Effects of Dietary Fiber on Digestive/Absorption Function Assessed by the Ileum Contents Perfusion Method

本研究采用回肠内容物灌注法研究了膳食纤维对人体消化吸收功能的影响。1.测定回肠末端膳食纤维(抗性淀粉和果胶)的值，以评价这些纤维作为膳食纤维的真实价值。2.测定了大鼠摄入膳食纤维(抗性淀粉、果胶、乳果糖和纤维素)后的呼出气体，以评价大肠发酵对热量的摄取。3.呼气试验测定膳食纤维对外源性中性脂肪吸收的影响。结果表明，回肠末端5g果胶的平均采集率为85.0±10.8%。而10g抗性淀粉的平均含量为34.7±9.7%。在这两项研究中，个体之间的差异为20%。因此，果胶或抗性淀粉作为膳食纤维的价值估计分别约为90%和35%。至于纤维在大肠中的发酵，膳食纤维的发酵模式在个体和类型上是不同的。至于纤维对中性脂肪吸收的影响，单次注射5g果胶并不能抑制外源脂肪的吸收。因此，为了在临床环境中降低患者的胆固醇和甘油三酯水平，因此认为有必要更长期地每天服用5g果胶作为膳食纤维的补充。阐明了膳食纤维在人体内的代谢部分及其对消化吸收功能的影响，相信我们的研究结果将会对今后膳食纤维的研究起到推动作用。

In this study, the effects of dietary fiber on digestive/absorption function in human were studied using Ileum Contents Perfusion Method as follows. 1. Measured the values of dietary fiber (resistant starch and pectin) in the terminal ileum to evaluate the true values of these fibers as dietary fiber. 2. In order to evaluate the calorie ingestion by the fermentation in the large intestine, the exhaled gas after dietary fiber (resistant starch, pectin, lactulose and cellulose) ingestion were measured. 3. Effect of dietary fiber on absorption of exogenous neutral fat using breath test. As a result, the mean rate of 5g of pectin collected at the terminal ileum was calculated as 85.0±10.8%. However, the mean rate of 10g of resistant starch was calculated as 34.7±9.7%. Differences among individuals were 20% in both studies. Therefore, the values of pectin or resistant starch as dietary fiber were estimated at about 90% or 35%, respectively. As for fermentation of fiber in the large intestine, patterns of fermentation of dietary fiber were differences among individuals and types of dietary fiber. As for effects of fiber on absorption of neutral fat, a single administration of 5g of pectin did not inhibit the absorption of exogenous lipids. Therefore, in order to reduce the cholesterol and triglyceride levels of patients in a clinical setting, a much longer-term daily administration of 5g of pectin as a DF supplement is thus considered necessary. We can clarify the part of metabolism of dietary fiber and its effects on digestive/absorption function in human body, and we believe that our results stimulate the progress of dietary fiber research in the future.