Elucidation of the molecular evolution of MHC family and the establishment of the acquired immune system

阐明MHC家族的分子进化和获得性免疫系统的建立

• 批准号: 15590443
• 负责人: HASHIMOTO Keiichiro
• 金额: $ 1.98万
• 依托单位: Fujita Health University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15590443/
• 关键词: MHC; molecular evolution; acquired immune system; vertebrates; antigen presentation; primitive; molecular family; natural immunity; 免疫システム; ドメイン

The MHC family with the characteristic structure has various important biological functions including the peptide-presentation to T cells by the classical MHC molecules. However, the molecular evolution of the MHC family has not been clarified yet. To further understand the origin and the diversity of the MHC family and the establishment of the acquired immune system, we have been investigating the MHC-like molecules from primitive animals such as the cartilaginous fish. The MHC family can be classified into two classes, the class I and the class II, based on amino acid sequences and structural characteristics. Previously, we demonstrated that the MHC family molecules exist in the most primitive jawed vertebrates, the cartilaginous fish, and that the two classes has already been established at the level of fish. Further, we reported the generality of the mechanisms that create exceptionally high polymorphism of the classical MHC molecules, which is the basis of the allograft rejection. In relation to the establishment of the two classes, we recently clarified a novel gene that possesses the characteristics of both classes, and revealed that it belongs to a very unique linkage group. In this study, we also analyzed the MHC-related genes from the standpoint of the evolution of the MHC region in the genome.

MHC家族具有独特的结构，具有多种重要的生物学功能，包括经典的MHC分子向T细胞提呈肽。然而，MHC家族的分子进化尚未阐明。为了进一步了解MHC家族的起源和多样性以及获得性免疫系统的建立，我们一直在研究软骨鱼等原始动物的MHC样分子。根据氨基酸序列和结构特征，MHC家族可分为两类，I类和II类。在此之前，我们证明了MHC家族分子存在于最原始的有颌脊椎动物软骨鱼中，并且这两类分子已经在鱼类水平上建立。此外，我们还报道了造成经典MHC分子异常高多态性的机制的一般性，这是同种异体移植排斥反应的基础。关于这两个类别的建立，我们最近澄清了一个新的基因，拥有这两个类别的特征，并揭示了它属于一个非常独特的连锁群。在这项研究中，我们还分析了MHC相关基因的立场，在基因组中的MHC区域的进化。

项目成果

Identification of an interferon gamma homologue in Fugu, Takifugu rubripes.

• DOI: 10.1016/j.fsi.2004.04.015
• 发表时间: 2004-10
• 期刊: Fish & shellfish immunology
• 影响因子: 4.7
• 作者: J. Zou;Y. Yoshiura;J. M. Dijkstra;M. Sakai;M. Ototake;C. Secombes

Hashimoto, K.: "The origin of the MHC family molecules."Recent Res.Devel.Immunity. (In press).

Hashimoto, K.：“MHC 家族分子的起源。”最近的 Res.Devel.Immunity。

Molecular cloning of C4 gene and identification of the class III complement region in the shark MHC

鲨鱼MHC C4基因的分子克隆及III类补体区的鉴定

• 发表时间: 2003
• 期刊: J. Immunol. 171
• 作者: Terado;et al.
• 通讯作者: et al.

Terado, T et al.: "Molecular cloning of C4 gene and identification of the class III complement region in the shark MHC"J.Immunol.. 171. 2461-2466 (2003)

Terado, T 等人：“C4 基因的分子克隆和鲨鱼 MHC 中 III 类补体区的鉴定”J.Immunol.. 171. 2461-2466 (2003)

Interchromosomal duplication of MHC class I region in rainbow trout (Oncorhynchus mykiss), a species with presumable recent tetraploidy.

虹鳟鱼 (Oncorhynchus mykiss) 中 MHC I 类区域的染色体间重复，该物种可能具有近期四倍体。

• 发表时间: 2005
• 期刊: Immunogenetics (発表予定)
• 作者: Shiina;T.;Dijkstra;J.M.et al.
• 通讯作者: J.M.et al.