Role of corneal-conjunctival interaction on the pathogenesis of allergic conjunctivitis

角膜-结膜相互作用在过敏性结膜炎发病机制中的作用

• 批准号: 17591837
• 负责人: KUMAGAI Naoki
• 金额: $ 2.24万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591837/
• 关键词: allergic conjunctiva; cornea; chemokine; adhesion molecule; cytokine

Recent studies have shown the correlation between the degree of corneal lesion and that of conjunctival inflammation in individuals with allergic conjunctivitis. It is also demonstrated by several researchers, including us, that corneal fibroblasts are the promoter of conjunctival inflammation through their potent release/expression of many cytokines, chemokines and adhesion molecules. In contrast, corneal epithelial cells are considered to be the quencher of conjunctival inflammation by their ability to separate corneal cells from bioactive substances in the tear fluids.In the current study, using rat experimental allergic conjunctivitis models, we examined the effect of corneal epithelial peeling on 1) clinical picture of conjunctivitis, 2)release/expression of cytokines, chemokines and adhesion molecules. We also examined 3) the effect of conjunctival allergic inflammation on the repair of corneal epithelium.Result1) Corneal epithelial peeling enhanced the clinical picture of the late phase of allergic conjunctivitis. It also enhanced the number of eosinophils in the conjunctiva at the late phase. It had no effect on the early phase.2) Corneal epithelial peeling enhanced the release/expression of chemokines TARC, RANTES, MCP-I and adhesion molecule ICAM-1 in the conjunctiva. It enhanced the release/expression of chemokines TARC, RANTES, IP-10, MCP-1 and adhesion molecule ICAM-1 and VCAM-1 in the cornea.3) Induction of allergic conjunctivitis delayed the repair of corneal epithelium.DiscussionIn rat experimental allergic conjunctivitis model, corneal epithelium serves as a quencher of allergic inflammation. Corneal epithelial peeling enhances the late phase of allergic reaction possibly, at least in part, through the activation of structural cells of the cornea. Delayed epithelial repair of the cornea contribute the exacerbation of allergic inflammation in the conjunctiva.

最近的研究表明，过敏性结膜炎患者的角膜病变程度与结膜炎症程度之间存在相关性。包括我们在内的一些研究人员也证明，角膜成纤维细胞通过释放/表达许多细胞因子、趋化因子和黏附分子而成为结膜炎症的促进者。相比之下，角膜上皮细胞被认为是结膜炎症的熄灭剂，因为它们能够将角膜细胞与泪液中的活性物质分开。在本研究中，我们利用大鼠实验性过敏性结膜炎模型，研究了角膜上皮剥离对1)结膜炎临床表现的影响，2)细胞因子、趋化因子和黏附分子的释放/表达。3)结膜过敏性炎症对角膜上皮修复的影响。结果1)角膜上皮剥离增强了过敏性结膜炎晚期的临床表现。晚期结膜中嗜酸性粒细胞数量增加。2)角膜上皮剥离可促进结膜趋化因子TARC、RANTES、MCP-I和黏附分子ICAM-1的释放/表达。促进角膜中趋化因子TARC、RANTES、IP-10、MCP-1和黏附分子ICAM-1、VCAM-1的释放/表达。3)过敏性结膜炎的诱导延缓了角膜上皮的修复。角膜上皮剥离可能至少部分地通过激活角膜结构细胞来增强过敏反应的晚期。延迟的角膜上皮修复加剧了结膜的过敏性炎症。

项目成果

Analysis of the interaction between IFN-γ and IFN-γR in the effector phase of experimental murine allergic conjunctivitis.

实验性小鼠过敏性结膜炎效应期IFN-γ与IFN-γR相互作用分析

• 期刊: Immunology Letters (in press)
• 作者: Sagara N;Kawaji T;Takano A;Inomata Y;Inatani M;Fukushima M;Tanihara H.;Fukushima A et al.;Fukushima A et al.;Fukushima A et al.;Fukushima A et al.;Fukushima A et al.;Fukushima A et al.;Naoki Kumagai et al.;Fukushima A et al.;Kazutaka Yamamoto et al.;Fukushima A et al.;Ying Lu et al.;Ken Fukuda et al.;Fukushima A et al.;Ken Fukuda et al.;Ying Lu et al.;Fukushima A et al.;Atsuki Fukushima et al.;Fukushima A et al.;Atsuki Fukushima et al.;Fukushima A et al.;Naoki Kumagai et al.;Ozaki A et al.;Kazutaka Yamamoto et al.;Fukushima A et al.;Fukushima A et al.;Ying Lu et al.;福島 敦樹;Ken Fukuda et al.;Ken Fukuda et al.;Ying Lu et al.;Atsuki Fukushima et al.;Atsuki Fukushima et al.;Kazutaka Yamamoto et al.;Ying Lu et al.;Ken Fukuda et al.;Ken Fukuda et al.;Ying Lu et al.;Atsuki Fukushima et al.;Naoki Kumagai et al.;Ying Lu et al.;Ken Fukuda et al.;Ying Lu et al.;Ken Fukuda et al.;Ying Lu et al.;澤口昭一;Ken Fukuda et al.;澤口昭一;Atsuki Fukushima et al.
• 通讯作者: Atsuki Fukushima et al.

