Studies on age-dependent resistance of mice to hemagglutinating encephalomyelitis virus infection

小鼠年龄依赖性血凝脑脊髓炎病毒感染抵抗力研究

• 批准号: 62580032
• 负责人: YAGAMI Kenichi
• 金额: $ 0.7万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62580032/
• 关键词: Hemagglutinating encephalomyelitis virus; Coronavirus; mice; Age-dependent resistance; Immunosuppression; Immuno-histochemistry; 酵素抗体法; 年令依存抵抗性; 免疫機能不全マウス; 赤血球凝集性脳脊髄炎ウィルス

The age-dependent resistance of mice to fatal infection with hemagglutinating encephalomyelitis virus (HEV) was investigated. MB-67N strain of HEV caused acute fatal infection of central nervous system(CNS). This virus had neurotropism, and propagated in CNS such as nerve cells of cerebral cortex and Purkinje's cells in cerebellum. In intranasal(in) infection, it was suggested that the virus spread from primary sites of viral replication via offactory nerve to the CNS. Although there was no difference between these infectious process of adult and newborn, age-dependent resistance to fatal encephalomyelitis was found. The resistance of newborn mice was increased progressively accompanied with age, and of age-dependency was more remarkable in intracerebral(ic) than in infection. The resistance in adult mice was influenced with genetic background of mice, but not reduced in immunodeficient (T-, B-, and NK-deficient) and immunosuppressive (prednisolone- and cyclophosphamide-treated)mice. It was suggested that the age-dependent resistance of mice to fatal HEV infection had little relation with maturation of immunological function in viral spread from peripheral site to CNS, but influenced with viral replication on nerve cells in CNS. The factors rented with age-dependent resistance may be production of the interferon in CNS or differentiation and maturation of nerve cells in cerebral cortex.

研究了小鼠对血凝性脑脊髓炎病毒(HEV)致死性感染的年龄依赖性抵抗力。MB-67N株HEV可引起急性致死性中枢神经系统感染。该病毒具有神经嗜性，可在大脑皮层神经细胞和小脑浦肯野氏细胞等中枢神经系统中传播。在鼻内感染中，病毒被认为是从病毒复制的主要部位通过干神经传播到中枢神经系统。虽然成人和新生儿的这些感染过程没有差异，但发现了对致死性脑脊髓炎的年龄依赖性耐药。新生小鼠的抵抗力随年龄的增长而递增，且脑内(Ic)的抵抗力比感染更显著。成年小鼠的抵抗力受小鼠遗传背景的影响，但免疫缺陷(T、B和NK缺陷)和免疫抑制(强的松龙和环磷酰胺治疗)小鼠的抵抗力不会降低。提示小鼠对致死性HEV感染的年龄依赖性抵抗力与病毒外周向中枢神经系统传播过程中免疫功能的成熟程度关系不大，而与中枢神经系统内病毒复制有关。年龄依赖性抵抗的因素可能是中枢神经系统干扰素的产生或大脑皮层神经细胞的分化成熟。