Periosteal regeneration and bone augmentation using recombinant human BMP-2/atelocollagen onlay implant

使用重组人 BMP-2/去端肽胶原嵌体植入物进行骨膜再生和骨增强

• 批准号: 11672000
• 负责人: MURATA Masaru
• 金额: $ 1.86万
• 依托单位: Health Sciences University of Hokkaido
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11672000/
• 关键词: BMP; Collagen; Periosteum; Bone augmentation; Regeneration; Implant; 骨再生

The efficacy of limited exposure of bone marrow by cortical perforations were evaluated in subperiosteal rhBMP-2 implant system. Ten μg of rhBMP-2 solution and 10 mg of pepsindigested collagen (atelocollagen) solution were mixed in the sterilized tube and lyophilized. The composition of rhBMP-2 (10 μg)/collagen (10 mg) was implanted into the space between the periosteum and skull with or without outer cortical perforations in 10-month old Wistar rats. At 1, 2, 3 and 4 weeks after operation, the implant and the skull were removed en bloc and decalcified coronal sections (4 μm in thickness) were made and stained with hematoxylin and eosin and Elastica van Gieson. RhBMP-2/collagen implant with cortical perforations resulted in an initial acceleration of osteogenesis, whereas the rhBMP-2/collagen without perforations resulted in peripheral bone with many round empty spaces in the middle area at 1 and 2 weeks. In addition, the rhBMP-2/collagen implant was completely replaced by newly formed bone at 4 weeks. Our present results indicate that limited cortical petforations to host bed contributed to promote osteogenesis in initial stages and this novel rhBMP-2/atelocollagen implant system, an absorbable device replaced by new bone, would lead to improved and more predictable treatments for bone augmentation.

在骨膜下 rhBMP-2 植入系统中评估了皮质穿孔有限暴露骨髓的功效。将10μg rhBMP-2溶液和10mg胃蛋白酶消化的胶原（去端肽胶原）溶液在灭菌管中混合并冻干。将 rhBMP-2 (10 μg)/胶原蛋白 (10 mg) 的组合物植入 10 个月大的 Wistar 大鼠的骨膜和颅骨之间的空间，无论是否有外皮质穿孔。术后1、2、3和4周，将植入物和颅骨整体取出，制作脱钙冠状切片（厚度4μm）并用苏木精、伊红和Elastica van Gieson染色。具有皮质穿孔的RhBMP-2/胶原蛋白植入物导致成骨的初始加速，而没有穿孔的rhBMP-2/胶原蛋白在1周和2周时导致外围骨在中间区域出现许多圆形空腔。此外，rhBMP-2/胶原蛋白植入物在 4 周时完全被新形成的骨所取代。我们目前的结果表明，宿主床的有限皮质穿孔有助于促进初始阶段的成骨，而这种新型 rhBMP-2/去端胶原植入系统（一种被新骨取代的可吸收装置）将导致骨增量治疗的改进和更可预测。

项目成果

Masaru Murata: "Bone augmentation by recombinant human BMP-2 and collagen on adultrat parietalbone."Int.J.Oral Maxillofac.Surg.. 28. 232-237 (1999)

Masaru Murata：“通过重组人 BMP-2 和胶原蛋白对成年大鼠顶骨进行骨增强。”Int.J.Oral Maxillofac.Surg.. 28. 232-237 (1999)