Role for corneal fibroblasts in the pathogenesis of allergic conjunctival diseases

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1. Synergistic induction of eotaxin expression by corneal fibroblast by TNF-α and IL-4 or IL-13.The effect of TNF-α, IL-4 or IL-13 on the expression of chemokine eotaxin by cultured human corneal fibroblasts were examined by enzyme-linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR). Without the stimulation by cytokines, the expression of eotaxin protein by these cells was below detectable level. These cells expressed eotaxin protein by the stimulation of TNF-α, IL-4 or IL-13 in a dose-dependent manner. The expression of eotaxin protein and mRNA was synergistically increased by the stimulation of TNF-α and IL-4 or IL-13.2. Synergistic induction of thymus and activation-regulated chemokine (TARC) by TNF-α and IL-4The effect of TNF-α and IL-4 on the expression of chemokine TARC by cultured human corneal fibroblasts were examined by ELISA and quantitative RT-PCR.Corneal fibroblast did not express detectable TARC protein or mRNA spontaneously or by the stimulation of TNF-α or IL-4. When these cells were stimulated in the presence of both TNF-α and IL-4, these cells expressed TARC protein and mRNA.