Effect of Growth Factors on Cultured Human Hypertrophic Scar Fibroblasts

生长因子对培养的人增生性疤痕成纤维细胞的影响

• 批准号: 11671792
• 负责人: MORIGUCHI Takahiko
• 金额: $ 2.18万
• 依托单位: KAWASAKI MEDICAL SCHOOL
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671792/
• 关键词: Keloid; fibroblasts; growth factors; collagenase; 創傷治癒; 細胞増殖; Fibroblasts; TGF-β; TNF-α; コラーゲン代謝; 相互作用

Regarding wound repair, there are important but as yet unknown factors involved in the regulation of fibroblast function in the formation of hypertrophic scars and keloids. If these factors could be clarified, new therapies for keloids could be developed.Recently, some researchers have reported that growth factors and cytokines possess some abilities to control hypertrophic scar and keloid formation.The purpose of this study was to investigate whether the collagenase activity in human keloid fibroblasts is stimulated by two kinds of growth factors, TNF -α and TGF-β. Increased levels of collagenase activity were found in human keloid fibroblasts following exposure to TNF - α. A decreased level of collagenase activity was found in human keloid fibroblasts following exposure to both TNF - α and TGF- β. These results indicate that TGF- β can inhibit collagenase activity in human keloid fibroblasts stimulated by TNF - α, suggesting that growth factors and cytokines may play a therapeutic role in keloid formation.

关于伤口修复，在增生性瘢痕和瘢痕疙瘩的形成中，有重要但未知的因素参与成纤维细胞功能的调节。本研究旨在探讨肿瘤坏死因子-α（TNF -α）和转化生长因子-β（TGF-β）两种生长因子对瘢痕疙瘩成纤维细胞胶原酶活性的影响。在暴露于TNF - α后，在人瘢痕疙瘩成纤维细胞中发现胶原酶活性水平增加。在TNF - α和TGF- β暴露后，发现人瘢痕疙瘩成纤维细胞中胶原酶活性水平降低。这些结果表明，TGF- β可以抑制TNF - α刺激的人瘢痕疙瘩成纤维细胞的胶原酶活性，表明生长因子和细胞因子可能在瘢痕疙瘩形成中起治疗作用。

项目成果

山本雅之: "ヒト皮膚線維芽細胞に対する細胞増殖因子の影響について"ケロイド肥厚性瘢痕研究会記録集. 6. 27-33 (2001)

Masayuki Yamamoto：“关于细胞生长因子对人真皮成纤维细胞的影响”，增生性疤痕研究组记录，6. 27-33 (2001)。

MASAYUKI YAMAMOTO M.D.: "Effect of Growth Factors on Cultured Human Skin Fibroblasts"6th, Investigation of KELOID AND HYPERTROPHIC SCAR. 27-33 (2000)

MASAYUKI YAMAMOTO M.D.：“生长因子对培养的人类皮肤成纤维细胞的影响”6，疤痕疙瘩和肥厚性疤痕的调查。

山本雅之: "ヒト皮膚線維芽細胞に対する細胞増殖因子の影響について"第6回ケロイド肥厚性瘢痕研究会記録集. 6. 27-33 (2000)

Masayuki Yamamoto：“细胞生长因子对人真皮成纤维细胞的影响”第六届增生性疤痕研究组记录 6. 27-33 (2000)。

山本雅之: "ケロイド由来線維芽細胞に対するTNF-α,TGF-βの影響について"川崎医学会誌. 27-1. 7-9 (2001)

Masayuki Yamamoto：“TNF-α 和 TGF-β 对疤痕疙瘩来源的成纤维细胞的影响”川崎医学会杂志 27-1-9 (2001)。