Immunoregulatory function and its mechanism of food components

食物成分的免疫调节功能及其机制

• 批准号: 10680148
• 负责人: AOKI Yosuke
• 金额: $ 2.05万
• 依托单位: Jissen Women's University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10680148/
• 关键词: biophylaxis; medullasin; cathepsin G; granulocytes; NK cell; green tea; 生体防御能; スタノール; プロテアーゼ; 腸管出血性大腸菌; Stx2; NK活性; リンパ球

In order to clarify the effect of food components on immune functions, their effect on neutral proteases such as medullasin and cathepsin G in granulocytes was studied, because both medullasin and cathepsin in granulocytes were shown to be engaged in the regulation of biophylaxis. We found that cathepsin G also enhances natural killer (NK) cell activity by acting directly on NK cells. Addition of docosahexaenoic acid to the diet of mice caused the increase of medullasin activity in granulocytes dose-dependently, to the contrary, saturated fatty acids such as palmitic acid and stearic acid decreased medullasin activity in granulocytes. These results indicate that fatty acid in the diet may influence biophylactic activity. Addition of green tea leaf to the diet of mice decreased both medullasin and cathepsin G activity in granulocytes. To the contrary, green tea extract in the diet of mice increased both protease activities in granulocytes. These results indicate that certain components in green tea increase or decrease these neutral protease levels in granulocytes resulting in the regulation of biophylaxis in vivo.

为了阐明食物成分对免疫功能的影响，研究了它们对粒细胞中的髓质素和组织蛋白酶G等中性蛋白酶的影响，因为粒细胞中的髓质素和组织蛋白酶都被证明参与生物防御的调节。我们发现组织蛋白酶 G 还通过直接作用于 NK 细胞来增强自然杀伤 (NK) 细胞的活性。在小鼠饮食中添加二十二碳六烯酸引起粒细胞中髓质素活性的剂量依赖性增加，相反，饱和脂肪酸如棕榈酸和硬脂酸则降低粒细胞中髓质素活性。这些结果表明饮食中的脂肪酸可能影响生物预防活性。在小鼠饮食中添加绿茶叶可降低粒细胞中髓质素和组织蛋白酶 G 的活性。相反，小鼠饮食中的绿茶提取物增加了粒细胞中的两种蛋白酶活性。这些结果表明绿茶中的某些成分会增加或减少粒细胞中的中性蛋白酶水平，从而调节体内生物防御。

项目成果

T.Yamazaki: "Cathepsin G enhances human natural killer cytotoxicixy"Immunology. 93. 115-121 (1998)

T.Yamazaki：“组织蛋白酶 G 增强人类自然杀伤细胞毒性”免疫学。

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K.Fukai, D.Tsuruta, M.Chanoki, H.Kobayashi, S,Someta, K.Nakagawa, S,Taniguchi, M,Ishii, Y,Aoki.: "Medullasin and inflammatory skin diseases (in Japanese)"Hifu. 40. 122-125 (1998)

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