Control of food poisoning using food and feed additives of chicken monoclonal antibodies.

使用食品和饲料添加剂鸡单克隆抗体控制食物中毒。

• 批准号: 20580357
• 负责人: SASAI Kazumi
• 金额: $ 3万
• 依托单位: Osaka Prefecture University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20580357/
• 关键词: Apicomplexan parasites; リコンビナント抗体; 感染防御; 食品・飼料添加; Apicomplexa; conoid; 鶏型モノクローナル抗体; リコンビナント抗conoid抗体; Cryptosporidium; Eimeria; ニワトリ型モノクローナル抗体; apical complex

In order to protect against Apicomplexan parasites infection, tried to prepare the chicken monoclonal antibodies specific for them. In FY 2008, the cross-reactivity of chicken monoclonal antibodies against Crptosporidium was confirmed. In FY 21, monoclonal antibodies against apical complex which had been reported to be involved in protozoa invasion of host cells had cross-reactivities agains Eimeria spicies, in the 2010 final, ScFv recombinant antibodies were produced and had the cross-reactivities agains Apicomplexan parasites.

为了预防顶复门寄生虫感染，尝试制备针对顶复门寄生虫的特异性鸡单克隆抗体。 2008财年，确认了针对隐孢子虫的鸡单克隆抗体的交叉反应性。 21财年，据报道参与原虫入侵宿主细胞的顶端复合体单克隆抗体与艾美耳球虫有交叉反应，2010年最终生产了ScFv重组抗体，与顶复门寄生虫有交叉反应。

项目成果

Survey and molecular characterization of Cryptosporidium and Giardia spp. in owned companion animal, dogs and cats, in Japan.

隐孢子虫和贾第鞭毛虫属的调查和分子特征。

• 发表时间: 2010
• 期刊: Vet Parasitol
• 影响因子: 2.6
• 作者: Yoshiuchi R;Matsubayashi M;Kimata I;Furuya M;Tani H;Sasai K.
• 通讯作者: Sasai K.

Genetical survey of novel type of Cryptosporidium andersoni in cattle in Japan.

• DOI: 10.1016/j.vetpar.2008.08.021
• 发表时间: 2008-11
• 期刊: Veterinary parasitology
• 影响因子: 2.6
• 作者: M. Matsubayashi;Saki Nagano;T. Kita;T. Narushima;I. Kimata;M. Iseki;Tomoya Hajiri;H. Tani;K. Sasai;E. Baba

Effect of low pH on the morphology and viability of Cryptosporidium andersoni sporozoites and histopathology in the stomachs of infected mice.

低 pH 对安氏隐孢子虫子孢子形态和活力以及感染小鼠胃中组织病理学的影响。

• 发表时间: 2011
• 期刊: Int J Parasitol
• 影响因子: 4
• 作者: Matsubayashi M;Ando H;Kimata I;Takase H;Nakagawa H;Furuya M;Tani H;Sasai K.
• 通讯作者: Sasai K.

Characterization of Monoclonal Antibodies that Recognize the Eimeria tenella Microneme Protein MIC2

• DOI: 10.1645/ge-1558.1
• 发表时间: 2008-12-01
• 期刊: JOURNAL OF PARASITOLOGY
• 影响因子: 1.3
• 作者: Sasai, Kazumi;Fetterer, Raymond H.;Baba, Eiichiroh
• 通讯作者: Baba, Eiichiroh

Molecular Characterization of the Novel Type of Cryptosporidium andersoni Detected in Japan.

日本检测到的新型安氏隐孢子虫的分子特征。

• 发表时间: 2008
• 作者: Matsubayashi M;Nagano S;Kita T;Narushima M;Kimata I;Iseki M;Hajiri M;Tani T;Sasai K;Baba E.
• 通讯作者: Baba E.