Neuron-glial interaction in the hypothalamo-neurohypophysial system

下丘脑-神经垂体系统中神经元-胶质细胞的相互作用

• 批准号: 11640663
• 负责人: MIYATA Seiji
• 金额: $ 0.77万
• 依托单位: Kyoto Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11640663/
• 关键词: Glia; Neuorn; Brain; Neurohypophysis; Plasticity; Cell adhesion molecule; Microtubule; Calcium binding protein; バソプレッシン; オキシトシン; 視床下部; 微小管関連蛋白; カルシウム結合蛋白

Magnocellular neurons located in the supraoptic nucleus send their principal axons to terminate in the neurohypophysis, where they release vasopressin and oxytocin into the blood circulation. This magnocellular hypothalamo-neurohypophysial system is known to undergo dehydration and lactation. This structural plasticity is accompanied not only by synaptic remodling, increased direct neuronal membrane apposition, and dendritic bundling in the supraoptic and paraventricular nucleus, but also oraganization of neurovascular contacts in the neurohypophysis. Many molecules that are possibly concerned with dynamic structural remodeling are highly expressed in the hypothalamoneurohypophysial system, although they are generally at low expression levels in other regions of adult brains. Interestingly, some of them are highly expressed only in embryonic brains. On the basis of functions, these molecules are classified mainly into two categories. Cytoskeletal proteins, such as tubulin, microtubule-associated proteins (MAP2d) are responsible for changing both glial and neuronal morphology and location. Cell adhesion molecules, belonginh to immunoglobulin superfamily proteins (Kilon, OBCAM, Thy-1, F3) and extracellular matrix proteins (Phosphacan), also participate in neuronal-glial, neuronal-neuronal, and glial-glial recognition and guidance.

位于视上核的大细胞神经元发出其主要轴突终止于神经垂体，在那里它们释放加压素和催产素进入血液循环。这种大细胞下丘脑-神经垂体系统是已知的经历脱水和泌乳。这种结构的可塑性不仅伴随着突触重塑，增加直接神经元膜附着，和树突束在视上核和室旁核，但也oraganization的神经血管接触的神经垂体。许多可能与动态结构重塑有关的分子在下丘脑神经垂体系统中高度表达，尽管它们在成人大脑的其他区域中通常处于低表达水平。有趣的是，其中一些只在胚胎大脑中高度表达。根据功能，这些分子主要分为两类。细胞骨架蛋白，如微管蛋白、微管相关蛋白（MAP 2d）负责改变胶质细胞和神经元的形态和位置。细胞粘附分子，免疫球蛋白超家族蛋白（Kilon，OBCAM，Thy-1，F3）和细胞外基质蛋白（Phosphacan），也参与神经元-神经胶质，神经元-神经元和神经胶质-神经胶质的识别和指导。

项目成果

Miyata, S., Takamatsu, H. et al.: "Plasticity of neurohypophysial terminals with increased hormonal release during dehydration : ultrastructural and biochemical analyses"Journal of Comparative Neurology. 434. 413-427 (2001)

Miyata, S., Takamatsu, H.等人：“脱水过程中激素释放增加的神经垂体末梢的可塑性：超微结构和生化分析”比较神经病学杂志。

Miyata et al.,: "Expression of the IgLON cell adhesion molecules Kilon and OBCAM in hypothalamic magnocellular neurons"Journal of Comparative Neurology. Vol.242. P.74-85 (2000)

Miyata 等人：“下丘脑大细胞神经元中 IgLON 细胞粘附分子 Kilon 和 OBCAM 的表达”比较神经病学杂志。

Matsunaga et al.,: "Expression of high level of tubulin and microtubule-associated protein-2D in the neurohypophysial astrocytes of adult rat"Neuroscience. (印刷中).

Matsunaga 等人：“成年大鼠神经垂体星形胶质细胞中高水平微管蛋白和微管相关蛋白 2D 的表达”神经科学（正在出版）。

Miyata, S.: "Calbindin-D28k and calretinin in the rat neurohypophysis; light and electronmicroscopic localization and up-regulation with dehydration"Journal of Neurocytology. (印刷中). (2000)

Miyata, S.：“大鼠神经垂体中的钙结合蛋白-D28k 和钙结合蛋白；脱水时的光和电子显微镜定位和上调”《神经细胞学杂志》（2000 年出版）。

Miyata S.: "Morphological plasticity and rearrangement of cytoskeletons in pituicytes cultured from adult rat neurohypophysis."Neuroscience Research. 33. 299-306 (1999)

Miyata S.：“从成年大鼠神经垂体培养的垂体细胞中细胞骨架的形态可塑性和重排。”神经科学研究。