Expression of genes and proteins of extracellular matrix molecules during differentiation of cementum and alveolar bone

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The present study was designed to investigate differentiation of cellular and extracellular matrix components in bone and cementum focusing on expressions of both gene and protein. Antibodies and RNA probes for type I collagen, osteonectin, osteopontin, bone sialoprotein and osteocalcin were prepared. Immunohistochemistry indicated that type I collagen constitutes the basic framework in the extracellular space and thereafter the noncollagenous proteins were deposited simultaneously by osteoblasts and involved in calcification cooperatively during rat mandiular osteogenesis (These results have been reported in Anat Embryol [2000] 202 : 31-37.). In situ hybridization demonstrated that gene expression of those extrcellular matrix molecules was closely related to differentiation of osteoblasts (These studies are in press for Histochem J [2001].) . Moreover, both immunohistochemistry and in situ hybridization showed spatial and temporal expression of the extracellular matrix molecules during formation of both cellular and acellular cementum in rat molar cementogenesis (These studies are in press for Histochem J [2001].). The present study suggested the distinctive expression pattern of genes and proteins of the extracellular matrix molecules during formation of cellular and acellular cementum and bone.