Inhibition of matrix metalloproteinase-3 synthesis in human conjunctival fibroblasts by interleukin-4 or interleukin-13

• DOI: 10.1167/iovs.05-1261
• 发表时间: 2006-07-01
• 期刊: INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
• 影响因子: 4.4
• 作者: Fukuda, K;Fujitsu, Y;Nishida, T
• 通讯作者: Nishida, T

Inhibition by triptolide of chemokine, proinfiammatory cytokine, and adhesion molecule expression induced by lipopolysaccharide in corneal fibroblasts.

雷公藤内酯醇抑制角膜成纤维细胞中脂多糖诱导的趋化因子、促炎性细胞因子和粘附分子的表达。

• 发表时间: 2006
• 期刊: Investigative Ophthalmology ＆ Visual Science 47-9
• 作者: Sagara N;Kawaji T;Takano A;Inomata Y;Inatani M;Fukushima M;Tanihara H.;Fukushima A et al.;Fukushima A et al.;Fukushima A et al.;Fukushima A et al.;Fukushima A et al.;Fukushima A et al.;Naoki Kumagai et al.;Fukushima A et al.;Kazutaka Yamamoto et al.;Fukushima A et al.;Ying Lu et al.;Ken Fukuda et al.;Fukushima A et al.;Ken Fukuda et al.;Ying Lu et al.;Fukushima A et al.;Atsuki Fukushima et al.;Fukushima A et al.;Atsuki Fukushima et al.;Fukushima A et al.;Naoki Kumagai et al.;Ozaki A et al.;Kazutaka Yamamoto et al.;Fukushima A et al.;Fukushima A et al.;Ying Lu et al.;福島 敦樹;Ken Fukuda et al.;Ken Fukuda et al.;Ying Lu et al.
• 通讯作者: Ying Lu et al.

Inhibition by triptolide of chemokine, proinflammatory cytokine, and adhesion molecule expression induced by lipopolysaccaride in corneal fibroblasts.

雷公藤甲素抑制角膜成纤维细胞中脂多糖诱导的趋化因子、促炎细胞因子和粘附分子的表达。

• 发表时间: 2006
• 期刊: Investigative Ophthalmology ＆ Visual Science 47・9
• 作者: Sagara N;Kawaji T;Takano A;Inomata Y;Inatani M;Fukushima M;Tanihara H.;Fukushima A et al.;Fukushima A et al.;Fukushima A et al.;Fukushima A et al.;Fukushima A et al.;Fukushima A et al.;Naoki Kumagai et al.;Fukushima A et al.;Kazutaka Yamamoto et al.;Fukushima A et al.;Ying Lu et al.;Ken Fukuda et al.;Fukushima A et al.;Ken Fukuda et al.;Ying Lu et al.;Fukushima A et al.;Atsuki Fukushima et al.;Fukushima A et al.;Atsuki Fukushima et al.;Fukushima A et al.;Naoki Kumagai et al.;Ozaki A et al.;Kazutaka Yamamoto et al.;Fukushima A et al.;Fukushima A et al.;Ying Lu et al.;福島 敦樹;Ken Fukuda et al.;Ken Fukuda et al.;Ying Lu et al.;Atsuki Fukushima et al.;Atsuki Fukushima et al.;Kazutaka Yamamoto et al.;Ying Lu et al.;Ken Fukuda et al.;Ken Fukuda et al.;Ying Lu et al.
• 通讯作者: Ying Lu et al.

Role of nuclear factor-κB in interleukin-1-induced collagen degradation by corneal fibroblasts

• DOI: 10.1016/j.exer.2006.02.008
• 发表时间: 2006-09-01
• 期刊: EXPERIMENTAL EYE RESEARCH
• 影响因子: 3.4
• 作者: Lu, Ying;Fukuda, Ken;Nishida, Teruo
• 通讯作者: Nishida, Teruo