Protein dephosphorylation and apoptosis in osteoblasts
成骨细胞中的蛋白质去磷酸化和细胞凋亡
基本信息
- 批准号:11671806
- 负责人:
- 金额:$ 2.24万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1999
- 资助国家:日本
- 起止时间:1999 至 2000
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Several lines of evidence indicate that protein phosphorylation and dephosphorylation has been recognized as a key mechanism in cell proliferation, differentiation, and apoptosis in various tissues. Okadaic acid is a toxic polyether fatty acid produced by dinoflagellates and is a potent inhibitor of protein phosphatase type 1 (PP1) and type 2A (PP2A) that dephosphorylate serine and threonie residues in eukaryotic cells. Okadaic acid and calyculin A induce apoptosis in osteoblastic cells including Saos-2, MG63 and MCeTe-E1 cells. cDNA cloning revealed the existence of four isoforms of PP1 catalytic subunit in rat, termed PP1α, PP1γ1, PP1γ2, and PP1δ. PP1 targeting subunits is thought to localize to specific subcellular component and to modulate the activity of the enzyme at these sites. Nucleolin is an abundantly expressed nucleolar phosphoprotein and is located mainly in nucleolus. Staining pattern of nucleolin in MG63 cells is similar to that of the PP1δ. The dual fluorescence image revealed that PP1δ and nucleolin represent same localization in nucleolus. The anti-nucleolin antibody interacted with the 100 kDa protein immunoprecipitated with PP1δ antibody. However, anti-nucleolin antibody did not interact with the samples precipitated with the normal rabbit serum. The 100 kDa protein was dephosphorylate into 98 kDa proteins by lambda phosphatase. In the actinomycin D-treated cells, subcellular localization of PP1δ and nucleolin was changed. The amount of PP1δ increased whereas the level of dephosphorylated form of nucleolin increased. These results indicate that PP1δ associate with nucleolin directly to dephosphorylate this protein and is involved in r-RNA synthesis.
一些证据表明,蛋白质磷酸化和去磷酸化已被认为是各种组织中细胞增殖、分化和凋亡的关键机制。冈田酸是由甲藻产生的有毒聚醚脂肪酸,并且是蛋白磷酸酶1型(PP 1)和2A型(PP 2A)的有效抑制剂,其使真核细胞中的丝氨酸和苏氨酸残基去磷酸化。冈田酸和calyculin A诱导成骨细胞包括Saos-2、MG 63和MCeTe-E1细胞凋亡。cDNA克隆显示大鼠存在4种PP 1催化亚基的异构体,分别命名为PP 1 α、PP 1 γ1、PP 1 γ2和PP 1 δ。PP 1靶向亚基被认为定位于特定的亚细胞组分并调节这些位点的酶活性。核仁素是一种高表达的核仁磷蛋白,主要定位于核仁中。核仁素在MG 63细胞中的染色模式与PP 1 δ相似。双重荧光图像显示,PP 1 δ和核仁素在核仁中具有相同的定位。抗核仁素抗体与PP 1 δ抗体免疫沉淀的100 kDa蛋白相互作用。但抗核仁素抗体与正常兔血清沉淀的样品不发生相互作用。100 kDa的蛋白被λ磷酸酶脱磷酸化为98 kDa的蛋白。在放线菌素D处理的细胞中,PP 1 δ和核仁素的亚细胞定位发生变化。PP 1 δ的量增加,而核仁素的去磷酸化形式的水平增加。这些结果表明,PP 1 δ与核仁素直接结合,使该蛋白脱磷酸化,并参与r-RNA的合成。
项目成果
期刊论文数量(54)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Y.Muraki: "Malignant peripheral nerve sheath tumour in the maxilla associated with von Recklinghausen's disease : A case report."Oral Diseases. 5. 250-252 (1999)
Y.Muraki:“与冯·雷克林豪森病相关的上颌恶性周围神经鞘瘤:病例报告。”口腔疾病。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
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- 通讯作者:
C.Seta: "Fas expression and Fas monoclonal antibody-induced apoptosis in human squamous cell carcinoma cell line SCC-25 cells."Journal of Oral Pathology and Medicine. 29(6). 271-278 (2000)
C.Seta:“人鳞状细胞癌细胞系 SCC-25 细胞中的 Fas 表达和 Fas 单克隆抗体诱导的细胞凋亡。”口腔病理学与医学杂志。
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- 影响因子:0
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Y.Morimoto: "Quantitative radiographic changes in the mandible and the tibia in systematically loaded rats fed a low-calcium diet."Oral Diseases. 6(5). 310-317 (2000)
Y.Morimoto:“喂食低钙饮食的系统负荷大鼠的下颌骨和胫骨的定量放射照相变化。”口腔疾病。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
T.Haneji: "Protein phosphatases and apoptosis : Nuclear protein associates with protein phosphatase type 1δ isoform (in Japanese)"Shikoku Dental Research. 13(2). 221-229 (2001)
T.Haneji:“蛋白磷酸酶和细胞凋亡:核蛋白与 1δ 型蛋白磷酸酶亚型相关”四国牙科研究 13(2) (2001)。
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- 影响因子:0
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Y.Morimoto: "Alteration of argyrophilic nucleolar organizer region associated (Ag-NOR) proteins in apoptosis-induced human salivary gland cells and human squamous carcinoma cells."Journal of Oral Pathology and Medicine. (in press).
Y.Morimoto:“凋亡诱导的人唾液腺细胞和人鳞状癌细胞中嗜银核仁形成区相关 (Ag-NOR) 蛋白的改变。”口腔病理学与医学杂志。
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HANEJI Tatsuji其他文献
HANEJI Tatsuji的其他文献
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{{ truncateString('HANEJI Tatsuji', 18)}}的其他基金
The role of PKR on the differentiation of osteoblasts and formation of osteoclasts
PKR对成骨细胞分化和破骨细胞形成的作用
- 批准号:
25462859 - 财政年份:2013
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The role of PKR, Osterix, and Calcineurin in osteogenesis
PKR、Osterix 和钙调磷酸酶在成骨中的作用
- 批准号:
21592330 - 财政年份:2009
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Elucidation of the mechanisms of okadaic acid-induced apoptosis and cleavage of nucleolar proteins in osteoblasts
阐明冈田酸诱导成骨细胞凋亡和核仁蛋白裂解的机制
- 批准号:
17591915 - 财政年份:2005
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Protein phosphatases and cleavage of nucleolin in apoptosis-induced osteoblastic cells
凋亡诱导的成骨细胞中的蛋白磷酸酶和核仁素裂解
- 批准号:
15591936 - 财政年份:2003
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Protein Dephosphorylation and Apoptosis in Osteoblast
成骨细胞中的蛋白质去磷酸化和细胞凋亡
- 批准号:
09671860 - 财政年份:1997
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Development of monoclonal antibody for human sperm protein
人精子蛋白单克隆抗体的研制
- 批准号:
05671300 - 财政年份:1993
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
Evaluation of immunological infertility using monoclonal antibody
使用单克隆抗体评估免疫性不孕症
- 批准号:
02670733 - 财政年份:1990
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
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Fas antigen and Fas ligand with signaling pathway in Squamous carcinoma cells
鳞状癌细胞中Fas抗原和Fas配体与信号通路的关系
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Expression and function of Fas antigen in human oral epithelium.
Fas 抗原在人口腔上皮中的表达和功能。
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08672324 - 财政年份:1996
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