Role of corneal-conjunctival interaction on the pathogenesis of allergic conjunctivitis
Role of corneal-conjunctival interaction on the pathogenesis of allergic conjunctivitis
角膜-结膜相互作用在过敏性结膜炎发病机制中的作用
批准号：
17591837
负责人：
KUMAGAI Naoki
金额：
$2.24万
依托单位：
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
项目状态：
已结题
起止时间：
2005 至 2006
中文摘要
翻译
最近的研究表明，过敏性结膜炎患者角膜病变程度与结膜炎症程度之间存在相关性。包括我们在内的一些研究人员也证明，角膜成纤维细胞通过其有效释放/表达许多细胞因子、趋化因子和粘附分子而成为结膜炎症的促进剂。相比之下，角膜上皮细胞被认为是结膜炎症的淬灭剂，因为它们具有将角膜细胞与泪液中的生物活性物质分离的能力。在当前的研究中，使用大鼠实验性过敏性结膜炎模型，我们检查了角膜上皮剥离对1）结膜炎的临床表现，2）细胞因子、趋化因子和粘附分子的释放/表达的影响。结果1）角膜上皮剥脱可增强变应性结膜炎晚期的临床表现，但对角膜上皮的损伤程度无明显影响。它还增加了结膜中的嗜酸性粒细胞的数量在后期阶段。2）角膜上皮剥脱促进结膜组织中趋化因子TARC、RANTES、MCP-1和粘附分子ICAM-1的释放和表达。结论：（1）在大鼠实验性变应性结膜炎模型中，角膜上皮细胞作为变应性炎症的抑制剂，具有抑制炎症反应的作用。（2）在大鼠实验性变应性结膜炎模型中，角膜上皮细胞具有抑制炎症反应的作用。角膜上皮剥脱可能至少部分地通过激活角膜的结构细胞而增强变态反应的晚期。角膜上皮修复的延迟导致结膜过敏性炎症的恶化。
英文摘要
Recent studies have shown the correlation between the degree of corneal lesion and that of conjunctival inflammation in individuals with allergic conjunctivitis. It is also demonstrated by several researchers, including us, that corneal fibroblasts are the promoter of conjunctival inflammation through their potent release/expression of many cytokines, chemokines and adhesion molecules. In contrast, corneal epithelial cells are considered to be the quencher of conjunctival inflammation by their ability to separate corneal cells from bioactive substances in the tear fluids.In the current study, using rat experimental allergic conjunctivitis models, we examined the effect of corneal epithelial peeling on 1) clinical picture of conjunctivitis, 2)release/expression of cytokines, chemokines and adhesion molecules. We also examined 3) the effect of conjunctival allergic inflammation on the repair of corneal epithelium.Result1) Corneal epithelial peeling enhanced the clinical picture of the late phase of allergic conjunctivitis. It also enhanced the number of eosinophils in the conjunctiva at the late phase. It had no effect on the early phase.2) Corneal epithelial peeling enhanced the release/expression of chemokines TARC, RANTES, MCP-I and adhesion molecule ICAM-1 in the conjunctiva. It enhanced the release/expression of chemokines TARC, RANTES, IP-10, MCP-1 and adhesion molecule ICAM-1 and VCAM-1 in the cornea.3) Induction of allergic conjunctivitis delayed the repair of corneal epithelium.DiscussionIn rat experimental allergic conjunctivitis model, corneal epithelium serves as a quencher of allergic inflammation. Corneal epithelial peeling enhances the late phase of allergic reaction possibly, at least in part, through the activation of structural cells of the cornea. Delayed epithelial repair of the cornea contribute the exacerbation of allergic inflammation in the conjunctiva.
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Analysis of the interaction between IFN-γ and IFN-γR in the effector phase of experimental murine allergic conjunctivitis.
实验性小鼠过敏性结膜炎效应期IFN-γ与IFN-γR相互作用分析
实验性小鼠过敏性结膜炎效应期IFN-γ与IFN-γR相互作用分析
DOI：
--
发表时间：
期刊：
Immunology Letters (in press)
影响因子：
--
作者：
[Sagara N, Kawaji T, Takano A, Inomata Y, Inatani M, Fukushima M, Tanihara H., Fukushima A et al., Fukushima A et al., Fukushima A et al., Fukushima A et al., Fukushima A et al., Fukushima A et al., Naoki Kumagai et al., Fukushima A et al., Kazutaka Yamamoto et al., Fukushima A et al., Ying Lu et al., Ken Fukuda et al., Fukushima A et al., Ken Fukuda et al., Ying Lu et al., Fukushima A et al., Atsuki Fukushima et al., Fukushima A et al., Atsuki Fukushima et al., Fukushima A et al., Naoki Kumagai et al., Ozaki A et al., Kazutaka Yamamoto et al., Fukushima A et al., Fukushima A et al., Ying Lu et al., 福島 敦樹, Ken Fukuda et al., Ken Fukuda et al., Ying Lu et al., Atsuki Fukushima et al., Atsuki Fukushima et al., Kazutaka Yamamoto et al., Ying Lu et al., Ken Fukuda et al., Ken Fukuda et al., Ying Lu et al., Atsuki Fukushima et al., Naoki Kumagai et al., Ying Lu et al., Ken Fukuda et al., Ying Lu et al., Ken Fukuda et al., Ying Lu et al., 澤口昭一, Ken Fukuda et al., 澤口昭一, Atsuki Fukushima et al.]
