Addition of osteoconductivity to self-setting GBR membrane

本研究的目的是评估海藻酸钠和氯化钠反应后立即形成的海藻酸钠膜作为GBR自固化屏障膜的有用性。我们发现，当骨缺损填充海藻酸钠水溶液，然后滴加氯化钠水溶液时，海藻酸钠膜在骨缺损表面形成而不清除。术后2、4、8周取骨缺损处的大鼠胫骨，用3%海藻酸钠水溶液和3%氯化钠水溶液处理的实验组骨再生良好，对照组骨缺损处未应用GBR，骨缺损处由结缔组织填充。结果表明，海藻酸钠膜是一种良好的骨再生屏障膜，但用10%海藻酸钠水溶液和3%氯化钠水溶液制备海藻酸钠膜时，发现新骨发生变形，说明海藻酸钠膜的制备条件对骨再生效果有重要影响。此外，我们发现，当预先制备藻酸盐膜并应用于骨缺损时，骨缺损皮肤部位的骨形成较差。

The aim of this study is to evaluate the usefulness of alginate membrane that can be formed immediately upon the reaction of sodium alginate and sodium chloride as an self setting barrier membrane of GBR.We found alginate membrane was formed on the surface of bone defect without making clearance when the bone defect was filled with sodium alginate aqueous solution followed by dropping sodium chloride aqueous solution. When the tibiae of rats where GBR was made was taken out from the experimental animals 2, 4 and 8 weeks after surgery, we found bone was regenerated in the experimental group using 3% sodium alginate aqueous solution and 3% sodium chloride aqueous solution.In the case of control group which bone defect was made and applied no GBR, bone defect was filled with connective tissue. The results cleary demonstrated that alginate membrane is useful as barrier membrane of GBR.However, we found new bone was deformed when alginate membrane was made with using 10% sodium alginate aqueous solution and 3% sodium chloride aqueous solution, indicating that condition to prepare alginate membrane is important for the better bone regeneration. Also, we found poor bone formation on the skin site of bone defect when alginate membrane was prepared in advance and applied to the bone defect.