Analysis of susceptible genes of fatty liver

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We have developed NSY mice which are models of type 2 diabetes mellitus. These mice are also developed fatty liver in time-dependent manner. In this research, we have isolated the genes which showed the differential expression in the fatty liver of NSY mice in comparison with the genes expressed in the liver in C3H/He mice using PCR-based cDNA subtraction method which we had already developed. The earlier method analyzed the BamHI fragment to avoid the undesirable bias of PCR-amplification by difference of DNA length. However this method may cause pseudo-negative cDNA because only BamHI fragments had been analyzed. Thus, in this study, we analyzed not only BamHI fragments, but also HindIII fragments of cDNA of the liver.ResultsBamHI fragmentNSY liver dominant-genes : 3 clones, glucuronosyltransferase, human EST, 1 unknown geneC3H dominant-genes : 4 clones, TFII-I, DRPLA, collagen typeIV, 1 unknown geneHindIII fragmentNSY liver dominant-genes : 3 unknown clonesC3H dominant-genes : 5 clones, Ser/Thr phosphatase, HMG CoA reductase, translation initiation factor, IAP, 1 unknown geneAlong with this subtraction analysis, the study on the position of the susceptible gene of fatty liver by linkage analysis using the crossing of NSY mice with C3H/He mice are now on going. The results of the subtraction analysis will lead a susceptible gene for fatty liver in combination with the linkage analysis.