Addition of osteoconductivity to self-setting GBR membrane

本研究的目的是评价海藻酸钠与氯化钠反应后立即形成的海藻酸盐膜作为GBR自凝屏障膜的有效性。我们发现用海藻酸钠水溶液填充骨缺损后再滴入氯化钠水溶液，在骨缺损表面形成海藻酸盐膜而不清除。术后2周、4周、8周取出制备GBR的大鼠胫骨，实验组用3%海藻酸钠水溶液和3%氯化钠水溶液均可再生骨。对照组制备骨缺损，不应用GBR，骨缺损以结缔组织填充。结果表明，海藻酸盐膜可以作为GBR的屏障膜。然而，我们发现用10%的海藻酸钠水溶液和3%的氯化钠水溶液制备海藻酸盐膜时，新骨变形，说明制备海藻酸盐膜的条件对更好的骨再生很重要。同时，我们发现预先制备海藻酸盐膜并应用于骨缺损时，骨缺损皮肤部位骨形成不良。

The aim of this study is to evaluate the usefulness of alginate membrane that can be formed immediately upon the reaction of sodium alginate and sodium chloride as an self setting barrier membrane of GBR.We found alginate membrane was formed on the surface of bone defect without making clearance when the bone defect was filled with sodium alginate aqueous solution followed by dropping sodium chloride aqueous solution. When the tibiae of rats where GBR was made was taken out from the experimental animals 2, 4 and 8 weeks after surgery, we found bone was regenerated in the experimental group using 3% sodium alginate aqueous solution and 3% sodium chloride aqueous solution.In the case of control group which bone defect was made and applied no GBR, bone defect was filled with connective tissue. The results cleary demonstrated that alginate membrane is useful as barrier membrane of GBR.However, we found new bone was deformed when alginate membrane was made with using 10% sodium alginate aqueous solution and 3% sodium chloride aqueous solution, indicating that condition to prepare alginate membrane is important for the better bone regeneration. Also, we found poor bone formation on the skin site of bone defect when alginate membrane was prepared in advance and applied to the bone defect.