Studies on speciation and dispersal routes of forest insects by molecular analyses

森林昆虫的物种形成和传播途径的分子分析研究

• 批准号: 11306010
• 负责人: KUBOTA Kohei
• 金额: $ 16.31万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11306010/
• 关键词: mitochondrial DNA; forest insect; Carabidae; Cerambicydae; aquatic insect; genetic introgression; hybridization; dispersal route; 分散; クリタマバチ; オオオサムシ亜属; スギカミキリ; ミトコンドリアDNA; ND5; COII

Speciation and dispersal routes of forest insects were studied using analyzes of mitchondrial DNA sequences.At first, ND5 gene of mitochondrial DNA of two related species belonging to Carabidae (Carabus insulicola and C. esakii) analyzed in the border of their distribution ranges. ND5 gene introgressed from C. insulicola to C. esakii irreversibly there, although the two species maintain the parapatric distribution pattern largely. This result suggests the reticulate evolution in this subgenus.The dispersal routes after the last glacial era of Semanotus japonicus (Cerambycidae) were inferred by analyzes of morphological features and COI-COII gene. The hybridization between Anoplophora blabripennis and A. nobilis (Cerambycidae) in Ningxia Hui Autonomous Region, China (Cerambycidae) was recognized by analyzes of morphological features and COII gene. DNA analysis was useful for inferences of evolutionary dynamics of insect pests in this study.Although COII gene of Hydropsyche orientalis (Hydropsychidae) , one of aquatic insects, was amplified by PCR, the sequence of it has not read yet.

利用线粒体DNA序列分析研究了森林昆虫的物种形成和传播途径。首先在Carabus insulicola和C. esakii两种Carabus Carabus Carabus esakii Carabus分布范围的边界处分析了Carabus insulicola和C. esakii Carabus Carabus的线粒体DNA ND5基因。ND5基因在该地区不可逆地从岛螟渗入到岛螟，但两种在很大程度上保持了异居分布格局。这一结果表明该亚属的网状进化。通过形态特征和COI-COII基因分析，推测了天牛科天牛在末次冰期后的传播路线。通过形态特征和COII基因分析，确定了宁夏天牛科牛虻与blabripenanplophora nobilis的杂交关系。在本研究中，DNA分析对害虫进化动力学的推断是有用的。虽然已用PCR扩增出水生昆虫东方水蛉COII基因，但其序列尚未读取。

项目成果

Kubota, K. and Kawata, M.: "Events which occur in the hybrid zones between species belonging to the subgenus Ohomopterus: with analyses of morphology and mitochondrial DNA, and using theoretical model (Coleoptera, Carabidae, genus Carabus)"SHINKA. Vol. 10

Kubota, K. 和 Kawata, M.：“在属于 Ohomopterus 亚属的物种之间的杂交带中发生的事件：通过形态学和线粒体 DNA 分析，并使用理论模型（鞘翅目、步甲科、Carabus 属）”SHINKA。

久保田耕平, 河田雅圭: "オオオサムシ亜属の交雑帯でおこっていること:形態・ミトコンドリアDNA・モデルによるシミュレーションより"SHINKA. Vol.10. 8-13 (2000)

Kohei Kubota，Masakei Kawata：“P. spp. 亚属的杂交区正在发生什么：形态学、线粒体 DNA 和模型模拟” SHINKA Vol. 10. 8-13 (2000)