University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

Retinal arterioles and venules are only central blood vessels that can be observed directly from outside of the body without any surgical procedures. To investigate responsiveness of these blood vessels of the rat, we established a new digital funduscope system that includes a video camera equipped with a 410,000-pixel CCD and a special objective lens for observation of the retina of small animals, such as rats, guinea pigs, rabbits, etc..Male Wistar rats were anesthetized with urethane or sodium thiobutabarbital. Polyethylene cannulae were inserted into femoral artery and femoral vein to monitor arterial pressure (AP) and heart rate and to administer drugs, respectively. Images of the retina were captured by a digital funduscope and recorded on a digital video tape. Changes in diameters of retinal blood vessels (DV) induced by various vasoactive drugs were calculated on a personal computer after making some graphic effects.Nicardipine (1-30μg/kg) lowered AP and increased DV. Nifedipine (10-100μg/kg) lowered AP, but did not affect DV. Flunarizine (1-30μg/kg) did not change AP, but increased DV. Ibudilast (0.1-1.0 mg/kg) and vinpocetine (0.1-1.0 mg/kg) increased DV without any effect on AP.These data demonstrate that changes in DV of rats in response to drugs can be monitored by our newly developed experimental system. Since retinal and cerebral blood vessels are similar in many aspects, our method might be useful for screening of drugs that alter not only retinal but also cerebral circulation.

视网膜小动物和静脉是仅在没有任何手术程序的情况下直接从体外观察到的中央血管。为了调查这些老鼠血管的反应能力，我们建立了一个新的数字底面系统，其中包括配备了410,000像素CCD的摄像机和一个特殊的客观镜头，可观察小动物的视网膜，例如大鼠，豚鼠，兔子等。将聚乙烯套管插入股动脉和股静脉中，以监测动脉压（AP）和心率，并分别服用药物。视网膜的图像由数字底面捕获，并在数字录像带上录制。在产生一些图形效应后，在个人计算机上计算了各种血管活性药物诱导的残留血管（DV）直径的变化。Nicardipine（1-30μg/kg）降低了AP并增加了DV。硝苯地平（10-100μg/kg）降低了AP，但不影响DV。氟苯胺（1-30μg/kg）没有改变AP，而是增加了DV。 ibudilast（0.1-1.0 mg/kg）和长文汀（0.1-1.0 mg/kg）增加了DV，而不会对AP产生任何影响。这些数据表明，我们新开发的实验系统可以监测大鼠DV的变化。由于视网膜和脑血管在许多方面都相似，因此我们的方法可能对筛选不仅改变视网膜和脑循环的药物有用。