Molecular Histo-cytochemical Analysis of Gene Transcription Factors Reflecting in the Regulation of Functional Differentiation of the Cell

反映细胞功能分化调节的基因转录因子的分子组织细胞化学分析

基本信息

  • 批准号:
    11670014
  • 负责人:
  • 金额:
    $ 2.24万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    1999
  • 资助国家:
    日本
  • 起止时间:
    1999 至 2001
  • 项目状态:
    已结题

项目摘要

1. During the gestation, the ehorionie cells of placentas differentiate into the villous cells which express the prolactin family protein gene. To clarify whether difference of cellular distribution of a transcription factor, Pit-1, which enhances expression of the gene reflects the state of the differentiating cells, we performed Southwestern histochemistry of which method localizes DNA-binding transcription factors. In rat placentas at the late gestational days, the villous cells possessing intranuclear Pit-1 seemed to be identical to the villous cells expressing the prolactin family protein genes in their cytoplasm.2. To understand further the relationship between nuclear components and intranuclear state of transcription factors in differentiating cells, Southwestern histochemistry was performed at ultrastruetural level. The DNA-binding Pit 1 was localized in the euchroraatin of nuclei both of the chorionic villous cells which express prolactin family protein genes and of anterior … More pituitary cells which express prolactin gene. It is suggested that the localization of DNA-binding transcription factors in euchromatin in which the active chromatins are located would be essential for functional differentiation of the cells.3. To analyze a relationship of intranuclear sites between DNA-binding transcription factors and the genomic DNAs or the transcripts, as a model, two different nucleic acids were ultrastructurally localized. Since 45S rRNA is stoichyometrically spliced to 18S rRNA and 28S rRNA, we examined simultaneous localization of both rRNAs in mouse testicular tissue sections using different sized colloidal gold particles in post-embedding method of in situ hybridization. Electron microscopic in situ hybridization revealed that different distribution between these rRNAs was seen in the nucleoli of Sertoli cells. Our findings suggest that the mutual distribution of nuclear substances with different metabolisms, such as the rRNAs as well as the transcription factors and the transcripts, would be quantitatively demonstrated in the nuclei by the method. Less
1. 在妊娠期,胎盘的毛囊细胞分化为表达催乳素家族蛋白基因的绒毛细胞。为了弄清增强基因表达的转录因子Pit-1的细胞分布差异是否反映了分化细胞的状态,我们进行了西南组织化学,该方法定位了dna结合转录因子。在妊娠后期的大鼠胎盘中,具有核内Pit-1的绒毛细胞似乎与细胞质中表达催乳素家族蛋白基因的绒毛细胞相同。为了进一步了解分化细胞中核成分与转录因子核内状态的关系,我们在超微结构水平上进行了西南组织化学。dna结合坑1定位于表达催乳素家族蛋白基因的绒毛膜绒毛细胞和表达催乳素基因的垂体前叶细胞的细胞核的正染色质上。这表明,dna结合转录因子在常染色质中的定位对细胞的功能分化至关重要。为了分析dna结合转录因子与基因组dna或转录本之间的核内位点关系,作为模型,对两种不同的核酸进行了超微结构定位。由于45S rRNA与18S rRNA和28S rRNA进行了化学对称剪接,我们使用不同大小的胶体金颗粒在原位杂交的后包埋方法中检测了这两种rRNA在小鼠睾丸组织切片中的同时定位。电镜原位杂交显示,这些rna在支持细胞核仁中的分布存在差异。我们的研究结果表明,通过该方法可以定量地显示核中具有不同代谢的核物质,如rrna以及转录因子和转录本的相互分布。少

项目成果

期刊论文数量(27)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Shin-ichi Izumi, et al: "In situ hybridization : Electron microscopy In : Histo-cytochemistry 1999. Japan society of Histochemistry and Cytochemistry ed. (in Japanese)"Gakusai Kikaku. 136-141 (1999)
Shin-ichi Izumi 等:“原位杂交:电子显微镜:组织细胞化学 1999 年。日本组织化学和细胞化学学会编辑(日语)”Gakusai Kikaku。
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    0
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和泉伸一 他: "免疫組織化学およびサウスウェスタン組織化学の電顕技術"医学生物学電子顕微鏡技術学会誌. 15(1). 64-69 (2000)
Shinichi Izumi 等人:“免疫组织化学和西南组织化学的电子显微镜技术”医学生物学和电子显微镜技术学会杂志 15(1) (2000)。
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    0
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和泉伸一 他: "反射電顕の分子組織細胞化学への応用"医学生物学電子顕微鏡技術学会誌. 16(1). 46-48 (2001)
Shinichi Izumi 等人:“反射电子显微镜在分子组织和细胞化学中的应用”医学生物学和电子显微镜技术学会杂志 16(1) (2001)。
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  • 影响因子:
    0
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  • 通讯作者:
Shin-ichi Izumi, et al: "Electron microscopic techniques for immuno-histochemistry and Southwestern histochemistry (Abstract in English)"Journal of Electron Microscopy Technology for Medicine and Biology. 15(1). 64-69 (2000)
Shin-ichi Izumi 等人:“免疫组织化学和西南组织化学的电子显微镜技术(英文摘要)”医学和生物学电子显微镜技术杂志。
  • DOI:
  • 发表时间:
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  • 影响因子:
    0
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  • 通讯作者:
Masashi Shin,et al.: "Expression of cAMP responsive element binding protein (CREB) in livers of CCl_4-injected mouse"Acta Anatomica Nipponica. 74(1). 141 (1999)
Masashi Shin 等人:“cAMP 反应元件结合蛋白 (CREB) 在注射 CCl_4 的小鼠肝脏中的表达”Acta Anatomica Nipponica。
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    0
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IZUMI Shin-ichi其他文献

IZUMI Shin-ichi的其他文献

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{{ truncateString('IZUMI Shin-ichi', 18)}}的其他基金

Interaction between neuromodulation and brain machine interface:development of innovative technique
神经调节与脑机接口的相互作用:创新技术的发展
  • 批准号:
    23650314
  • 财政年份:
    2011
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Transcranial Magnetic Stimulation combined with imitation training recruiting Mirror Neuron System
经颅磁刺激结合模仿训练招募镜像神经元系统
  • 批准号:
    20240056
  • 财政年份:
    2008
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Molecular cytochemical analysis of correlation,between nuclear distribution of transcription factors and transcriptional activation or cell differentiation inductivity
转录因子核分布与转录激活或细胞分化诱导性相关性的分子细胞化学分析
  • 批准号:
    18590189
  • 财政年份:
    2006
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
The effect of a communication skills training based on coaching method for physician specializing in stroke
基于辅导法的脑卒中专科医师沟通技巧培训的效果
  • 批准号:
    18300181
  • 财政年份:
    2006
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Molecular Histo-cytochemical Analysis of Gene Expression Regulation by Artificial Control of Transcription Factors
通过人工控制转录因子进行基因表达调控的分子组织细胞化学分析
  • 批准号:
    15590161
  • 财政年份:
    2003
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Recovery of motor function by noninvasive stimulation of the human brain.
通过对人脑的无创刺激来恢复运动功能。
  • 批准号:
    10838039
  • 财政年份:
    1998
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Molecular Histochemical Analysis on Nuclear Distribution of Transcription Regulatory Factors and the Transcriptional Activity of the Genes.
转录调控因子核分布和基因转录活性的分子组织化学分析。
  • 批准号:
    09670018
  • 财政年份:
    1997
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

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“PtdIns(4,5)P2(一种膜脂信使)如何在剪接斑点(细胞核内的无膜区室)中定位和调节?
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