通讯作者：
Atsuki Fukushima et al.
DOI：
10.1167/iovs.05-1261
发表时间：
2006-07-01
期刊：
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
影响因子：
4.4
作者：
[Fukuda, K, Fujitsu, Y, Nishida, T]
通讯作者：
Nishida, T
Inhibition by triptolide of chemokine, proinfiammatory cytokine, and adhesion molecule expression induced by lipopolysaccharide in corneal fibroblasts.
雷公藤内酯醇抑制角膜成纤维细胞中脂多糖诱导的趋化因子、促炎性细胞因子和粘附分子的表达。
雷公藤内酯醇抑制角膜成纤维细胞中脂多糖诱导的趋化因子、促炎性细胞因子和粘附分子的表达。
DOI：
--
发表时间：
2006
期刊：
Investigative Ophthalmology ＆ Visual Science 47-9
影响因子：
--
作者：
[Sagara N, Kawaji T, Takano A, Inomata Y, Inatani M, Fukushima M, Tanihara H., Fukushima A et al., Fukushima A et al., Fukushima A et al., Fukushima A et al., Fukushima A et al., Fukushima A et al., Naoki Kumagai et al., Fukushima A et al., Kazutaka Yamamoto et al., Fukushima A et al., Ying Lu et al., Ken Fukuda et al., Fukushima A et al., Ken Fukuda et al., Ying Lu et al., Fukushima A et al., Atsuki Fukushima et al., Fukushima A et al., Atsuki Fukushima et al., Fukushima A et al., Naoki Kumagai et al., Ozaki A et al., Kazutaka Yamamoto et al., Fukushima A et al., Fukushima A et al., Ying Lu et al., 福島 敦樹, Ken Fukuda et al., Ken Fukuda et al., Ying Lu et al.]
通讯作者：
Ying Lu et al.
Inhibition by triptolide of chemokine, proinflammatory cytokine, and adhesion molecule expression induced by lipopolysaccaride in corneal fibroblasts.
雷公藤甲素抑制角膜成纤维细胞中脂多糖诱导的趋化因子、促炎细胞因子和粘附分子的表达。
雷公藤甲素抑制角膜成纤维细胞中脂多糖诱导的趋化因子、促炎细胞因子和粘附分子的表达。
DOI：
--
发表时间：
2006
期刊：
Investigative Ophthalmology ＆ Visual Science 47・9
影响因子：
--
作者：
[Sagara N, Kawaji T, Takano A, Inomata Y, Inatani M, Fukushima M, Tanihara H., Fukushima A et al., Fukushima A et al., Fukushima A et al., Fukushima A et al., Fukushima A et al., Fukushima A et al., Naoki Kumagai et al., Fukushima A et al., Kazutaka Yamamoto et al., Fukushima A et al., Ying Lu et al., Ken Fukuda et al., Fukushima A et al., Ken Fukuda et al., Ying Lu et al., Fukushima A et al., Atsuki Fukushima et al., Fukushima A et al., Atsuki Fukushima et al., Fukushima A et al., Naoki Kumagai et al., Ozaki A et al., Kazutaka Yamamoto et al., Fukushima A et al., Fukushima A et al., Ying Lu et al., 福島 敦樹, Ken Fukuda et al., Ken Fukuda et al., Ying Lu et al., Atsuki Fukushima et al., Atsuki Fukushima et al., Kazutaka Yamamoto et al., Ying Lu et al., Ken Fukuda et al., Ken Fukuda et al., Ying Lu et al.]
通讯作者：
Ying Lu et al.
DOI：
10.1016/j.exer.2006.02.008
发表时间：
2006-09-01
期刊：
EXPERIMENTAL EYE RESEARCH
影响因子：
3.4
作者：
[Lu, Ying, Fukuda, Ken, Nishida, Teruo]
通讯作者：
Nishida, Teruo
共 21 条
Research on the innate immune system of the cornea
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批准号：14571674
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项目类别：Grant-in-Aid for Scientific Research (C)
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资助金额：$2.24万
-
财政年份：2002
-
负责人：KUMAGAI Naoki
-
依托单位：
Role for corneal fibroblasts in the pathogenesis of allergic conjunctival diseases
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批准号：11671741
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项目类别：Grant-in-Aid for Scientific Research (C)
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资助金额：$2.3万
-
财政年份：1999
-
负责人：KUMAGAI Naoki
-
依托单位：
JOINT STUDY ON DEVELOPMENT OF NEW INERCALATION ELECTRODES
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批准号：11695036
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项目类别：Grant-in-Aid for Scientific Research (B)
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资助金额：$3.46万
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财政年份：1999
-
负责人：KUMAGAI Naoki
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依托单位：
国内基金
海外基金
TLR2影响角膜移植术后植片转归的机制研究
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批准号：81170887
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项目类别：面上项目
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资助金额：56.0万元
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批准年份：2011
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负责人：白浪
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依托单位：