Attempts to identify the hard tissue regeneration by human dental pulp stem cells

一般来说，未分化间充质细胞在人类牙髓组织中大量存在，并且已知含有大量的干细胞。然而，牙髓干细胞的特性和分化信号仍不清楚。因此，我们确定了作为干细胞表面标志的CD44在人DPSCs上的表达。CD44的表达约为60%。然后，CD44的配体透明质酸诱导碱性磷酸酶的表达。这些数据提示CD44信号可能诱导DPSCs有效的钙化。

In general, undifferentiated mesenchymal cells are in abundant supply in human dental pulp tissue, and it is known that containing a large number of stem cells. However, the clarification of characteristics of dental pulp stem cells (DPSCs) and differentiation signaling are still unknown. Therefore, We determined expression of CD44 that is known as a stem cell surface marker upon human DPSCs. The CD44 expression was approximately 60%. Then, hyaluronic acid that is a ligand of CD44 treatment induced expression of alkaline phosphatase. These data suggested that CD44 signaling may induce efficient calcification on DPSCs.