EFFECTS OF HYPOTHERMIA ON THE INDUCTION OF NO SYNTHASE EVOKED BY CYTOKINE IN VASCULAR SMOOTH MUSCLE

低温对血管平滑肌细胞因子诱导无合酶的影响

• 批准号: 11671519
• 负责人: MAEDA Hiroshi
• 金额: $ 1.28万
• 依托单位: WAKAYAMA MEDICAL COLLEGE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671519/
• 关键词: VASCULAR SMOOTH MUSCLE; CYTOKINE; NO SYNTHASE; HYPOTHERMIA

The purpose of this study was to clarify the effect of hypothermia on IL-1β evoked iNOS induction in vascular smooth muscle.Exposure of rat aortic rings without endothelium to IL-1β for 5h in 37℃ Krebs Ringer solution significantly attenuated the contractile responses to phenylephrine and KCl. These attenuations were reduced when exposed to IL-1β for 5h in 27℃ Krebs Rnger solution. Exposure of the endothelium-denuded rat aorta to IL-1β for 5 h in 37℃ Krebs Ringer solution caused an increase in the tissue cGMP contents. The levels of cGMP measured in aortic strips that were exposed to IL-1β for 5h in 27℃ Krebs Ringer solution were significantly lower than those in 37℃. The expressions of iNOS mRNA and iNOS protein evoked by the exposure to IL-1β for 5h in 37℃ Krebs Ringer solution were attenuated by the exposure to IL-1β in 27℃.The present study demonstrated that hypothermia attenuated the IL-1β evoked iNOS induction in vascular smooth muscle. These results suggest that iNOS induction may participate in the mechanism of ischemic brain damage and that the attenuation of iNOS induction may contribute the brain protection with hypothermia.

本研究的目的是阐明低温对血管平滑肌中IL-1β诱导的iNOS诱导的影响。将无内皮的大鼠主动脉环在37℃ Krebs Ringer溶液中暴露于IL-1β 5h显着减弱对去氧肾上腺素和KCl的收缩反应。当在 27℃ Krebs Rnger 溶液中暴露于 IL-1β 5 小时时，这些衰减降低。将去内皮大鼠主动脉暴露于37℃ Krebs Ringer 溶液中的IL-1β 5 h 引起组织cGMP 含量增加。在27℃克雷布斯林格溶液中暴露于IL-1β5h的主动脉条中测得的cGMP水平显着低于37℃中的水平。在37℃ Krebs Ringer溶液中暴露于IL-1β 5小时诱发的iNOS mRNA和iNOS蛋白表达在27℃暴露于IL-1β中减弱。本研究表明低温减弱了血管平滑肌中IL-1β诱发的iNOS诱导。这些结果表明iNOS诱导可能参与缺血性脑损伤的机制，并且iNOS诱导减弱可能有助于低温保护脑。

项目成果

H.Maeda, H.Iranami, M.Yamamoto, K.Ogawa, Y.Morikawa, E.Senba, Y.Hatano: "Halothane but not isoflurane attenuates IL-1b induced nitric oxide synthase in vascular smooth muscle."Anesthesiology. (in press). (2001)

H.Maeda、H.Iranami、M.Yamamoto、K.Okawa、Y.Morikawa、E.Senba、Y.Hatano：“氟烷而非异氟烷会减弱血管平滑肌中 IL-1b 诱导的一氧化氮合酶。”麻醉学。

H.Kinoshita, T.Ishikawa, Y.Hatano: "Differential effects of lidocaine and mexiletine on relaxations to ATP-sensitive potassium channel openers in rat aortas"Anesthesiology. 90. 1165-1170 (1999)

H.Kinoshita、T.Ishikawa、Y.Hatano：“利多卡因和美西律对大鼠主动脉 ATP 敏感钾通道开放剂松弛的不同影响”麻醉学。

H.Kinoshita, T.Kakutani, H.Iranami, Y.Hatano: "The role of oxygen-derived free radicals in augmented relaxations to levcromakalim in the aorta from hypertensive rats."Jpn J Pharmacol. 85. 29-33 (2001)

H.Kinoshita、T.Kakutani、H.Iranami、Y.Hatano：“氧自由基在增强高血压大鼠主动脉 levcromakalim 松弛中的作用。”Jpn J Pharmacol。

畑埜義雄,木下浩之: "摘出脳血管の反応特異性"臨床麻酔. 24. 311-325 (2000)

Yoshio Hatano、Hiroyuki Kinoshita：“离体脑血管的反应特异性”临床麻醉 24. 311-325 (2000)。

H.Kinoshita, S.Iwahashi, T.Kakutani, K.Mizumoto, H.Iranami, Y.Hatano: "The role of endothelium-derived nitric oxide in relaxations to levcromakalim in the rat aorta."Jpn J Pharmacol. 81. 362-366 (1999)

H.Kinoshita、S.Iwahashi、T.Kakutani、K.Mizumoto、H.Iranami、Y.Hatano：“内皮源性一氧化氮在大鼠主动脉 levcromakalim 松弛中的作用。”Jpn J Pharmacol。