Gut mucosal immunity in surgical stress.

手术应激中的肠道粘膜免疫。

• 批准号: 11671151
• 负责人: SHINOZAKI Masaru
• 金额: $ 2.56万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671151/
• 关键词: surgical stress; Inflammatory bowel disease; Gut mucosal immunity; neutrophil; lymphocyte; cytokines; 全身防御反応

We investigated the influence of enterocytes stimulated with inflammatory cytokines on polymorphonuclear neutrophil (PMN) and lymphocyte chemotaxis.Obtained results are as follows :A human enterocyte cell line (FHs74 Int.) was grown in the lower wells of a two-compartment system with standard medium solution (DMEM) containing 4000 mM Gln (n=12). After confluent monolayer formation, the enterocytes were stimulated for 24 hours with various doses of TNF-alpha (0-100 ng/mL). Then, we performed PMN and lymphocyte chemotaxis assay. Confluent human umbilical vein endothelial cell monolayers grown on the inserts were overlaid with PMN or lymphocyte (5x106/mL) isolated from healthy volunteers. The number of migrated PMN or lymphocyte into the lower wells was counted after 45 minutes by flow cytometry.Enterocytes stimulated with TNF-alpha upregulated PMN migration in α TNF-alpha-dose dependent manner.

我们研究了炎症因子刺激肠细胞对多形核中性粒细胞（PMN）和淋巴细胞趋化性的影响。获得的结果如下：人肠细胞系（FHs74 Int.）在含有4000 mM Gln （n=12）的标准培养基溶液（DMEM）的双室系统的下孔中生长。融合单层形成后，用不同剂量的tnf - α (0-100 ng/mL)刺激肠细胞24小时。然后，我们进行了PMN和淋巴细胞趋化试验。植入物上生长的合流人脐静脉内皮细胞单层覆盖从健康志愿者中分离的PMN或淋巴细胞（5 × 106/mL）。45分钟后用流式细胞术计数迁移到下孔的PMN或淋巴细胞的数量。经tnf - α刺激的肠细胞以α - tnf - α剂量依赖性方式上调PMN迁移。

项目成果

Ikeda S., Saito H., Hidemura A., Fukatsu K.: "Dietrestriction reduces chemokine production and exudation of polymorphonuclear neutrophils at local inflammatory sites"Clinical Nutrition. 19(Supplement1). 15 (2000)

Ikeda S.、Saito H.、Hidemura A.、Fukatsu K.：“饮食限制减少局部炎症部位趋化因子的产生和多形核中性粒细胞的渗出”临床营养。

Fukushima R., Saito H., Inoue T., Fukatsu K., Inaba T., Han I., Furukawa S., Lin M.T., Muto T.: "Prophylactic treatment with growth hormone and insulin-like growth factor I improve systemic bacerial clearance and survival in a murine model of burn-induced

Fukushima R.、Saito H.、Inoue T.、Fukatsu K.、Inaba T.、Han I.、Furukawa S.、Lin M.T.、Muto T.：“使用生长激素和胰岛素样生长因子 I 进行预防性治疗可改善全身性

Furukawa S., Saito H., Lin M.T., Futakawa N., Yasuhara H., Kimura W., Inagawa H., Ono K., Maekawa K., Muto T.: "Enteral administration of glutamine in purulent peritonitis"Nutrition. 15(1). 29-31 (1999)

Furukawa S.、Saito H.、Lin M.T.、Futakawa N.、Yasuhara H.、Kimura W.、Inakawa H.、Ono K.、Maekawa K.、Muto T.：“化脓性腹膜炎中谷氨酰胺的肠内给药”营养。

Inoue T., Saito H., Ikeda S., Furukawa S., Han I., Matsuda T., Hidemura A.: "Predicting infectious complications in patients undergoing gastrointestinal surgery using preoperative untritional and metabolic variables"Clinical Nutrition. 18(Sup1). 25 (1999)

Inoue T.、Saito H.、Ikeda S.、Furukawa S.、Han I.、Matsuda T.、Hidemura A.：“使用术前非营养和代谢变量预测接受胃肠手术的患者的感染并发症”临床营养。

Ikeda S,Saito H,Inoue T, et al.: "Malnutrition impairs CD11b/CD18 expression on circulating polymorphonuclear neutrophils and subsequent exudation into inflammatory sites in the earlyphase of glycogen-induced murine peritonitis. "JPEN. 24(5). 276-279 (200

Ikeda S、Saito H、Inoue T 等人：“营养不良会损害循环多形核中性粒细胞的 CD11b/CD18 表达，并随后在糖原诱导的小鼠腹膜炎的早期渗出到炎症部位。”JPEN。