MECHANISM OF MAINTENANCE OF POTASSIUM HOMEOSTASIS IN NERVOUS SYSTEM BY GLIAL-NEURONAL INTERACTION

胶质神经元相互作用维持神经系统钾稳态的机制

• 批准号: 03454131
• 负责人: INOUE Isao
• 金额: $ 4.03万
• 依托单位: INSTITUTE FOR ENZYME RESEARCH
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03454131/
• 关键词: Potassium ion; Homeostasis; Glial-neuronal interaction; Squid giant axon; Isolation; Schwann cell; Calcium channel; Calcium activated; Potassium channel; カルシウムイオン; 神経細胞; パッチクランプ法

The K^+ homeostasis was found to be maintained at least three processes. The fast and medium processed were revealed to be due to lateral diffusion and transversal diffusion through microtubular system across the Schwann cell, respectively from the electrophysiological and electron microscopic studies. The slow process was found to be related to ionic mechanism of the Schwann cell by whole cell-patch clamp studies on enzymatically isolated Schwann cells. We found at least three voltage sensitive currents ; inward Ca^<2+> -sensitive outward K^+ current, and inwardly rectifying current that appeared at hyperpolarizing voltages.

发现K^+稳态至少维持了三个过程。从电生理和电镜研究中发现，快速和中等处理分别是由于微管系统在雪旺细胞上的横向扩散和横向扩散。通过对酶分离的雪旺细胞进行全细胞膜片钳研究，发现这一缓慢过程与雪旺细胞的离子机制有关。我们发现至少有三个电压敏感电流；向内Ca^<2+>敏感的向外K^+电流，以及在超极化电压下出现的内向整流电流。

项目成果

Amar, M., Pichon, Y., and Inoue, I: "Patch clamp analysis of the effect of the insectide deltamethrin on insect neurones" J.Exp.Biol.163. 65-84 (1992)

Amar, M.、Pichon, Y. 和 Inoue, I：“昆虫溴氰菊酯对昆虫神经元影响的膜片钳分析”J.Exp.Biol.163。

Wakatsuki, T., Nakaya, Y., and Inoue, I.: "Effect of vasopressin on ATP-sensitive and Ca^<2+> -activated K_+ channels of coronary arterial smooth muscle cells" Am.J.Physiol.263. H491-H496 (1992)

Wakatsuki，T.，Nakaya，Y.，和Inoue，I.：“加压素对冠状动脉平滑肌细胞的ATP敏感和Ca 2 -激活的K_通道的影响”Am.J.Physiol.263。

Inoue, I., Tsusui, I., Bone, Q., and Brown, E.R.: "Evolution of skeletal muscle excitation-contraction coupling and the appearance of dihydropyridine-senstive intramembrane charge movement" Proc.Roy Soc.Lond.B.in press.

Inoue, I.、Tsusui, I.、Bone, Q. 和 Brown, E.R.：“骨骼肌兴奋-收缩耦合的进化和二氢吡啶敏感的膜内电荷运动的出现”Proc.Roy Soc.Lond.B.in

Miyoshi,M.: "Endothelin blocks ATP-sensitive K^+ channel and depolarizes smooth muscle cell of porcine coronary artery" Circulation Res.70. 612-616 (1992)

Miyoshi,M.：“内皮素阻断 ATP 敏感 K^ 通道并使猪冠状动脉的平滑肌细胞去极化”循环 Res.70。

Amar,M.: "Patch clamp analysis of the effect of the insectide deltamethrin on insect neurones" J.Exp.Biol.163. 65-84 (1992)

Amar,M.：“昆虫溴氰菊酯对昆虫神经元影响的膜片钳分析”J.Exp.Biol.163。