PHYSIOLOGICAL ROLE OF NEURONAL-GRIAL INTERACTION

神经元-颗粒相互作用的生理作用

• 批准号: 10680744
• 负责人: INOUE Isao
• 金额: $ 2.11万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10680744/
• 关键词: NEURON; SQUID GAINT AXON; SHWANN CELL; SIGNALING; POTASSIUM CHANNEL; CALCIUM CHANNEL; GLUTAMIC ACID; INTRACELLULAR CALCIUMION; 神経系; 神経細胞; グリア細胞; カリウムホメオスタシス; 不活性化

Schwann cells were enzymatically isolated from squid giant axons. Macroscopic ionic currents were recorded using whole cell clamp and single channel currents using patch clamp. Two types of ionic currents were found with whole cell voltage clamp. One is voltage gated L-type calcium current sensitive to externally applied nifedipine or CoィイD12+ィエD1. This current is activated at >-40 mV. The other current is outwardly rectified potassium current. This current is blocked by externally applied nifedipine or COィイD12+ィエD1, but insensitive to externally applied caffeine, ryanodine or L-glutamate. Single potassium channel currents with a conductance of 40 pS were recorded from excised inside-out patches. The open probability (Po) was 0.05 at 3x10ィイD1-7ィエD1 M CaィイD12+ィエD1, increased transiently to 0.4 when the CaィイD12+ィエD1 concentration was elevated to 1×10ィイD1-6ィエD1 M, then ran down spontaneously at this CaィイD12+ィエD1 concentration. The channel activity was insensitive to externally applied L-glutamate, internally applied acetylcholine (ACh), or ATP. The results suggest that the calcium activated potassium channels play an essential role in generation of the Schwann cell membrane potential of -40 mV, and the membrane potential is controlled by the intracellular CaィイD12+ィエD1 level. Schwann cells were loaded fluo-3, a CaィイD12+ィエD1-sensitive dye. Externally applied K+ induced an increase in the internal CaィイD12+ィエD1 level only when external solution contained CaィイD12+ィエD1. Externally applied L-glutamate, ACh, and caffeine induced no detectable change in the internal CaィイD12+ィエD1level.

从鱿鱼巨型轴突中分离雪旺细胞。使用全细胞夹和单个通道电流使用斑块夹记录宏观离子电流。在全细胞电压夹中发现了两种类型的离子电流。一个是电压门控L型钙电流对外部施用的硝苯地平或COI D12+IE D1敏感。该电流以> -40 mV的速度激活。另一个电流是向外整理的钾电流。该电流被外部施用的硝苯地平或COI D12+IE D1阻塞，但对外部施用的咖啡因，ryanodine或L-谷氨酸不敏感。从出色的内而外的斑块中记录了具有40 ps的单个钾通道电流。当CAI D12+IE D1浓度升高至1×10 D1-6 D1 M时，在3x10i D1-7i D1 M CAI D12+IE D1时，开放概率（PO）为0.05，瞬时增加到0.4，然后在此CAI D12+D1浓度下散发出来。该通道活性对外部施用的L-谷氨酸，内部应用的乙酰胆碱（ACH）或ATP不敏感。结果表明，钙活化的钾通道在-40 mV的schwann细胞膜电位中起着至关重要的作用，并且膜电位受细胞内CAI D12+E D1水平的控制。将Schwann细胞加载Fluo-3，CAI D12+E D1敏感染料。仅当外部溶液包含CAI D12+E D1时，外部应用K+才能诱导内部CAI D12+E D1水平增加。外部施用的L-谷氨酸，ACH和咖啡因诱导内部CAI D12+E D1水平没有可检测到的变化。

项目成果

Inoue, I.: "Evolution of L-type CaィイD12+ィエD1 channel as a voltage sensor in striated muscle."In "CaィイD12+ィエD1 channels, Structure and Phylogeny" Society of Experimental Biology Seminar Series, Cambridge Univ. Press. (in press).

Inoue, I.：“L 型 CaiD12+D1 通道作为横纹肌电压传感器的进化”，《CaiD12+D1 通道、结构和系统发育》实验生物学学会研讨会系列，剑桥大学出版社。 ）。

Tsutsui,I.: "Activation of locomotor and grasping spine muscle fibres in chaetognaths;a curious paradox"J.Muscle Res.Cell Motl.. (in press). (2000)

Tsutsui,I.：“毛颌动物运动和抓握脊柱肌肉纤维的激活；一个奇怪的悖论”J.Muscle Res.Cell Motl..（出版中）。

Tsutsui, I., Inoue, I., Carre, C. & Bone, Q.: "Activation of locomotor and grasping spine muscle fibres in chaetognaths ; a curious paradox. J."Muscle Res. Cell Motl.. (in press). (2000)

筒井，I.，井上，I.，卡雷，C.

Inoue,I.: "The locomotor muscle sheet of Chelophyes and other calycophoran siphonophores:muscle fibres without any sarcoplasmic reticulum but with a T-system analogue"J.mar biol Ass.UK. 79. 1111-1116 (1999)

Inoue,I.：“Chelophyes 和其他管水动物的运动肌片：没有任何肌浆网但具有 T 系统类似物的肌肉纤维”J.mar biol Ass.UK。

Inoue,I.et al: "Effect of lipophilic ions on the intramembrane charge movement and intracellular Ca release in fetal mouse skeletal muscle cells" Jap.J.Physiol.47. 567-570 (1997)

Inoue,I.et al：“亲脂性离子对胎儿小鼠骨骼肌细胞膜内电荷运动和细胞内 Ca 释放的影响”Jap.J.Physiol.47。