The analysis and significance of the autoderived-growthfactor as a stimulator of the proliferation and the differentiation of human dental pulp stem cells

研究了洗涤血小板提取物(WPLT)对人牙髓干细胞增殖和钙化的刺激作用。富血小板血浆(PRP)对牙髓细胞生长的刺激作用较弱，而WPLT对牙髓细胞生长的刺激作用较强。此外，缺乏血小板的血浆(PPP)可剂量依赖性地抑制培养的人牙髓细胞的生长。WPLT对β-甘油磷酸和抗坏血酸诱导的牙髓细胞钙化有促进作用，而WPLT单独作用不明显。β-甘油磷酸和抗坏血酸对骨-唾液酸蛋白和牙本质唾液蛋白的表达均无明显的促进作用，β-甘油磷酸和抗坏血酸对骨-唾液酸和抗坏血酸的作用无明显的相加或抑制作用。这些结果表明，WPLT是一种强有力的人牙髓细胞刺激因子，并在骨钙化过程中发挥重要作用。

The efficacy of the extract of washed platelet (WPLT) as a stimulator of the proliferation and the calcification of human dental pulp stem cells was investigated. The growth of pulp cells was weakly stimulated by treatment with platelet-rich plasma (PRP), while it was more effectively stimulated by WPLT. Moreover, platelet-poor plasma (PPP) dose-dependently inhibited the growth of cultured human dental pulp cells. WPLT stimulated the calcification induced by β-glycerophosphate and ascorbic acid in the pulp cells, although WPLT alone was ineffective. WPLT significantly stimulated the mRNA expression of bone Gla protein (BGP), but not that of bone sialoprotein (BSP) or dentin sialoprotein (DSP), β-glycerophosphate and ascorbic acid stimulated both BGP and DSP, and addition of WPLT with BGP and Aa did not produce any addition or ayregic effect of the mRNA expression by β-glycerophosphate and ascorbic acid. These results suggest that WPLT is a potent stimulator of human dental pulp cells, and play a significant role in bone calcification.