Genetic world and Japanese map of plant viruses

遗传世界和日本植物病毒图谱

• 批准号: 11660051
• 负责人: OHSHIMA Kazusato
• 金额: $ 2.24万
• 依托单位: Saga University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11660051/
• 关键词: Turnip mosaic virus; Potato virus Y; Phylogenetic tree; Molecular evolution; Recombinant; 分子造化

Turnip mosaic virus (TuMV), a species of the genus Potyvirus, occurs worldwide. Seventy six isolates of TuMV were collected from around the world, mostly from Brassica and Raphanus crops, but also several non-brassicaceous species. Host tests grouped the isolates into one or other of two pathotypes. The nt sequences of the first protein (P1) and coat protein (CP) genes of their genomes were determined. The phylogenetic relationships of these sequences were assessed separately. The taxonomies of the two genes were similar, and revealed two broadly consistent host-specific lineages among the isolates, and also clear geographic clustering ; one lineage was mostly from Raphanus crops in East Asia, the other was genetically and geographically much more diverse and came from Brassica crops and non-brassicaeous species. Further comparisons showed that many of the isolates were recombinants, some multiply and most intragenically. They also showed that the most consistent phylogenetic signal delimiting the Raphanus lineage came from the 3' terminal half of the P1 gene and the 5' terminal half of the CP gene. The phylogenetic relationships indicate that genomic recombination and host adaptation have occurred during migratory spread of the virus, perhaps from the Mediterranean region and Europe to the other parts of the world, and that the Raphanus pathotype lineage has recently evolved in East Asia. This is probably the most detailed study yet on the origins, worldwide migratory spread, host adaptation, pathogenic segregation and genetic recombination of a single plant virus species currently causing a world crop pandemic. Moreover, molecular evolution of Potato virus Y necrotic strain in Japan was investigated.

芜菁花叶病毒（TuMV）是芜菁花叶病毒属的一种，分布在世界各地。从世界各地收集到76株TuMV分离株，主要来自芸苔属和莴苣属作物，也有一些非芸苔属植物。宿主试验将分离物分为两种病型中的一种或另一种。测定了它们基因组中第一蛋白（P1）和外壳蛋白（CP）基因的nt序列。分别评价了这些序列的系统发育关系。两个基因的分类相似，在分离物中显示了两个大致一致的宿主特异性谱系，并且具有明显的地理聚类；一个谱系主要来自东亚的莴苣属作物，另一个谱系在遗传和地理上更加多样化，来自芸苔属作物和非芸苔属物种。进一步的比较表明，许多分离株是重组的，有些是繁殖的，大多数是内生的。他们还表明，划定Raphanus谱系的最一致的系统发育信号来自P1基因的3‘端和CP基因的5’端。系统发育关系表明，在病毒迁移传播期间（可能从地中海地区和欧洲到世界其他地区）发生了基因组重组和宿主适应，并且Raphanus病型谱系最近在东亚进化。这可能是迄今为止对目前引起世界作物大流行的单一植物病毒物种的起源、全球迁移传播、宿主适应、病原分离和遗传重组进行的最详细的研究。此外，还对日本马铃薯Y病毒坏死株的分子进化进行了研究。

项目成果

A.K.Kundu: "Cross-reactive and major virus-specific epitopes are located at the N-terminal halves of the cylindrical inclusion proteins of TuMV and ZYMV"Archives of Virology. (in press). (2000)

A.K.Kundu：“交叉反应性和主要病毒特异性表位位于 TuMV 和 ZYMV 圆柱形包涵蛋白的 N 端半部”病毒学档案。

K.Oshima: "Accumlation of helper component/proteinase and coat protein of turnip mosaic virus in intact plants"Acta Virologica. 43. 31-37 (1999)

K.Oshima：“萝卜花叶病毒的辅助成分/蛋白酶和外壳蛋白在完整植物中的积累”《病毒学报》。

Ohshima,K. et al: "Potato tuber necrotic ringspot Disease occurring in Japan : Its association with Potato virus Y necrotic strain"Plant Disease. 84. 1109-1115 (2000)

大岛，K.

Chshima,K. et al.: "Potato tuber necrotic ringspot disease occurring in Japan : Its association with Potato virus Y necrotic strain"Plant Disease. 84. 1109-1115 (2000)

奇岛，K.

Ohshima, K.et al.: "Potato tuber necrotic ringspot disease occurring in Japan : Its association with Potato virus Y necrotic strain"Plant Disease. Vol.84. 1109-1115 (2000)

Ohshima, K.等人：“日本发生的马铃薯块茎坏死环斑病：与马铃薯病毒 Y 坏死株的关联”植物病害。