Role for corneal fibroblasts in the pathogenesis of allergic conjunctival diseases

角膜成纤维细胞在过敏性结膜疾病发病机制中的作用

• 批准号: 11671741
• 负责人: KUMAGAI Naoki
• 金额: $ 2.3万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671741/
• 关键词: Corneal fibroblasts; eotaxin; thymus and activation-regulated chemokines; TNG-α; IL-4; IL-13; アレルギー性結膜炎; 角膜上皮細胞

1. Synergistic induction of eotaxin expression by corneal fibroblast by TNF-α and IL-4 or IL-13.The effect of TNF-α, IL-4 or IL-13 on the expression of chemokine eotaxin by cultured human corneal fibroblasts were examined by enzyme-linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR). Without the stimulation by cytokines, the expression of eotaxin protein by these cells was below detectable level. These cells expressed eotaxin protein by the stimulation of TNF-α, IL-4 or IL-13 in a dose-dependent manner. The expression of eotaxin protein and mRNA was synergistically increased by the stimulation of TNF-α and IL-4 or IL-13.2. Synergistic induction of thymus and activation-regulated chemokine (TARC) by TNF-α and IL-4The effect of TNF-α and IL-4 on the expression of chemokine TARC by cultured human corneal fibroblasts were examined by ELISA and quantitative RT-PCR.Corneal fibroblast did not express detectable TARC protein or mRNA spontaneously or by the stimulation of TNF-α or IL-4. When these cells were stimulated in the presence of both TNF-α and IL-4, these cells expressed TARC protein and mRNA.

1.肿瘤坏死因子-α与IL-4或IL-13协同诱导角膜成纤维细胞表达嗜酸性粒细胞趋化因子。采用酶联免疫吸附试验和逆转录聚合酶链式反应检测肿瘤坏死因子-α、IL-4或IL-13对培养的人角膜成纤维细胞表达趋化因子嗜酸性粒细胞趋化因子的影响。在没有细胞因子刺激的情况下，这些细胞的嗜酸性粒细胞趋化蛋白的表达低于可检测的水平。这些细胞在肿瘤坏死因子-α、IL-4或IL-13的刺激下以剂量依赖的方式表达嗜酸性粒细胞趋化蛋白。肿瘤坏死因子-α与IL-4或IL-13协同刺激Eoaxin蛋白和基因的表达。IL-4和肿瘤坏死因子-α对体外培养的人角膜成纤维细胞表达趋化因子TARC的影响采用ELISA法和定量RT-PCR法检测α和IL-4对培养的人角膜成纤维细胞表面趋化因子TARC表达的影响。角膜成纤维细胞不能自发表达TARC蛋白，也不能在α或IL-4刺激下表达。当肿瘤坏死因子-α和IL-4同时作用于这些细胞时，这些细胞表达TARC蛋白和基因。

项目成果

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郝金林等：“加拉丁对铜绿假单胞菌降解胶原蛋白的影响”实验眼科研究。

Hao,J-L: "Galardin inhibitis collagen degradation by rabbit keratocytes by inhibiting the activation of pro-matrix metallo proteinases"Exp Eye Res. 68. 565-572 (1999)

郝，J-L：“加拉丁通过抑制前基质金属蛋白酶的激活来抑制兔角膜细胞的胶原蛋白降解”Exp Eye Res。

Hao,J-L: "Effect of galardign on collagen degradation by pseudomonas aeruginosa"Exp Eye Res. 69. 595-601 (1999)

郝J-L：“加拉良对铜绿假单胞菌降解胶原蛋白的影响”Exp Eye Res。

Komori A et al: "A case of vernal keratoconjunctivitis in advanced age"Japanese Journal of Clinical Ophthalmology. 54 (1). 25-29 (2000)

小森A等：“高龄春季角结膜炎一例”日本临床眼科杂志。

Kumagai,N: "Synergistic induction of eotaxin expression in human keratocytes by TNF-α and IL-4 or IL-13"Invest Ophthalmol Vis Sci. (in press).

Kumagai, N：“TNF-α 和 IL-4 或 IL-13 协同诱导人角膜细胞中嗜酸细胞趋化因子的表达”Invest Ophamol Vis Sci（正在出版）。