消火器癌におけるCXC chemokine hlRH遺伝子の抗腫瘍効果の解析

CXC趋化因子hlRH基因对灭火器癌的抗肿瘤作用分析

• 批准号: 10877196
• 负责人: 渋田 健二
• 金额: $ 1.28万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Exploratory Research
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10877196/
• 关键词: 消火器癌; CXCケモカイン; hlRH

(1)標的遺伝子の選択:癌腫で起こっている遺伝子異常を検出するために、消化器癌手術切除標本ならびに健常組織から拍出したtotalRNAより変換したcDNAを用いてdifferentialdisplay法にて癌組織での発現が著しく増強あるいは低下している遺伝子を検索した。具体的な標的遺伝子として、肝臓癌でmRNAの発現が欠損していたhIRH/SDF-1遺伝子の解析を選訳した。(2)臨床消化器癌での発現量の解析:肝臓癌、食道癌、胃癌、大腸癌の各30症例を対象にRT-PCR法にてRNA発現量を定量的に解析したところ、どの癌腫においても健常部粘膜での発現量と比較して約40%程に低下していたが、癌の臨床病理学的因子との関わりはなく、当該遺伝子の発現低下は癌の生物学的悪性度を筬定するものではなかった。(3)ヒト細胞における増殖抑制効果の検討:多くのヒト癌細胞はレセプターCXCR4を発現しているが、リガンドであるhIRH/SDF-laを発現していないことがわかっているため細胞培養液中に外来性に加えられたhIRHproteinにより細胞増殖抑制が認められるかどうかを現在検討中である。(4)今後の展望:hIRH/SDF-la遺伝子は癌細胞増殖抑制機能を持つ可能性が予想されるため、hIRH/SDF-la遺伝子を各種ヒト癌細胞内に導入し、増殖に対する遺伝子導入効果(増殖抑制効果)の有無を評価する。

The main results are as follows: (1) the child selection of the cancer: the cancer is often detected, the digestive organ cancer is removed by hand, and the normal tissue is photographed by totalRNA. The differentialdisplay method is used to detect the cancer tissue in the cancer tissue. For specific information, please select the mRNA for liver cancer, liver cancer, liver cancer and liver cancer. (2) Analysis of the quantity of cancer of digestive apparatus in bed: 30 cases of liver cancer, 30 cases of esophageal cancer, 30 cases of gastric cancer, 30 cases of large cancer, 30 cases of liver cancer, 30 cases of esophageal cancer, 30 cases of gastric cancer, 30 cases of large cancer, 30 cases of liver cancer, 30 cases of esophageal cancer, 30 cases of gastric cancer, 30 cases of liver cancer, 30 cases of esophageal cancer, 30 cases of liver cancer, 30 cases of esophageal cancer, 30 cases of liver cancer, 30 cases of esophageal cancer, 30 cases of liver cancer, 30 cases of esophageal cancer, 30 cases of liver cancer, 30 cases of esophageal cancer, 30 cases of liver cancer, 30 cases of esophageal cancer, 30 cases of gastric cancer, 30 cases of large cancer, 30 cases of liver cancer, 30 cases of esophageal cancer, 30 cases of gastric cancer, 30 cases of large cancer, 30 cases of liver cancer, 30 cases of esophageal cancer, 30 cases of gastric cancer, 30 cases of liver cancer, 30 cases of esophageal cancer, 30 cases of liver cancer, 30 cases of esophageal cancer, 30 cases of When there is a decrease in the nature of cancer biology, it is necessary to determine the nature of cancer biology. (3) the inhibition of colonization was caused by the addition of extraneous hIRHprotein in the cell culture solution. The inhibition of colonization was not found in the cell culture solution. The results showed that the tumor cells were detected in the CXCR4 cells, and the cells were detected in the cell culture solution. (4) looking forward to the future: the hIRH/SDF-la cancer cell colonization inhibition mechanism can maintain the possibility that all kinds of cancer cells can be introduced into the cells and colonies of all kinds of cancer cells (colonization inhibition results).

项目成果

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Shibuta K,Mori M,et al.: "Reduced expression of the CXC chemokine hlRH/SDF-1 mRNA in hepatoma and digestive tract cancer" Int.J.Cancer. 73. 656-664 (1997)

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