Study of the effects of bisphenol A in the dental pulp tissue

为了检测腹腔注射后小鼠牙髓组织中双酚A（BPA）的摄入量，我们对匀浆的牙髓组织进行了HPLC分析。8周龄雄性小鼠腹腔注射BPA（100 μg/g体重），分别于注射后半小时和1小时从下切牙中提取牙髓组织。注射后半小时，牙髓组织中BPA含量为100μg/g，注射后1h保持该水平。注射后半小时，血清BPA水平达1μg/μl，并持续1小时，提示BPA通过血液系统进入牙髓组织，并在牙髓组织中蓄积。

In order to examine the amount of bisphenol A (BPA) taken in the dental pulp tissues of mouse after peritoneal injection, we performed the HPLC analysis in the homogenized dental pulp tissues. The eight weeks old male mice were peritoneally injected BPA (100 μg/g body weight), and the dental pulp tissues were extracted from lower incisors after half and one hour of the injection, respectively. Then serums were also collected to analyze the amount of BPA.The BPA levels of the dental pulp tissues indicated 100μg/g after half hour of the injection, and the levels were kept for one hour after the injection. On the other hand, serum levers of BPA reached to 1μg/μl after half hour of the injection and the levels were kept for one hour after the injection.These results suggest that BPA is taken in the dental pulp tissue throughout the blood system and may be accumulated in the dental pulp tissue.