Generation of Chlamydia-induced atherosclerosis model mice

衣原体诱导的动脉粥样硬化模型小鼠的产生

• 批准号: 10680774
• 负责人: YAGAMI Kenichi
• 金额: $ 1.73万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10680774/
• 关键词: hypertensive mice; chlamydia pneumoniae; atherosclerosis; Chlamydia pneumoniae; クラミジア; マウス

Pathgenesis of vascular remodeling in hypertensive transgenic mice and infectivity of Chlamydia pneumoniae to mice were examined to generate atherosclerotic disease model mice.1) The hypertensive transgenic mice carrying the human renin and human angiotensinogen gene progressively developed remarkable vascular hypertrophy composed of dedifferentiation of vascular smooth muscule cells and extracellular matrix accumulation in the thoracic aorta. The hyperplasia was predominant in the abdominal aorta. The hypertensive transgenic mice and C57BL/6 control mice fed with atherogenic diet for 3 months were compared in atherosclerotic lesion in aorta. Accelerated atherosclerotic lesion was demonstrated in the hypertensive transgenic mice.2) Infectivity of C. pneumoniae to mice from intranasal route was demonstrated in C57BL/6 mice by detection of antibody and PCR. Infected mice demonstrated no symptom during 3 months after infection. Atherosclerotic lesion was demonstrated in the hypertensive transgenic mice infected with C. pneumoniae.

本研究通过检测高血压转基因小鼠血管重构的发病机制和肺炎衣原体对小鼠的感染性，建立了动脉粥样硬化模型。1）携带人肾素和人血管紧张素原基因的高血压转基因小鼠进行性发生了显著的血管肥大，包括血管平滑肌细胞的去分化和细胞外基质的积聚。腹主动脉以增生为主。用致动脉粥样硬化饲料喂养高血压转基因小鼠和C57 BL/6对照小鼠3个月，比较其主动脉粥样硬化病变。高血压转基因小鼠动脉粥样硬化病变加速。通过抗体检测和PCR检测证实了C57 BL/6小鼠经鼻途径感染肺炎克雷伯菌。感染小鼠在感染后3个月内无症状。高血压转基因小鼠感染C.肺炎。

项目成果

Nishijo N.,Yagami K.et al.: "Vascular remodeling in hypertensive transgenic mice"Exp. Anim.. 48. 203-208 (1999)

Nishijo N.、Yagami K.等人：“高血压转基因小鼠的血管重塑”实验。

Nishijo N.et al.: "Vascular remodeling in hypertensive transgenic mice."Exp Anim. 48(3). 203-208 (1999)

Nishijo N.等人：“高血压转基因小鼠的血管重塑。”Exp Anim。

Nishijo N.,Yagami K.et al.: "Salt-sensitive aortic aneurysm and rupture in hypertensive transgenic mice that overproduce angiotensin II"Lab. Invest.. 78. 1059-1066 (1998)

Nishijo N.、Yagami K.等人：“过量产生血管紧张素 II 的高血压转基因小鼠中的盐敏感性主动脉瘤和破裂”实验室。

Nishijo N., Yagami K et al.: "Vascular remodeling in hypertensive transgenic mice"Exp. Anim.. 48. 203-208 (1999)

Nishijo N.、Yagami K 等人：“高血压转基因小鼠的血管重塑”实验。

Nishijo N., Yagami K et al.: "Salt-sensitive aortic aneurysm and rupture in hypertensive transgenic mice that overproduce angiotensin II"Lab. Invest.. 78. 1059-1066 (1998)

Nishijo N.、Yagami K 等人：“过量产生血管紧张素 II 的高血压转基因小鼠中的盐敏感性主动脉瘤和破裂”实验室。