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Studies on the molecular evolution of MHC class I genes and their polymorplusin

MHC I类基因及其多毛虫蛋白的分子进化研究

基本信息

批准号：
09670346
负责人：
HASHIMOTO Keiichiro
金额：
$ 1.92万
依托单位：
Fujita Health University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670346/
关键词：
MHC class I polymorphism molecular evolution クラスI 魚類

项目摘要

We report the isolation and extensive analyses of highly polymorphic MHC class I genes from sharks (Triakis scyllia), that belong to the most primitive vertebrate group with jaws, the cartilaginous fish. Predicted complete peptide-binding domains showed retention of the critical amino acid residues that would interact with antigenic peptide termini and revealed extensive allelic polymorphism that can be comparable to those of human classical MHC class I molecules. Mosaic structures are apparent in these domains suggesting recombinational mechanisms to create allelic diversity. The present study demonstrated establishment of the basic strategy for antigen-presentation employed by MHC class I molecules and complete divergence of two polymorphic MHC classes at a phylogenetically primitive stage of vertebrate evolution.The elucidation of the complete peptide-binding domains of the highly polymorphic shark MHC class I genes offered us an opportunity to examine the characteristics of their predicted protein products in the light of the latest advance in the structural studies on the MHC class I molecules. The results suggest that the fundamental characteristics in the T cell recognition of the MHC class I molecule/peptide complex are expected to have been established at the early stage of the vertebrate evolution. The elucidation of the typical classical class I molecules from fishes and also of some MHC class I-related molecules may help us to explore the common denominator of the ancient class I molecules.

我们报告了鲨鱼（Triakis scyllia）中高度多态性MHC I类基因的分离和广泛分析，鲨鱼属于最原始的有颌骨的脊椎动物群，即软骨鱼。预测的完整的肽结合结构域显示保留的关键氨基酸残基，将相互作用的抗原肽末端，并揭示了广泛的等位基因多态性，可以与人类经典的MHC I类分子。镶嵌结构是明显的，在这些领域建议重组机制，创造等位基因多样性。本研究证明了MHC I类分子抗原呈递的基本策略的建立，以及两种多态性MHC在脊椎动物进化的遗传学原始阶段的完全分化。高度多态性的鲨鱼MHC I类基因的结合结构域为我们提供了一个机会，根据最新的研究结果，研究它们预测的蛋白质产物的特征。MHC I类分子结构研究的进展。结果表明，在T细胞识别的MHC I类分子/肽复合物的基本特征，预计已建立在脊椎动物进化的早期阶段。对鱼类中典型的经典I类分子和一些MHC I类相关分子的阐明，有助于我们探索古老I类分子的共同点。