Elucidation of the mechanism of formation of thermally generated volatile and non-volatile Maillard reaction products using isotope labeling technique combined with ESI-TOF MS/MS and GC/MS analysis.

使用同位素标记技术结合 ESI-TOF MS/MS 和 GC/MS 分析,阐明热产生的挥发性和非挥发性美拉德反应产物的形成机制。

基本信息

  • 批准号:
    RGPIN-2014-03834
  • 负责人:
  • 金额:
    $ 2.19万
  • 依托单位:
  • 依托单位国家:
    加拿大
  • 项目类别:
    Discovery Grants Program - Individual
  • 财政年份:
    2016
  • 资助国家:
    加拿大
  • 起止时间:
    2016-01-01 至 2017-12-31
  • 项目状态:
    已结题

项目摘要

Sugars and amino acids are the key components responsible for the generation of distinctive aroma and color of food products subjected to thermal processing. Many unpleasant-tasting raw foods can be transformed by the interaction of sugars and amino acids (known as Maillard reaction) into desirable products via processes such as bread baking, coffee roasting and chocolate manufacture. Under physiological conditions, many body proteins also can be modified through similar interaction with sugars, specially, in diabetic patients that may result in the impairment of physiological and immunological functions. Unfortunately, this same chemical interaction is also responsible for the thermal generation of food toxicants during cooking such as heterocyclic amines, acrylamide, furan and glycotoxins. Understanding the mechanism by which many of the above mentioned critical food components are formed can contribute to our ability to control their generation. Finding optimal conditions to lower the levels of different toxicants and at the same time retain the Maillard-derived aroma compounds and hence improve the quality and safety of foods, is one of the main challenges of the food industry today. The long term objective of the applicant is to study the origin and the mechanism of formation of volatile and non-volatile compounds formed through the use of model systems. The applicant has developed a convenient method of introducing 13C- and 15N-labeled atoms into the reaction products, through carrying out the reactions with specifically labeled sugars (13C-1, 13C-2, …13C-6) and amino acids (15N, 13C-1, 13C-2 etc) using Pyrolysis-GC/MS as an integrated reaction, separation and identification system. The pyrolysis is conducted between 100-250oC for 20 seconds and requires only sub-milligram quantities of the expensive labeled precursors, thus reducing drastically the cost and time needed to carry out such experiments. Pyrolysis at 250°C for 20 seconds has been shown to mimic heating at 110oC for 1h. One of the advantages of using labeled reactants is that all the C- and N-atoms of a reaction product can be traced back to their origin in the starting material. This can permit elucidation of the mechanism of their formation and structural assignment of their mass spectral fragments. Using the above approach the applicant was able to elucidate the mechanism of formation of various thermally generated volatile and semi-volatile compounds relevant to food such as acrylamide, furan, pyrazines, etc. However, the polar residue remaining after the pyrolysis also contains considerable amount of chemical information in the form of labeled polar products. The short term objectives of the applicant is (1) to analyze these residues in selected model systems for their non-volatile components in addition to the volatiles. The strategy of analyzing the same sample for the volatiles and its residue for non-volatiles allows identification of label incorporation patterns of compounds in both fractions without having to perform additional experiments with costly labeled precursors and to mechanistically link the two pathways. The non-volatile residue can be a rich source of information regarding the origin of color, taste and toxicity due to recently identified link between the levels of consumption of heated foods (fried, grilled, etc.) and chronic diseases associated with aging. (2) To study the influence of selected food matrix components such as phenolics, salts etc on the direction of the Maillard reaction. These parameters have not been studied systematically.
糖和氨基酸是热处理食品产生独特香气和颜色的关键成分。许多味道不好的生食可以通过糖和氨基酸的相互作用(称为美拉德反应)通过面包烘焙、咖啡烘焙和巧克力制造等过程转化为理想的产品。在生理条件下,许多体内蛋白质也可以通过与糖的类似相互作用而被修饰,特别是在糖尿病患者中,这可能导致生理和免疫功能受损。不幸的是,这种化学相互作用也导致烹饪过程中热产生食物毒物,例如杂环胺、丙烯酰胺、呋喃和糖毒素。了解上述许多关键食物成分的形成机制有助于我们控制其产生的能力。寻找最佳条件来降低不同有毒物质的含​​量,同时保留美拉德衍生的香气化合物,从而提高食品的质量和安全性,是当今食品行业面临的主要挑战之一。 申请人的长期目标是研究通过使用模型系统形成的挥发性和非挥发性化合物的起源和形成机制。申请人开发了一种将13C-和15N-标记原子引入到反应产物中的便捷方法,通过使用热解-GC/MS作为集成反应、分离和鉴定系统与特定标记的糖(13C-1、13C-2、…13C-6)和氨基酸(15N、13C-1、13C-2等)进行反应。热解在 100-250oC 之间进行 20 秒,仅需要亚毫克量的昂贵标记前体,从而大大减少了进行此类实验所需的成本和时间。 250°C 下热解 20 秒已被证明可以模拟 110°C 下加热 1 小时。使用标记反应物的优点之一是反应产物的所有 C 和 N 原子都可以追溯到它们在起始材料中的起源。这可以阐明它们的形成机制和它们的质谱片段的结构分配。利用上述方法,申请人能够阐明与食品相关的各种热产生的挥发性和半挥发性化合物(例如丙烯酰胺、呋喃、吡嗪等)的形成机制。然而,热解后剩余的极性残留物还包含大量以标记的极性产物形式存在的化学信息。申请人的短期目标是(1)分析所选模型系统中的这些残留物,除了挥发物之外,还分析其非挥发性成分。分析同一样品中的挥发物及其残留物中的非挥发物的策略可以识别两个组分中化合物的标记掺入模式,而无需使用昂贵的标记前体进行额外的实验并在机械上连接这两个途径。由于最近确定了加热食品(油炸、烧烤等)的消费水平与与衰老相关的慢性疾病之间的联系,非挥发性残留物可以成为有关颜色、味道和毒性来源的丰富信息来源。 (2)研究所选食品基质成分如酚类、盐类等对美拉德反应方向的影响。这些参数尚未得到系统研究。

