Microbial degradation of heavy oil that drifted ashore from a Russian tanker, the Nakhodka.

本研究的目的是调查退化和稀释，由结核分枝杆菌sp.H2-5，高分子重量多环芳烃（HPAH）在重型石油中，在福岛的Mikuni-cho冲洗的高处后，从一个被损坏的俄罗斯石油油轮中泄漏，在Nakhodka中去除HPAH，H2 -5被培养在重型石油中，在玻璃珠子上涂抹HPAH，在30 ° C的矿物中，20天。在99%、92%、60%和33%的重油中，苯氮[a]吡啶和苯氮[ghi]吡啶被洗涤了ashore消失的重油中。这些六环芳烃在商用重油（C类）中消失的比例为92%、99%、60%和10%，对H2 - 5中的吡啶降解的甲烷酸酯; H2 - 5中的吡啶降解的甲烷酸酯通过乙酸乙酯提取并由薄层色谱法分析，结果在8个地点。在它们中间，有一个物质在0.19左右的Ry-D2 f-D2值被认为是HPLC、Ms和H-NMR分析中的二羟基酸。其中一个点具有类似的Ryd-D2 f-D2值（大约0.35）到catechol ; However，H-NMR信号与其他信号不同。较新的物质在HPLC中分离了3个峰值，其中2个含有相同的紫外线光谱。3.在洗涤的油中H2 -5的文化变异性，H2 - 5在相同条件下60天内被培养。苯提取物的变异性水平要么是相同的，要么是倾向于与控制因素相比较。自从变异性在同等层中没有找到，变异性在H2-5的治疗中没有被发现。

The purpose of this study is to investigate degradation and elimination, by Mycobacterium sp.H2-5, of high-molecular-weight polycyclic aromatic hydrocarbons (HPAH) in heavy oil, which washed ashore on Mikuni-cho in Fukui Prefecture, after leaking from a wrecked Russian oil tanker, the Nakhodka.1.Degradation of HPAH in the heavy oil.H2-5 was cultivated on the heavy oil, which was coated on glass beads, in mineral medium at 30℃ for 20 days. Benz[a]anthracene, pyrene, benzo[a]pyrene, and benzo[ghi]perylene in the heavy oil that had washed ashore disappeared by 99%, 92%, 60%, and 33%, respectively. These HPAH in commercial heavy oil (class C) disappeared by 92%, 99%, 60%, and 10%, respectively.2.Metabolites in pyrene degradation by H2-5.Meta bolites on pyrene degradation by H2-5 were extracted by ethyl acetate and analyzed by thin layer chromatography, resulting in 8 spots. Among them, the substance having an RィイD2fィエD2 value of around 0.19 was considered to be dihydroxynaphthoic acid from HPLC, Ms, and H-NMR analyses. One of the spots had a similar RィイD2fィエD2 value (around 0.35) to catechol ; however, the H-NMR signals were different from each other. The latter substance was separated into 3 peaks by HPLC, and two of them had the same UV spectra.3.Mutagenicity of the culture of H2-5 on the heavy oil that washed ashore.H2-5 was cultivated for 60 days under the same condition in 1. Mutagenicity levels of the benzene extracts were the same or tended to decrease compared with that of the control. Since mutagenicity was not found in the aqueous layer, increasing of mutagenicity did not occur through the treatment with H2-5.