Bioassay in the bone-inducing system using matrix including neovascularization related genes.

使用包括新血管形成相关基因的基质进行骨诱导系统中的生物测定。

• 批准号: 16592015
• 负责人: MURATA Masaru
• 金额: $ 1.86万
• 依托单位: Health Sciences University of Hokkaido
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16592015/
• 关键词: bone induction; neovascularization; BMP-2; collagen; VEGF; gene; bone regeneration; BMP-2; 遣伝子

Two microgram of recombinant human bone morphogenetic protein-2 and type I atellocollagen (10mg) was implanted in the subcutaneous tissues of 4-week, 10-month and 18-month-old rats. The implants were removed at 1, 2 and 3 weeks after implantation. The effects of rat age on ectopic bone formation were evaluated on the explants using haematoxylin-eosin staining, morphometric analysis, alkaline phosphatase activity and calcium content determination, as well as immunohistochemical staining of type IV collagen present in the basement membrane of blood vessels. At 2 and 3 weeks, bone was observed in 4-week and 10-month-old rats, but the amount of bone formed in the latter was less than in the 4-week-old rats. In 18-month-old rats, bone was first found focally in limited regions of the explants at 3 weeks and the amount of bone was much less than in 4-week-old rats. At 1 week, blood vessels in the explants of 4-week-old rats were observed more than in those, of 10-month or 18-month-old rats. At 2 and 3 weeks, blood vessels were found in the central regions of the explants in 4-week-old rats more than in the same regions in 10-month or 18-month-old rats. These findings indicate that the rate and quantity of ectopic bone formation are reduced in aged rats, and suggest that the difference in blood vessel distribution is related to this reduction in ectopic bone formation.

将2微克重组人骨形态发生蛋白2和I型去端胶原（10毫克）植入4周、10个月和18个月龄大鼠的皮下组织中。植入物在植入后 1、2 和 3 周被移除。使用苏木精-伊红染色、形态分析、碱性磷酸酶活性和钙含量测定，以及血管基底膜中存在的 IV 型胶原的免疫组织化学染色，评估大鼠年龄对外植体异位骨形成的影响。第2周和第3周时，4周龄和10月龄大鼠观察到骨形成，但后者的骨形成量少于4周龄大鼠。在 18 个月大的大鼠中，3 周时首次在外植体的有限区域中发现骨，并且骨量比 4 周大的大鼠少得多。第 1 周时，4 周龄大鼠的外植体中观察到的血管多于 10 个月或 18 个月大的大鼠。第 2 周和第 3 周时，4 周龄大鼠外植体中央区域的血管数量多于 10 个月或 18 个月龄大鼠的同一区域。这些发现表明，老年大鼠异位骨形成的速度和数量减少，并表明血管分布的差异与异位骨形成的减少有关。

项目成果

生体模倣傾斜機能アパタイトの開発 -rhBNP-2による骨誘導-

仿生功能分级磷灰石的开发-rhBNP-2-骨诱导-

• 发表时间: 2005
• 期刊: 日口腔インプラント誌 18
• 作者: 佐々木 智也;村田 勝 ら
• 通讯作者: 村田 勝 ら

Bio-absorption and osteoinduction innovation of bone morphogenetic proteinsupported functionally graded apatites originated from cattle bone.

骨形态发生蛋白支持的牛骨功能分级磷灰石的生物吸收和骨诱导创新。

• 发表时间: 2005
• 期刊: J.Am.Ceram.Soc. 88
• 作者: T.Akazawa;M.Murata;et al.
• 通讯作者: et al.

Biodegradation and bioabsorption innovation of the functionally graded cattle-bone-originated apatite with blood compatibility

具有血液相容性的功能分级牛骨磷灰石的生物降解和生物吸收创新

• 发表时间: 2006
• 期刊: J Biomed Mater Res 76A
• 作者: T.Akazawa;M.Murata;et al.
• 通讯作者: et al.

Blood permeability of a novel ceramic scaffold for bone morphogenetic protein-2

• DOI: 10.1002/jbm.b.30686
• 发表时间: 2007-05-01
• 期刊: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
• 影响因子: 3.4
• 作者: Murata, M.;Akazawa, T.;Arisue, M.
• 通讯作者: Arisue, M.

Bone engineering -Biological materials and bone morphogenetic proteins-

骨工程-生物材料和骨形态发生蛋白-

• 发表时间: 2004
• 期刊: Phosphorus research bulletin 17
• 作者: Suzuki K.;Yamazaki;Sezaki;Uchinuma E.;Masaru Murata
• 通讯作者: Masaru Murata