Modification of host genes in the infected cells with parvovirus

细小病毒感染细胞中宿主基因的修饰

• 批准号: 13680907
• 负责人: YAGAMI Kenichi
• 金额: $ 2.05万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13680907/
• 关键词: parvovirus; rat; thymic lymphoma cell; subtraction; CNTFR_α; アポトーシス; 宿主遺伝子; Tリンパ腫細胞; 遺伝子発現

Rat parvovirus (RPV) propagates in rat thymic lymphoma C58(NT)R cell line, and induces apoptosis. Resistant cells survived after infection reveal different phenotypes such as resistance to apoptosis and tummor suppression compared to parent cell clone. To elucidate the moleculer mechanism of this phenomenon, we tried to identify the genes which express differently between the resistant cells and the parental cell. From the results of representational difference analysis (RDA), 6 known genes and 2 unknown genes were determined. Cathepsin E, thymocyte ARPP and seladin-1 were down-expressed in the parental C58 (NT) cells, and ciliary neurotrophic factor receptor alpha (CNTFR_α), protein associating with small stress protein 1, and envelop gene of Molony murine leukemia virus were enhanced expression in the resistant cell clone, by PCR and Northern blot analysis. These results suggest that enhanced expresson of CNTFR_α contribute to induce resistance to apoptosis in the resistant cell clone.

大鼠细小病毒 (RPV) 在大鼠胸腺淋巴瘤 C58(NT)R 细胞系中繁殖，并诱导细胞凋亡。与亲代细胞克隆相比，感染后存活的耐药细胞表现出不同的表型，例如对细胞凋亡和肿瘤抑制的抵抗力。为了阐明这种现象的分子机制，我们试图鉴定在耐药细胞和亲本细胞之间表达不同的基因。根据代表性差异分析(RDA)的结果，确定了6个已知基因和2个未知基因。 PCR和Northern印迹分析显示，组织蛋白酶E、胸腺细胞ARPP和seladin-1在亲代C58（NT）细胞中表达下调，而睫状神经营养因子受体α（CNTFR_α）、小应激蛋白1相关蛋白和莫洛尼鼠白血病病毒包膜基因在耐药细胞克隆中表达增强。这些结果表明，CNTFR_α 表达的增强有助于诱导耐药细胞克隆对细胞凋亡的抵抗。

项目成果

Ueno et al.: "Propagation of rat parvovirus in thymic lymphoma cell line C58(NT)d and subsequent appearance of a resistant cell clone after lytic infection"Journal of Virology. 75. 3965-3970 (2001)

Ueno 等人：“大鼠细小病毒在胸腺淋巴瘤细胞系 C58(NT)d 中的繁殖以及随后在裂解感染后出现抗性细胞克隆”《病毒学杂志》。

Ueno et al.: "Propagation of rat parvovirus in thymic lymphoma cell line C58(NT)d and subsequent appearance of a resistant cell clone after lytic infection"J.Virology. 75. 3965-3970 (2001)

Ueno 等人：“大鼠细小病毒在胸腺淋巴瘤细胞系 C58(NT)d 中的繁殖以及随后在裂解感染后出现抗性细胞克隆”J.Virology。

Ueno Y. et al.: "Propagation of rat parvovirus in thymic lymphoma cell line C58(NT)d and subsequent appearance of a resistant cell clone after lytic infection"Journal of Virology. 75. 3965-3970 (2001)

Ueno Y.等人：“大鼠细小病毒在胸腺淋巴瘤细胞系C58(NT)d中的繁殖以及随后在裂解性感染后出现抗性细胞克隆”《病毒学杂志》。