Studies on the molecular evolution of MHC class I genes and their polymorphism

MHC I类基因的分子进化及其多态性研究

• 批准号: 11670330
• 负责人: HASHIMOTO Keiichiro
• 金额: $ 2.3万
• 依托单位: Fujita Health University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670330/
• 关键词: MHC; class I; polymorphism; molecular evolution; linkage; class II; conservation; T cell receptor; 脊椎動物; 遺伝子構造

The present study suggests that the essential features in the T cell recognition of the MHC class I molecule/peptide complex appear to have been established at the early stage of the vertebrate evolution. Our study revealed highly conserved nature of the MHC class I molecules, namely, conservation of the peptide-binding strategy and conservation of the interface between a T cell receptor and an MHC class I molecule. We also analysed the genomic structure of a cartilaginous fish classical MHC class I gene and found that the exon/intron organization of the classical MHC class I gene is, basically, highly conserved throughout the evolution of the jawed vertebrates. We further examined the linkage between the classical MHC class I and class II genes in the cartilaginous fish. The results indicated that the two MHC classes belong to the same linkage group in the genome of the cartilaginous fish in contrast to the teleost fish but in accordance with the other vertebrate groups such as mammals, birds, and amphibians. Therefore, the linkage between the genes of the two MHC classes appears to be primitive and to have a long evolutionary history.

本研究表明，MHC-I类分子/肽复合体的T细胞识别的基本特征似乎在脊椎动物进化的早期阶段就已经确立。我们的研究揭示了MHC I类分子的高度保守性，即多肽结合策略的保守性和T细胞受体与MHC I类分子之间的界面保守性。我们还分析了一个软骨鱼类经典MHC I类基因的基因组结构，发现经典MHC I类基因的外显子/内含子组织在有颌骨脊椎动物的进化过程中基本上是高度保守的。我们进一步研究了软骨鱼中经典的MHC I类和II类基因之间的联系。结果表明，这两个MHC类在软骨鱼基因组中属于相同的连锁群，与硬骨鱼相反，但与哺乳动物、鸟类和两栖动物等其他脊椎动物类群一致。因此，这两个MHC类的基因之间的连锁似乎是原始的，并且有很长的进化历史。

项目成果

Yuko Ohta et al.: "Primitive synteng of vertebrate major histocompatibility complex class I and class II genes"Proc.Natl.Acad.Sci.USA. 97・9. 4712-4717 (2000)

Yuko Ohta 等：“脊椎动物主要组织相容性复合体 I 类和 II 类基因的原始合成”Proc.Natl.Acad.Sci.USA 97·9 (2000)。

Ohta, Y., Okamura, K., McKinney, E.C., Bartl, S., Hashimoto, K., and Flajnik, M.F.: "Primitive synteny of vertebrate major histocompatibility complex class I and class II genes."Proc.Natl.Acad.Sci.USA. 97. 4712-4717 (2000)

Ohta, Y.、Okamura, K.、McKinney, E.C.、Bartl, S.、Hashimoto, K. 和 Flajnik, M.F.：“脊椎动物主要组织相容性复合物 I 类和 II 类基因的原始同线性。”Proc.Natl.Acad

Hashimoto,K.: "Conservation and diversification of MHC class I and its related molecules in vertebrates"Immunol.Rer.. 167. 81-100 (1999)

Hashimoto,K.：“脊椎动物中 MHC I 类及其相关分子的保护和多样化”Immunol.Rer.. 167. 81-100 (1999)

Otakake,M.: "The function of the most primitive MHC class I"Der.Comp.Immunol.. 24. S79-80 (2000)

Otakake,M.：“最原始 MHC I 类的功能”Der.Comp.Immunol.. 24. S79-80 (2000)

Hashimoto, K., Okamura, K., Yamaguchi, H., Ototake, M., Nakanishi, T., and Kurosawa, Y.: "Conservation and diversification of MHC class I and its related molecules in vertebrates."Immunol.Rev.. 167. 81-100 (1999)

Hashimoto, K.、Okamura, K.、Yamaguchi, H.、Ototake, M.、Nakanishi, T. 和 Kurosawa, Y.：“脊椎动物 I 类 MHC 及其相关分子的保护和多样化。”Immunol.Rev