HUMAN BREAST CANCER CELLS UNDER SERUM-FREE CULTURE. ITS HORMONE-DEPENDENCY AND APPLICATION TO THE PRIMARY CULTURE.

无血清培养下的人类乳腺癌细胞。

• 批准号: 11671172
• 负责人: KIMIJIMA Izo
• 金额: $ 1.73万
• 依托单位: Fukushima Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671172/
• 关键词: Human breast cancer cells; Serum-free culture; Hormone-dependency; Collagen-gel matrix; Primary culture; Fibroblast cell; 新血管誘導; コラーゲンゲルマトリック; 乳癌; 細胞間相互作用; 繊維芽細胞; HUVEC; 混合培養; 血管新生; コラーゲンゲル

Assessment of hormone-dependency of breast cancer cells in humen to estoradiol (E2) and progesterone (Pg) has been made by making serum-free culture of the cells, and primary culture of breast cancer cells is made by this method. The results are as follows: E2 promotes proliferation of estrogen receptor (ER) positive cells in low concentrations but rather inhibits it in high concentrations. This inhibitory effect is also observed in ER negative cells. If the mode of this inhibitory effect is compared with the cellular dynamics of Tamoxifen (TAM) which binds predominantly to ER, the cellular dynamics of E2 in high concentrations resembles that of TAM, from which some influence being exerted on the binding site of TAM by high concentrations of E2 is presumed. At binding of E2 to ER, S phase fraction increases significantly for 24 hours of culture if analyzed from the cellular dynamics, proving that the binding occurs comparatively in a short period. Inhibition of cellular proliferation by PgR depends on the concentration. PgR is not induced with only E2, and interference of other factors is suggested in the induction. Proliferation of the initially cultured cells is promoted by E2 although ER is present or not.

通过对人乳腺癌细胞进行无血清培养，评价了人乳腺癌细胞对雌二醇（E2）和孕酮（Pg）的依赖性，并通过该方法进行了乳腺癌细胞的原代培养。结果如下：E2在低浓度下促进雌激素受体（ER）阳性细胞的增殖，但在高浓度下抑制它。在ER阴性细胞中也观察到这种抑制作用。如果将这种抑制作用的模式与主要与ER结合的他莫昔芬（TAM）的细胞动力学进行比较，则高浓度E2的细胞动力学类似于TAM的细胞动力学，由此推测高浓度E2对TAM的结合位点施加了一些影响。在E2与ER结合时，如果从细胞动力学分析，S期分数在培养24小时内显著增加，证明结合发生在相对较短的时间内。PgR对细胞增殖的抑制取决于浓度。PgR仅用E_2不能诱导，提示诱导过程中有其它因素的干扰。尽管ER存在或不存在，但E2促进初始培养的细胞的增殖。

项目成果

Tsuchiya A, et al.: "Prognostic relevance of Tn expression in breast cancer"Breast Cancer. 6(3). 175-180 (1999)

Tsuchiya A 等人：“乳腺癌中 Tn 表达的预后相关性”乳腺癌。

Kimijima I, et al.: "Scattered fat invasion : An indication for poor prognosis in premenopausal, and for positive estrogen receptor in postmenopausal breast cancer patients"Oncology. 59. 25-30 (2000)

Kimijima I 等人：“分散的脂肪侵入：绝经前不良预后的指征，以及绝经后乳腺癌患者雌激素受体阳性的指征”肿瘤学。

Zhang GJ, et al.: "Apoptotic index correlates to bcl-2 and p53 protein expression, histological grade and prognosis in invasive breast cancers"Anticancer Research. 18. 1989-1998 (1998)

张国军等人：“细胞凋亡指数与浸润性乳腺癌中的 bcl-2 和 p53 蛋白表达、组织学分级和预后相关”抗癌研究。

Tsuchiya A, et al.: "Predicting axillary lymph node metastasis in breast cancer patients with tumors under 2 cm in size"Breast Cancer. 6(2). 167-170 (1999)

Tsuchiya A 等人：“预测肿瘤尺寸小于 2 厘米的乳腺癌患者的腋窝淋巴结转移”乳腺癌。

Zhang GJ, et al.: "Tamoxifen-induced apoptosis in breast cancer cells relates to down-regulation of bcl-2, but not bax and bcl-XL without alteration of p53 protein level1"Clinical Cancer Research. 5. 2971-2977 (1999)

张 GJ 等人：“他莫昔芬诱导的乳腺癌细胞凋亡与 bcl-2 的下调有关，但与 bax 和 bcl-XL 的下调无关，且不改变 p53 蛋白水平1”临床癌症研究。