Studies on responsiveness of rat retial blood vessels with a newly developed digital funduscope system for small animals

新开发的小动物数字眼底镜系统对大鼠视网膜血管反应性的研究

• 批准号: 10672051
• 负责人: ISHII Kunio
• 金额: $ 2.11万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10672051/
• 关键词: rat; retinal blood vessel; digital funduscope; diameter; CaィイD12+ィエD1 antagonists; ibudilast; cerebral blood vessel; micro circulation; 網膜中心動脈; 網膜中心静脈; デジタル・マイクロスコープ; 眼底カメラ; 糖尿病; Ca^<2+>拮抗薬; 中枢血管

Retinal arterioles and venules are only central blood vessels that can be observed directly from outside of the body without any surgical procedures. To investigate responsiveness of these blood vessels of the rat, we established a new digital funduscope system that includes a video camera equipped with a 410,000-pixel CCD and a special objective lens for observation of the retina of small animals, such as rats, guinea pigs, rabbits, etc..Male Wistar rats were anesthetized with urethane or sodium thiobutabarbital. Polyethylene cannulae were inserted into femoral artery and femoral vein to monitor arterial pressure (AP) and heart rate and to administer drugs, respectively. Images of the retina were captured by a digital funduscope and recorded on a digital video tape. Changes in diameters of retinal blood vessels (DV) induced by various vasoactive drugs were calculated on a personal computer after making some graphic effects.Nicardipine (1-30μg/kg) lowered AP and increased DV. Nifedipine (10-100μg/kg) lowered AP, but did not affect DV. Flunarizine (1-30μg/kg) did not change AP, but increased DV. Ibudilast (0.1-1.0 mg/kg) and vinpocetine (0.1-1.0 mg/kg) increased DV without any effect on AP.These data demonstrate that changes in DV of rats in response to drugs can be monitored by our newly developed experimental system. Since retinal and cerebral blood vessels are similar in many aspects, our method might be useful for screening of drugs that alter not only retinal but also cerebral circulation.

视网膜小动脉和小静脉是可以直接从体外观察到的中枢血管，不需要任何外科手术。为了研究大鼠这些血管的反应性，我们建立了一种新的数字眼底镜系统，该系统包括一个装有41万像素CCD的摄像机和一个用于观察小动物视网膜的专用物镜，如大鼠、豚鼠、兔等。雄性Wistar大鼠用氨基甲酸乙酯或巯丁巴比妥钠麻醉。将聚乙烯套管分别置入股动脉和股静脉，监测动脉压（AP）和心率，并给药。视网膜的图像由数字眼底镜捕获并记录在数字录像带上。在个人电脑上计算各种血管活性药物对视网膜血管直径的影响，并对其进行图形化处理。尼卡地平（1 ~ 30μg/kg）降低AP，增加DV。硝苯地平（10 ~ 100μg/kg）降低AP，但对DV无影响。氟桂利嗪（1 ~ 30μg/kg）不改变AP，但增加了DV。异布司特（0.1 ~ 1.0 mg/kg）和长春西汀（0.1 ~ 1.0 mg/kg）增加了DV，但对ap没有影响。这些数据表明，我们新开发的实验系统可以监测大鼠对药物反应的DV变化。由于视网膜和脑血管在许多方面相似，我们的方法可能有助于筛选既改变视网膜又改变大脑循环的药物。