Preliminary studies of structure and function of the haptonema.

触体结构和功能的初步研究。

• 批准号: 03454014
• 负责人: INOUE Isao
• 金额: $ 3.07万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03454014/
• 关键词: haptonema; Haphtophyta; Prymnesiophyceae; Chrysochromulina; coiling; CICR; 混合栄養; カルシウムイオン; A23187; カフェイン; プロカイン; ハプト藻綱; 微細藻類; Chysochromulina; 高速ビデオ

The haptonema is a unique cellular organelle known only in the Prymnesiophyceae(=Haptophyceae). Ultrastructue, functional roles and biological significance of this organelle is not well understood. The aim of this study was to establish basic knowledge on the haptonema of both structural and functional aspects. Chrysochromulina was choosen to achieve the project. The following results and conclusions were obtained.Chrysochromulina hirta, the haptonema plays an important role in food capture. Analysis of video-recorded food capturing and phagotrophic processes in C.hirata revealed that : Prey particles adhere and move on the haptonema towards the base ; They are accumulated at a point called particle aggregating center and transformed into tightly packed aggregate ; The aggregate is moved up to the tip of the haptonema ; The haptonema then bends in S-shape, resulting in delivery of the aggregate to the food vacuole. Based on these observations, it is concluded mixotrophy of Chrysochromulina hirta is achieved by integration of various properties of the haptonema including surface adhesion, surface motility, secretion of cement material and bending, which are obviously regulated in chronological order. The haptonema is composed of singlet microtubules arranged in an arc or a circle surronded by haptonemal ER.There components are arranged in asymmetrical fashion and has a single absolute configuration in relation to other cellular components such as the nucleus, food vacuole and the flagellar apparatus. The direction of haptonemal bending and coiling is precisely defined in reltion to its and*

haptonema是一种独特的细胞器，仅存在于prymnesiophyae （= haptophyae）。该细胞器的超微结构、功能作用和生物学意义尚不清楚。本研究的目的是建立在结构和功能方面的haptonema的基本知识。该项目选择了黄墨来完成。得到了以下结果和结论：赤藓属（Chrysochromulina hirta）的色素体在食物捕获中起着重要作用。对平田鸡捕获食物和吞噬过程的录像分析表明：猎物颗粒粘附在附着体上并向基部移动；它们聚集在一个称为粒子聚集中心的点，并转化为紧密堆积的聚集体；聚集体向上移动到附着体的尖端；然后，haptonema弯曲成s形，导致聚集体被输送到食物液泡。综上所述，赤藓的混合营养是由表面黏附、表面运动、水泥物质分泌和弯曲等haptonema的多种特性综合而成的，这些特性明显是按时间顺序调节的。触觉原体由单线态微管组成，微管呈弧形或圆形排列，被触觉原质内质网包围。这些成分以不对称的方式排列，相对于细胞核、食物液泡和鞭毛装置等其他细胞成分具有单一的绝对构型。根据其与*的关系，精确地定义了单音弯曲和卷取的方向

项目成果

Kawachi, M., Inouye, I., Maeda, M.and Chihara, M.: "The haptonema as a food-capturing device : study on Chrysochromulina hirta Prymnesiophyceae)." Phycologia. 30. 563-573 (1991)

Kawachi, M.、Inouye, I.、Maeda, M. 和 Chihara, M.：“触胞体作为食物捕获装置：对 Chrysochromulina hirta Prymnesiophyceae 的研究”。

Kawachi,M.,Inouye,I.,Maeda,O.& Chihara,M.: "The haptonema as a food-capturing device:study on Chrysochromulina hirta(Prymnesiophyceae)." Phycologia. 30. 563-573 (1991)

河内，M.，井上，I.，前田，O.

Inouye, I.and Hori, T.: "High-speed video analysis of the flagellar beat and swimming patterns of algae : probable evolutionary trends in green algae." Protoplasma. 161. 54-69 (1991)

Inouye, I. 和 Hori, T.：“藻类鞭毛节拍和游泳模式的高速视频分析：绿藻可能的进化趋势。”

Inouye, I., Hara, Y.and Chihara, M.: "Fruther observations on Olisthodiscus luteus (Raphidophyceae, Chromophyta) : the flagellar apparatus ultrastructure." Japanese Journal of Phycology. 40. 333-348 (1992)

Inouye, I.、Hara, Y. 和 Chihara, M.：“对 Olisthodiscus uteus（剑藻纲，色藻门）的进一步观察：鞭毛装置超微结构。”

Inouye,I.& Hori,T.: "High-speed video analysis of the flagellar beat and swimming patterns of algae:probable evolutionary trends in green algae." Protoplasma. 161. 54-69 (1991)

井上，I.