Genetic linkage analysis of ecological traits and molecular markers in Lilium

本研究的目的是揭示近缘分布种麝香百合和百合生态性状的进化和遗传背景。基于等位酶变异的群体遗传学分析表明，L. formosanum是L. longiflorum在晚更新世左右。早花性状的特点是在L. Formosanum的地上部年净生产量明显高于L. longiflorum的地下部分年净生产量在种间相当的情况下也是如此。formosanum和L.麝香草在播种后一年内开花。自交亲和性与夏花期性状。在F_1代中，台湾根瘤菌几乎占绝对优势。这些结果表明，在祖先群体中积累的这些生态性状的显性突变促进了L。formosanum，L. F_2代分离出具有和不具有起源于L. longiflorum的“早花”性状的后代。福尔摩沙净生产量大的个体出现“早花”现象。这表明，控制净产量的基因与“早开花”密切相关。由于没有获得足够的稳定的RAPD标记，利用RAPD标记构建遗传连锁图谱十分困难。因此，分子标记和数量性状基因座（QTL）的定位应该使用诸如扩增片段长度多态性（AFLP）等替代的稳定解析的DNA标记进行。

The aim of the present research is to reveal evolutionary and genetic backgrounds of ecological traits that significantly differentiate closely- related, and -distributed species, Lilium longiflorum and L. formosanum.Population genetic analysis based on allozyme variations suggests that L. formosanum is a derivative from southern peripheral populations of L. longiflorum around the late Pleistcene. The `early flowering` trait characterized by virgin flowering coming within a year from sowing in L. formosanum seemed to be reflected in the significantly larger annual net production in a plant body above the ground than that of L. longiflorum, even in the case that the annual net production in an underground part was comparable between the species.All F_1 progenies generated from crosses between L. formosanum and L. longiflorum flowered within a year from sowing. Self-compatibility and summer flowering traits of L. formosanum appeared almost dominantly in F_1 progenies. These results indicate that accumulated dominant mutations for such ecological traits in an ancestral population promote speciation of L. formosanum from L. longiflorum.Progenies in F_2 were segregated into ones with or without the `early-flowering` trait originated from L. formosanum. The `early-flowering` was appeared in individuals with the larger net production. This indicates that genes controlling net production closely concern the `early flowering`.Construction of genetic linkage map with randomly amplified polymorphic DNA (RAPD) markers was quite difficult, since stably-resolved RAPD markers were not obtained sufficiently. Thus, mapping of molecular markers and Quantitative trait loci (QTL) should be conducted using such alternative stably-resolved DNA markers as amplified fragment length polymorphism (AFLP).