Gut mucosal immunity in surgical stress.

本实验研究了炎性细胞因子刺激的肠上皮细胞对中性粒细胞（PMN）和淋巴细胞趋化性的影响。在具有含4000 mM Gln的标准培养基溶液（DMEM）的双室系统的下部威尔斯孔中生长（n=12）。融合单层形成后，用不同剂量的TNF-α（0-100 ng/mL）刺激肠细胞24小时。然后，我们进行了中性粒细胞和淋巴细胞趋化性测定。将在插入物上生长的汇合的人脐静脉内皮细胞单层用从健康志愿者分离的PMN或淋巴细胞（5 × 106/mL）覆盖。45 min后用流式细胞仪计数PMN或淋巴细胞迁移到下威尔斯孔的数量，TNF-α刺激的肠细胞以α TNF-α剂量依赖的方式上调PMN的迁移。

We investigated the influence of enterocytes stimulated with inflammatory cytokines on polymorphonuclear neutrophil (PMN) and lymphocyte chemotaxis.Obtained results are as follows :A human enterocyte cell line (FHs74 Int.) was grown in the lower wells of a two-compartment system with standard medium solution (DMEM) containing 4000 mM Gln (n=12). After confluent monolayer formation, the enterocytes were stimulated for 24 hours with various doses of TNF-alpha (0-100 ng/mL). Then, we performed PMN and lymphocyte chemotaxis assay. Confluent human umbilical vein endothelial cell monolayers grown on the inserts were overlaid with PMN or lymphocyte (5x106/mL) isolated from healthy volunteers. The number of migrated PMN or lymphocyte into the lower wells was counted after 45 minutes by flow cytometry.Enterocytes stimulated with TNF-alpha upregulated PMN migration in α TNF-alpha-dose dependent manner.