Activity-dependent regulation of the expression of chondroitin sulfate proteoglycan and neuronal plasticity

硫酸软骨素蛋白聚糖表达和神经元可塑性的活性依赖性调节

• 批准号: 16500218
• 负责人: MIYATA Seiji
• 金额: $ 2.37万
• 依托单位: Kyoto Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16500218/
• 关键词: Proteoglycan; Neuron; Plasticity; tPA; Phosphacan; Neurocan; Vasopressin; Perineuronal nets; コンドロイチン硫酸プロテオグリカン; オキシトシン; 脳; Phosphacan; 細胞外マトリクス

Chondroitin sulfate proteoglycans (CSPGs) are glycoproteins that carry covalently bound sulfated glycosaminoglycan chains, and more than 30 primary structures of core proteins have been elucidated. CSPGs are main components of extracellular matrix in the central nervous system. The present research program demonstrated following main results.1) The levels of phosphacan proteoglycan was regulated with activity-dependent manner and this downregulation was mediated via proteolysis by tPA action.2) Phosphacan was localized at axonal membrane of parallel fibers in the molecular layer and inhibited adhesion and extension of granule cells and promoted the fasciculation of parallel fibers.3) Cultured cortical neurons were able to construct perineuronal net-like structure without glial cells. This result indicate that perineuronal net is neuronal origin.

硫酸软骨素蛋白多糖(CSPGs)是一种携带共价结合糖胺多糖链的糖蛋白，已有30多种核心蛋白的一级结构被阐明。CSPG是中枢神经系统细胞外基质的主要成分。本研究的主要结果如下：1)磷酸多糖蛋白多糖水平的调节依赖于活性，这种下调是通过tPA作用介导的蛋白水解性的。2)磷酸多糖定位于分子层平行纤维的轴膜上，抑制颗粒细胞的黏附和伸展，促进平行纤维的成束。3)培养的皮质神经元能够在没有胶质细胞的情况下形成神经周网状结构。这一结果表明，周围神经网络是神经元起源的。

项目成果

Matrix-degrading enzymes tissue plasmino activator and matrix metalloprotease-3 in hypothalamo-neurohypophysial system.

下丘脑神经垂体系统中的基质降解酶组织纤溶酶激活剂和基质金属蛋白酶 3。

• 发表时间: 2005
• 期刊: Brain Research 1058
• 作者: Miyata;S.;Nakatani;Y.;Hayashi;N;Nakashima T
• 通讯作者: Nakashima T

Neuronal expression of the chondroitin sulfate proteoglycans phosphacan and receptor-type protein-tyrosine phosphates β.

硫酸软骨素蛋白聚糖磷酸聚糖和受体型蛋白酪氨酸磷酸β的神经元表达。

• 发表时间: 2005
• 期刊: Neuroscience 131
• 作者: Hayashi;N.;Miyata;S.;Yamada;M.;Kamei;K.;Oohira;A.
• 通讯作者: A.

Activity-dependent regulation of a chondroitin sulfate proteoglycan 6B4 phosphacan/RPTPβ in the hypothalamo-neurohypophysial system

下丘脑-神经垂体系统中硫酸软骨素蛋白聚糖 6B4 磷酸聚糖/RPTPβ 的活性依赖性调节

• 发表时间: 2004
• 期刊: Brain Research 1017
• 作者: Miyata;S.;Akagi;A.;Hayashi;N.;Watanabe;K.;Oohira;A.
• 通讯作者: A.

Immobilization of diverse foreign proteins in viral polyhedra and potential application for protein microarrays

• DOI: 10.1002/pmic.200500022
• 发表时间: 2006-01-01
• 期刊: PROTEOMICS
• 影响因子: 3.4
• 作者: Ikeda, K;Nakazawa, H;Mori, H
• 通讯作者: Mori, H

Localization of receptor-type protein tyrosine phosphataseβ in the cerebral and hippocampal neurons of adult rats

受体型蛋白酪氨酸磷酸酶β在成年大鼠大脑和海马神经元中的定位

• 发表时间: 2005
• 期刊: Brain Research 1050
• 作者: Hayashi;N.;Oohira;A.;Miyata;S.
• 通讯作者: S.