Basic study on mass production of therapeutic proteins for serious disease by genetically engineered rice cell

点击翻译按钮获取中文摘要

The following significant results were obtained from the research on production of therapeutic protein, α_1-antitrypsin (AAT) by genetically engineered rice cell.(1) Growth rate of genetically engineered cell was the same as that of non-transformed rice cell. Specific growth rate of rice cell, 0.3 d^<-1>, was of the same order of magnitude as those of other plant cells.(2) Oxygen transfer coefficient K_La was 7-8 h^<-1>, and aeration rate did not affect the growth rate of rice cell.(3) Ammonium was produced at cell growth phase and AAT production phase. However, ammonium did not affect the AAT productivity.(4) Adjustment of osmotic pressure between growth medium and production medium has favorable effective for AAT production.(5) AAT productivity was tripled using pyrvic acid as an altemative carbon source.(6) Continuous production was achieved in the continuous stirred tank reactor.(7) Cell density was increased up to 14 g-dry cell/L in the batch-type reactor.(8) Since maximum productivity of AAT at optimal condition was 25 mg/g-dry cell, at least 350 mg/L of AAT can be produced in batch-type reactor.The recombinant proteins can be rapidly induced by sugar depletion in the employed expression system. Our research strongly suggests that protein production system more efficient than microbial system can be constructed by the rice cell culture system. We hope the mass production system using plant cell culture can be achieved by exploring process-engineering research based on this fundamental research.