Defence mechanism against Coccidia using common antigens related with host cell invasion

球虫病是由属于顶复门的细胞间原生动物引起的。在初次感染球虫后，宿主获得对再感染的免疫力，并且目前正在努力鉴定能够诱导保护性宿主免疫应答的潜在疫苗抗原。为了鉴定与宿主细胞侵袭有关的艾美耳球虫受体分子，研制了几种抗堆型艾美耳球虫（EA）的鸡单克隆抗体（mAb）。其中6D-12-G10单克隆抗体能识别EA子孢子的锥状抗原，并能显著抑制子孢子对CD 8阳性T淋巴细胞的侵袭。此外，该mAb还显示出与来自密切相关的球虫寄生虫包括新孢子虫和弓形虫的速殖子的交叉反应性。用几种不同的禽艾美耳球虫种的子孢子，包括EA、布氏艾美耳球虫、巨型艾美耳球虫、缓艾美耳球虫、毒害艾美耳球虫、早熟艾美耳球虫和黑艾美耳球虫，测定了其它mAb的种特异性和交叉反应性 ...更多信息 路易斯安那州mAb 8 E-1识别存在于所有艾美耳球虫物种上的共同抗原决定簇，而mAb HE-4、8D-2和5D-11识别种间特异性表位。单克隆抗体8 C-3鉴定了一个种特异性表位。隐孢子虫属（Cryptosporidium）顶复门（Apicomplexa）寄生虫感染是目前公认的导致免疫健康和免疫功能低下的人和动物腹泻的原因。在这项研究中，我们研究了这些鸡单克隆抗体与小鼠和小隐孢子虫的交叉反应性。用间接免疫荧光法检测，6D-12-G10和5D-11分别检测到一个分子量为47.9kDa和154.9kDa的蛋白。单克隆抗体6D-12-G10和HE-4在微小隐孢子虫子孢子上显示强荧光染色。此外，在western bloting，单克隆抗体6D-12-G10确定了一个47.9 kDa的蛋白质的小隐孢子虫。这些结果表明，这些单克隆抗体中的一些可能会识别一个共同的表位上的顶复门寄生虫的顶端复合体。少

Coccidiosis is caused by intercellular protozoa belonging to apicomplexans. After initial infection with Coccidia, hosts acquire immunity to reinfection and much effort is currentry underway to identify potential vaccine antigens capable of inducing protective host immune response. In order to identify potential receptor molecules of Eimeria which are involved in host cell invasion, several chicken monoclonal antibodies (mAbs) against E.acervulina (EA) were developed. One of these mAbs, 6D-12-G10, recognized the conoid antigen of EA sporozoites and significantry inhibited sporozoite invasion of CD8-positive T lymphocytes in vitro. Furthermore, this mAb also showed cross-reactivity with tachyzoites from closely related coccidian parasite including Neospora and Toxoplasma. The species-specificity and cross-reactivity of other mAbs were determined using the sporozoites of several different avian Eimeria species including EA, E.brunetti, E.maxima, E.mitis, E.necatrix, E.praecox and E.tenel … More la. The mAb 8E-1 recognized a common antigenic determinant present on all Eimeria species whereas the mAbs HE-4, 8D-2 and 5D-11 recognized interspeciese-specific epitopes. The mAbs 8C-3 identified a species-specific epitope.The infection of Cryptosporidium parasites belonging to the phylum Apicomplexa is now a well recognized cause diarrhea in immunologically healthy and immunocompromised humans and animals throughout the world. In this study, we studied the cross-reactivity of these chicken mAbs with C.muris and C.Parvum. By indirect immunofluoreacsnt assay, 6D-12-G10 showed an intense fluorescent staining a 47.9 kDa protein, and mAbs 5D-11 identified a 154.9 kDa protein of C.muris. Mabs 6D-12-G10 and HE-4 showed an intense fluorescent staining on C.parvum sporozoites. Furthermore, in western blloting, mAbs 6D-12-G10 identified a 47.9 kDa protein of C.parvum. These results indicate that some of these mAbs may identify a common epitope which represent on the apical complex of the apicomplexan parasites. Less