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Development of new method for the treatment of halitosis using molecular biological technique

利用分子生物学技术开发治疗口臭的新方法

基本信息

批准号：
11672051
负责人：
OHO Takahiko
金额：
$ 2.24万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11672051/
关键词：
Halitosis Volatile sulfur compounds Hydrogen sulfide Methyl mercaptan Tongue coating Periodontitis

项目摘要

Oral malodor is mainly associated with the condition of the oral cavity and is originates from a variety of products produced by the bacterial metabolism of amino acids in materials such as food debris, desquamated cells from oral mucosa and leukocytes that accumulate in the mouth. These metabolites include volatile sulfur compounds (VSC), indole, skatole, amines and ammonia. Of these compounds, VSC are the main cause of oral malodor.In this study, the characteristics of patients complaining of halitosis were examined and the diagnostic accuracy of methods of measuring halitosis was evaluated. The organoleptic test revealed that 55% of the subjects had no or slight odor. There was a significant correlation between the organoleptic score and the total VSC level determined by other methods. The amount of tongue coating was significantly greater in the halitosis-positive group than in the halitosis-negative group, whereas there was no difference in the salivary flow rate between the two groups.The ability of periodontopathic Porphyromonas gingivalis to produce methyl mercaptan was examined. The invasive strain W83 and W50 produced large amounts of methyl mercaptan. We cloned and sequenced the gene encoding L-methionine-α-deamino-γ-mercaptomethane-lyase (METase) from P.gingivalis W83. We also constructed a METase-deficient mutant of P.gingivalis W83. The mutant showed a marked decrease in the formation of methyl mercaptan from L-methionine and decreased virulence compared with the wild-type strain W83. Inactivation of METase may be effective for the inhibition of oral malodor.

口腔恶臭主要与口腔的状况有关，并且源自于由材料中的氨基酸的细菌代谢产生的各种产物，例如食物残渣、来自口腔粘膜的脱落细胞和积聚在口腔中的白细胞。这些代谢物包括挥发性硫化合物（VSC）、吲哚、粪臭素、胺和氨。在这些化合物中，VSC是口腔恶臭的主要原因。在这项研究中，患者抱怨口臭的特点进行了检查，并评估了测量口臭的方法的诊断准确性。感官测试显示，55%的受试者没有或轻微的气味。感官评分与其他方法测定的总VSC水平之间存在显著相关性。舌苔量在口臭阳性组显著大于口臭阴性组，而唾液流速在两组之间没有差异。牙周病牙龈卟啉单胞菌的能力，以产生甲基唾液酸进行了检查。入侵菌株W83和W50产生大量的甲基苯丙胺。克隆了牙龈卟啉单胞菌W83的L-甲硫氨酸-α-脱氨基-γ-巯基甲烷裂解酶（METase）基因并进行了序列测定。我们还构建了牙龈卟啉单胞菌W83的METase缺陷突变体。与野生型菌株W83相比，突变体显示出从L-甲硫氨酸形成甲基硫氨酸的显著减少和毒力降低。METase的失活可能对抑制口腔恶臭有效。