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Genetic linkage analysis of ecological traits and molecular markers in Lilium

百合生态性状及分子标记遗传连锁分析

基本信息

批准号：
11660033
负责人：
HIRAMATSU Michikazu
金额：
$ 2.3万
依托单位：
Kyushu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11660033/
关键词：
Lily Lilium longiflorum Lilium formosanum ecological trait genetic analysis genetic linkage map

项目摘要

The aim of the present research is to reveal evolutionary and genetic backgrounds of ecological traits that significantly differentiate closely- related, and -distributed species, Lilium longiflorum and L. formosanum.Population genetic analysis based on allozyme variations suggests that L. formosanum is a derivative from southern peripheral populations of L. longiflorum around the late Pleistcene. The `early flowering` trait characterized by virgin flowering coming within a year from sowing in L. formosanum seemed to be reflected in the significantly larger annual net production in a plant body above the ground than that of L. longiflorum, even in the case that the annual net production in an underground part was comparable between the species.All F_1 progenies generated from crosses between L. formosanum and L. longiflorum flowered within a year from sowing. Self-compatibility and summer flowering traits of L. formosanum appeared almost dominantly in F_1 progenies. These results indicate that accumulated dominant mutations for such ecological traits in an ancestral population promote speciation of L. formosanum from L. longiflorum.Progenies in F_2 were segregated into ones with or without the `early-flowering` trait originated from L. formosanum. The `early-flowering` was appeared in individuals with the larger net production. This indicates that genes controlling net production closely concern the `early flowering`.Construction of genetic linkage map with randomly amplified polymorphic DNA (RAPD) markers was quite difficult, since stably-resolved RAPD markers were not obtained sufficiently. Thus, mapping of molecular markers and Quantitative trait loci (QTL) should be conducted using such alternative stably-resolved DNA markers as amplified fragment length polymorphism (AFLP).

本研究的目的是揭示显着区分密切相关且分布的物种长花百合和台湾百合的生态性状的进化和遗传背景。基于等位酶变异的群体遗传分析表明，台湾百合是晚更新世左右长花百合南部外围种群的衍生物。台湾乳香以播种后一年内开花为特征的“早花”性状似乎反映在地上部分的年净产量显着高于长香，即使在地下部分的年净产量在物种之间具有可比性。台湾乳香和长香的杂交产生的所有 F_1 后代均在播种后一年内开花。台湾乳香的自交亲和性和夏季开花性状几乎主要出现在F_1后代中。这些结果表明，祖先种群中这些生态性状的显性突变累积促进了长花乳香的物种形成。F_2的后代被分离为具有或不具有来自台湾乳香的“早花”性状的后代。 “早花”出现在净产量较大的个体上。这表明控制净产量的基因与“早花”密切相关。利用随机扩增多态性DNA（RAPD）标记构建遗传连锁图谱相当困难，因为没有充分获得稳定解析的RAPD标记。因此，分子标记和数量性状基因座（QTL）的作图应使用诸如扩增片段长度多态性（AFLP）等替代性稳定解析的DNA标记来进行。