项目成果

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Yaylayan, Varoujan其他文献

Investigation of various factors for the extraction of peppermint (Mentha piperita L.) leaves
  • DOI:
    10.1016/j.jfoodeng.2009.08.037
  • 发表时间:
    2010-02-01
  • 期刊:
  • 影响因子:
    5.5
  • 作者:
    Dai, Jianming;Orsat, Valerie;Yaylayan, Varoujan
  • 通讯作者:
    Yaylayan, Varoujan
Mechanochemical generation of Schiff bases and Amadori products and utilization of diagnostic MS/MS fragmentation patterns in negative ionization mode for their analysis
  • DOI:
    10.1016/j.carres.2020.108091
  • 发表时间:
    2020-09-01
  • 期刊:
  • 影响因子:
    3.1
  • 作者:
    Xing, Haoran;Yaylayan, Varoujan
  • 通讯作者:
    Yaylayan, Varoujan
Diagnostic MS/MS fragmentation patterns for the discrimination between Schiff bases and their Amadori or Heyns rearrangement products
  • DOI:
    10.1016/j.carres.2020.107985
  • 发表时间:
    2020-05-01
  • 期刊:
  • 影响因子:
    3.1
  • 作者:
    Xing, Haoran;Mossine, Valeri V.;Yaylayan, Varoujan
  • 通讯作者:
    Yaylayan, Varoujan
Thermal degradation of five veterinary and human pharmaceuticals using pyrolysis-GC/MS
Investigation of thermo-chemical properties of mechanochemically generated glucose-histidine Maillard reaction mixtures
  • DOI:
    10.1007/s00217-020-03611-y
  • 发表时间:
    2020-09-16
  • 期刊:
  • 影响因子:
    3.3
  • 作者:
    Xing, Haoran;Yaylayan, Varoujan
  • 通讯作者:
    Yaylayan, Varoujan

