Microbial degradation of heavy oil that drifted ashore from a Russian tanker, the Nakhodka.

从俄罗斯纳霍德卡号油轮漂流到岸上的重油的微生物降解。

• 批准号: 10680553
• 负责人: MINEKI Shigeru
• 金额: $ 0.38万
• 依托单位: Science University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10680553/
• 关键词: polycyclic aromatic hydrocarbon; Mycobacterium; heavy oil; Nakhodka; metabolite; mutagenicity; Heavy oil; Pyrene; Benzo[a]pyrene; Benz[a]anthracene; Mycobacterium sp.; Bioremediation; Polycyclic aromatic hydrocarbon; Mycobacterium; バイオレメディエー

The purpose of this study is to investigate degradation and elimination, by Mycobacterium sp.H2-5, of high-molecular-weight polycyclic aromatic hydrocarbons (HPAH) in heavy oil, which washed ashore on Mikuni-cho in Fukui Prefecture, after leaking from a wrecked Russian oil tanker, the Nakhodka.1.Degradation of HPAH in the heavy oil.H2-5 was cultivated on the heavy oil, which was coated on glass beads, in mineral medium at 30℃ for 20 days. Benz[a]anthracene, pyrene, benzo[a]pyrene, and benzo[ghi]perylene in the heavy oil that had washed ashore disappeared by 99%, 92%, 60%, and 33%, respectively. These HPAH in commercial heavy oil (class C) disappeared by 92%, 99%, 60%, and 10%, respectively.2.Metabolites in pyrene degradation by H2-5.Meta bolites on pyrene degradation by H2-5 were extracted by ethyl acetate and analyzed by thin layer chromatography, resulting in 8 spots. Among them, the substance having an RィイD2fィエD2 value of around 0.19 was considered to be dihydroxynaphthoic acid from HPLC, Ms, and H-NMR analyses. One of the spots had a similar RィイD2fィエD2 value (around 0.35) to catechol ; however, the H-NMR signals were different from each other. The latter substance was separated into 3 peaks by HPLC, and two of them had the same UV spectra.3.Mutagenicity of the culture of H2-5 on the heavy oil that washed ashore.H2-5 was cultivated for 60 days under the same condition in 1. Mutagenicity levels of the benzene extracts were the same or tended to decrease compared with that of the control. Since mutagenicity was not found in the aqueous layer, increasing of mutagenicity did not occur through the treatment with H2-5.

本研究的目的是研究分支杆菌H2-5对被冲上福井县Mikuni-cho的重油中的高相对分子质量多环芳烃的降解和消除。1.重质油中多环芳烃的降解。H2-5是在30℃的矿物质介质中，包覆在玻璃珠上的重油上培养的。被冲上岸的重油中的苯并[a]菲、芘、苯并[a]芘和苯并[基茂]的消失率分别为99%、92%、60%和33%。这些HPAH在工业重油(C级)中的消失率分别为92%、99%、60%和10%。2.H2-5降解芘的代谢产物用乙酸乙酯提取，用薄层层析分析，得到8个斑点。其中，RィイD2fィエD2值约为0.19的物质经高效液相色谱、质谱分析和核磁共振氢谱分析确定为二羟基萘酸。其中一个斑点的R-ィイD2fィエD2值与邻苯二酚相似(约0.35)，但H-核磁共振信号不同。3.H2-5在洗涤后的重油上的诱变作用。在相同条件下培养60天。1.苯提取物的诱变水平与对照相同或有降低的趋势。由于水层无致突变性，用H2-5处理不会增加致突变性。