Research of cell physiology and cell death in human culture cells under high-pressure.

研究高压下人体培养细胞的细胞生理学和细胞死亡。

• 批准号: 11672253
• 负责人: TAKANO Takako
• 金额: $ 2.24万
• 依托单位: Teikyo University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11672253/
• 关键词: high-pressure; cell death; apoptosis; lymphoblats; HL-60; caspase-3; tubulin; heat shock protein; 微小管; caspase

The human lymphoblasts (EB3) and human promyelocytic leukemia cells (HL-60) pressurized for 30 minutes at 37℃ were investigated. The pressure at which 50% cell viability of HL-60 is attained was lower than that of EB3. While, in EB3 cells, apoptotic cells began to be observed from 8 hours after decompression at 100 MPa, in HL-60 cells, they were observed immediately after decompression. In EB3 cells, a high-pressure signal overexpressed p53, which induced caspase-3 processing. In HL-60 cells, phospholylation of p38 MAPK was induced by high-pressure, participating in the common caspase cascade. The pressure-induced attenuation in α-tubulin and β-tubulin expression was observed after pressure treatment of 100 MPa for 30 minutes both by Western blot analysis and by 2-dimentional gel electrophoresis. We proposed that microtubules play a role in signal transduction executing apoptosis. The expression of several proteins increased immediately after decompression by 2-dimentional gel electrophoresis. One of them was revealed HSP60 (heat shock protein 60). HSP60 may mediate folding reaction under the high-pressure stress.

研究了人淋巴母细胞（EB3）和人早幼粒细胞白血病细胞（HL-60）在37℃下加压30分钟的反应。HL-60达到50%细胞活力时的压力低于EB3。而在EB3细胞中，在100 MPa下减压后8小时开始观察到凋亡细胞，在HL-60细胞中，在减压后立即观察到凋亡细胞。在EB3细胞中，高压信号过度表达p53，从而诱导caspase-3加工。在HL-60细胞中，高压诱导p38 MAPK磷酸化，参与常见的caspase级联反应。Western blot和二维凝胶电泳观察100 MPa压力作用30 min后α-微管蛋白和β-微管蛋白表达的衰减。我们提出微管在执行细胞凋亡的信号转导中发挥作用。二维凝胶电泳减压后，几种蛋白的表达量立即增加。其中1例检测到HSP60（热休克蛋白60）。HSP60可能介导高压应力下的折叠反应。

项目成果

T.Takano,K.J.Takano,Y.Yamanouchi.(分担): "SCIENCE AND TECHNOLOGY OF HIGH PRESSURE Proceedings of AIRAPT-17.Volume 1"Universities Press (India) Ltd.. 1-584 (2000)

T.Takano、K.J.Takano、Y.Yamanouchi.（共享）：《高压科学技术论文集 AIRAPT-17.Volume 1》Universities Press (India) Ltd.. 1-584 (2000)

T.Takano,K.J.Takano,Y.Yamanouchi.(分担): "SCIENCE AND TECHNOLOGY OF HIGH PRESSURE Proceedings of AIRAPT-17. Volume 1"Universities Press (India) Ltd.. 1-584 (2000)

T.Takano、K.J.Takano、Y.Yamanouchi.（共享）：“AIRAPT-17 高压科学与技术论文集。第 1 卷”Universities Press (India) Ltd.. 1-584 (2000)

T.Takano, K.J.Takano, Y.Yamanouchi: "Mechanism of pressure-induced apoptosis in human cells."SCIENCE AND TECHNOLOGY OF HIGH PRESSURE Proceedings of AIRAPT-17.. Volume 1. 342-345 (2000)

T.Takano、K.J.Takano、Y.Yamanouchi：“压力诱导人类细胞凋亡的机制。”AIRAPT-17 高压论文集的科学与技术.. 第 1 卷. 342-345 (2000)

T.Takano, K.J.Takano, Y.Yamanouchi: "Pressure-induced apoptosis in human culture cells."High-pressure bioscience and biotechnology.. 105-117

T.Takano、K.J.Takano、Y.Yamanouchi：“人类培养细胞中压力诱导的细胞凋亡。”高压生物科学和生物技术.. 105-117

Kaoru J.Takano: "Pressure-induced apoptosis in human lymphoblasts"Exp.Cell.Res.. 235. 155-160 (1997)

Kaoru J.Takano：“人淋巴母细胞中压力诱导的细胞凋亡”Exp.Cell.Res.. 235. 155-160 (1997)