Yaylayan, Varoujan的其他文献

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{{ truncateString('Yaylayan, Varoujan', 18)}}的其他基金

Mechanism - guided strategies for the control of the Maillard reaction
机制 - 控制美拉德反应的指导策略
  • 批准号:
    RGPIN-2019-04163
  • 财政年份:
    2022
  • 资助金额:
    $ 2.19万
  • 项目类别:
    Discovery Grants Program - Individual
Mechanism - guided strategies for the control of the Maillard reaction
机制 - 控制美拉德反应的指导策略
  • 批准号:
    RGPIN-2019-04163
  • 财政年份:
    2021
  • 资助金额:
    $ 2.19万
  • 项目类别:
    Discovery Grants Program - Individual
Mechanism - guided strategies for the control of the Maillard reaction
机制 - 控制美拉德反应的指导策略
  • 批准号:
    RGPIN-2019-04163
  • 财政年份:
    2020
  • 资助金额:
    $ 2.19万
  • 项目类别:
    Discovery Grants Program - Individual
Mechanism - guided strategies for the control of the Maillard reaction
机制 - 控制美拉德反应的指导策略
  • 批准号:
    RGPIN-2019-04163
  • 财政年份:
    2019
  • 资助金额:
    $ 2.19万
  • 项目类别:
    Discovery Grants Program - Individual
Elucidation of the mechanism of formation of thermally generated volatile and non-volatile Maillard reaction products using isotope labeling technique combined with ESI-TOF MS/MS and GC/MS analysis.
使用同位素标记技术结合 ESI-TOF MS/MS 和 GC/MS 分析,阐明热产生的挥发性和非挥发性美拉德反应产物的形成机制。
  • 批准号:
    RGPIN-2014-03834
  • 财政年份:
    2018
  • 资助金额:
    $ 2.19万
  • 项目类别:
    Discovery Grants Program - Individual
Elucidation of the mechanism of formation of thermally generated volatile and non-volatile Maillard reaction products using isotope labeling technique combined with ESI-TOF MS/MS and GC/MS analysis.
使用同位素标记技术结合 ESI-TOF MS/MS 和 GC/MS 分析,阐明热产生的挥发性和非挥发性美拉德反应产物的形成机制。
  • 批准号:
    RGPIN-2014-03834
  • 财政年份:
    2017
  • 资助金额:
    $ 2.19万
  • 项目类别:
    Discovery Grants Program - Individual
Elucidation of the mechanism of formation of thermally generated volatile and non-volatile Maillard reaction products using isotope labeling technique combined with ESI-TOF MS/MS and GC/MS analysis.
使用同位素标记技术结合 ESI-TOF MS/MS 和 GC/MS 分析,阐明热产生的挥发性和非挥发性美拉德反应产物的形成机制。
  • 批准号:
    RGPIN-2014-03834
  • 财政年份:
    2015
  • 资助金额:
    $ 2.19万
  • 项目类别:
    Discovery Grants Program - Individual
Elucidation of the mechanism of formation of thermally generated volatile and non-volatile Maillard reaction products using isotope labeling technique combined with ESI-TOF MS/MS and GC/MS analysis.
使用同位素标记技术结合 ESI-TOF MS/MS 和 GC/MS 分析,阐明热产生的挥发性和非挥发性美拉德反应产物的形成机制。
  • 批准号:
    RGPIN-2014-03834
  • 财政年份:
    2014
  • 资助金额:
    $ 2.19万
  • 项目类别:
    Discovery Grants Program - Individual
Isotope labeling studies on the mechanism of Maillard reaction
美拉德反应机理的同位素标记研究
  • 批准号:
    39327-2009
  • 财政年份:
    2013
  • 资助金额:
    $ 2.19万
  • 项目类别:
    Discovery Grants Program - Individual
Isotope labeling studies on the mechanism of Maillard reaction
美拉德反应机理的同位素标记研究
  • 批准号:
    39327-2009
  • 财政年份:
    2012
  • 资助金额:
    $ 2.19万
  • 项目类别:
    Discovery Grants Program - Individual

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