Effect of immunosuppressant to neuropathic pain

免疫抑制剂对神经病理性疼痛的影响

• 批准号: 11671511
• 负责人: NAKAMURA Tadashi
• 金额: $ 2.3万
• 依托单位: Miyazaki Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671511/
• 关键词: Neuropathic pain; cyclosporine; betamethasone; rat; CCI; 神経結紮モデル; 機械的アロディニア; 免疫抑制薬; 局所麻酔

Using the chronic constriction model of Bennett and Xie (Pain 33, 1988), we investigated whether cyclosporine attenuates mechanical allodynia and hypersensitivity of rats for 70 days after surgery. In addition, we compared cyclosporine to betamethasone, which has potent anti-inflammatory effect, in treating neuropathic pain state.Thirty rats of 62, which showed tactile allodynia measured with von Frey hair at 2nd day after surgery, was randomly allocated to 6 groups (n=5 in each groups). Four groups received cyclosporine (0.01, 0.1, 1 and 10 mg/kg) intraperitoneally. The fifth group received betamethasone sodium phosphate 1 mg/kg, and the sixth group received equal volume of saline. Those injections were started on 2 days after sciatic nerve constriction and given daily for 20 days. Tactile allodynia, mechanical hyperalgesia and heat hyperalgesia were assessed for 70 days.Tactile allodynia was observed on both ipsi- and contralateral side of nerve constriction in all groups. Contralateral heat hyperalgesia was observed in saline and 0.01 mg/kg of cyclosporine injected groups. Similar contralateral mechanical hypersensitivity was not seen. Cyclosporine alleviated tactile allodynia, heat hypersensitivity and mechanical hypersensitivity in dose response manner, although betamethasone showed less potent effect. Body weight gain was less in betamethasone received rats than other groups on the last treatment day.These results indicate possible effects of cyclosporine to treat neuropathic pain. The treatment with cyclosporine seems to be effective even if it was started after nerve injury.

使用班尼特和Xie（Pain 33，1988）的慢性收缩模型，我们研究了环孢素是否在手术后70天减轻大鼠的机械性异常性疼痛和超敏反应。将术后第2天用von Frey毛测定触觉异常性疼痛的62只大鼠中的30只随机分为6组（每组5只）。4组分别腹腔注射环孢素0.01、0.1、1和10 mg/kg。第五组接受倍他米松磷酸钠1 mg/kg，第六组接受等体积的生理盐水。这些注射在坐骨神经收缩后2天开始，每天给药，持续20天。连续观察70天，在神经收缩的同侧和对侧均观察到触觉异常性痛，机械性痛敏和热性痛敏。在生理盐水和0.01 mg/kg环孢素注射组中观察到侧热痛觉过敏。未观察到类似的对侧机械超敏反应。环孢霉素可剂量反应性地减轻触觉异常性疼痛、热过敏和机械过敏，但贝那普利的作用较弱。在最后一个治疗日，接受环孢霉素的大鼠的体重增加低于其他组。这些结果表明环孢霉素可能对神经病理性疼痛有治疗作用。环孢素治疗似乎是有效的，即使它是在神经损伤后开始。