Development of high-resolution digital fundus camera for small animals and application of it to studies on analysis of responsiveness of retinal blood vessels

小动物高分辨率数码眼底相机的研制及其在视网膜血管反应性分析研究中的应用

基本信息

批准号：
12672116
负责人：
ISHII Kunio
金额：
$ 2.5万
依托单位：
Kitasato University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

We succeeded in developing a high-resolution digital fundus camera for small experimental animals by coupling the objective lens of Scalar VMS-170 digital video camera and the body of Nikon D1 or D1x single-lens reflex digital still camera. A special connecting tube was made to connect both devices, to bring the focal plain of the objective lens just on the CCD with a concave lens and to illuminate the fundus with light guided with glass fibers from a lighting unit. With this camera, we were able to capture clear digital images of rat fundus composed of 2.7- or 5.5-million pixels. Resolving power of the camera was estimated less than 2μm indicating enough capacity for measurement of diameters of rat retinal blood vessels.Responsiveness of rat retinal arterioles and venules to adrenergic agents, angiotensin II, serotonin, nitrovasodilators and calcium antagonists was examined under tetrodotoxin treatment and artificial ventilation. We found that, In streptozotocin-induced diabetic rats, dilator responses to β-adrenergic stimulation of retinal arterioles were greatly impaired, although responses to other agents were little affected.In stroke-prone spontaneously hypertensive rats (SHR-SP), retinal arterioles were narrow by about 30% and dilator responses to nicardipine and fasudil of these blood vessels were augmented. As causes of this phenomenon, enhanced influx of Ca^<2+> and augmented Ca^<2+> sensitivity of vascular smooth muscles due to activation of the Rho kinase in retinal arterioles of SHR-SP were suggested. No apparent change was observed in shape or drug responsiveness in retinal venules of SHR-SP.

我们通过耦合标量VMS-170数码摄像机的物镜和尼康D1或D1X单镜头反射数字摄像机的主体，成功地为小型实验动物开发了高分辨率的数字镜头摄像头。制作了一个特殊的连接管，以连接两个设备，以将物镜的焦点与CCD上的焦镜一起使用凹形镜头，并用带有照明单元的玻璃纤维的光引导来照亮眼底。使用这款相机，我们能够捕获由27.或550万像素组成的大鼠眼底的清晰数字图像。估计摄像机的分辨能力少于2μm，表明足以测量大鼠视网膜血管直径的能力。对肾上腺素，血清素II，血清素，硝基溶剂和钙拮抗剂的响应性在肾上腺素II，血清素蛋白II，血清毒素II和人造毒素治疗下检查。 We found that, In streptozotocin-induced diabetic rats, dilator responses to β-adrenegic stimulation of retinal artiolos were greatly impaired, although responses to other agents were little affected.In stroke-prone spontaneously hypertensive rats (SHR-SP), retinal artiolos were narrow by about 30% and dilator responses to nicardipine and fasudil of these blood vessels were augmented.作为这种现象的原因，提出了Ca^<2+>的增强的影响，并提出了由于Rho激酶在SHR SP-SP的视网膜小动脉中激活而导致血管平滑肌的敏感性增强的敏感性。在SHR-SP的视网膜静脉中，未观察到形状或药物反应性的明显变化。